Method to distinguish between the MMAC1/PTEN gene and its pseudogene in RT-PCR analysis of point mutations

BioTechniques

Volumn 26, Issue 1, 1999, Pages 19-23

  Liu, Juehui ^a   Kagan, Jacob ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; RESTRICTION ENDONUCLEASE;

ARTICLE; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; POINT MUTATION; PSEUDOGENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE; TUMOR SUPPRESSOR GENE;

EID: 0032895539     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99261bm02     Document Type: Article

References (16)

1. Cairns, P., K. Okami, S. Halachmi, N. Halachmi, M. Esteller, J.G. Herman, J. Jen, W.B. Isaacs, S.G. Bova and D. Sidransky. 1997. Frequent inactivation of PTEN/MMAC1 in primary prostate cancer. Cancer Res. 57:4997-5000.
2. Dahia, P.L.M., D.J. Marsh, Z. Zheng, J. Zedenius, P. Komminoth, T. Frisk, G. Wallin, R. Parsons et al. 1997. Somatic deletions and mutations in the Cowden disease gene, PTEN, in sporadic thyroid tumors. Cancer Res. 57:4710-4713.
3. Guldberg, P., P.T. Straten, A. Brick, V. Ahrenkiel, A.F. Kirkin and J. Zeuthen. 1997. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57:3660-3663.
4. Kim, S.K., L.K. Su, Y. Oh, B.L. Kemp, W.K. Hong and L. Mao. 1998. Alterations in PTEN1/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small lung cancer cell lines. Oncogene 16:89-93.
5. Kong, D., A. Suzuki, T.T. Zou, A. Sakurada, L.W. Kemp, S. Wakatsuki, H. Yokoyama, H. Yamakawa et al. 1997. PNTE1 is frequently mutated in primary endometial carcinomas. Nature Genet. 17:143-144.
6. Li, J., C. Yen, D. Liaw, K. Podsypanina, S. Bose, S.I. Wang, J. Puc, C. Miliaresis et al. 1997. PTEN, a putative protein tyrosin phosphatase gene mutated in human brain, breast, and prostrate cancer. Science 275:1943-1947.
7. Liaw, D., D.J. Marsh, J. Li, P.L.M. Dahia, S.I. Wang, Z. Zheng, S. Bose, K.M. Call et al. 1997. Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome. Nature Genet. 16:64-67.
8. Marsh, D.J., P.L.M. Dahia, Z. Zheng, D. Liaw, R. Parsons, R.J. Gorlin and C. Eng. 1997. Germline mutations in PTEN are present in Bannayan-Zonana syndrome. Nature Genet. 16:333-334.
9. Nelen, M.R., W.C.G. van Staveren, E.A.J. Peeters, M. Ben-Hassel, R.J. Gorlin, H. Hamm, C.F. Lindboe, J.P. Fryns et al. 1997. Germline mutations in the PTEN/MMAC1 gene in patients with Cowden disease. Hum. Mol. Genet. 6:1383-1387.
10. Olschwang, S., O.M. Serova-Sinilnikova, G.M. Lenior and T. Gilles. 1998. Germ-line mutations in juvenile polyposis coli. Nature Genet. 18:12-14.
11. Raff, T., M. van der Giet, D. Endemann, T. Wiederholt and M. Paul. 1997. Design and testing of β-actin primers for RT-PCR that do not co-amplify processed pseudogenes. BioTechniques. 23:456-460.
12. Risinger, J.I., K.A. Hayes, A. Berchuck and C.J. Barrett. 1997. PTEN/MMAC1 mutations in endometrial cancers. Cancer Res. 57:4736-4738.
13. Rhei, E., L. Kang, F. Bogomolniy, M.G. Federici, P.I. Borgen and J. Boyd. 1997. Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in primary breast cancer. Cancer Res. 57:3657-3659.
14. Steck, P.A., M.A. Pershouse, S.A. Jasser, A.W.K. Yung, H. Lin, A.H. Ligon, L.A. Langford, M.L. Baumgard et al. 1997. Identification of a candidate tumor suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nature Genet. 15:356-362.
15. Suzuki, H., D. Freije, D.R. Nusskern, K. Okami, P. Cairns, D. Sidransky, W.B. Isaacs and S.G. Bova. 1997. Interfocal heterogenicity of MMAC1/PTEN gene alterations in multiple metastatic prostate cancer tissues. Cancer Res. 58:204-209.
16. Teng, D.H.-F., R. Hu, H. Lin, T. Davis, D. Iliev, C. Frye, B. Swedlund, K.L. Hansen et al. 1997. MMAC1/PTEN mutations in primary tumor specimens and tumor cell lines. Cancer Res. 57:5221-5225.