RecA-mediated affinity capture: A method for full-length cDNA cloning

BioTechniques

Volumn 27, Issue 4, 1999, Pages 834-845

  Zhumabayeva, Bakhyt ^a   Chenchik, Alex ^a   Siebert, Paul D ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; PROTEIN BCL X; RECA PROTEIN;

ARTICLE; BIOTECHNOLOGY; BIOTINYLATION; DNA LIBRARY; ESCHERICHIA COLI; HUMAN; MOLECULAR CLONING; PLASMID; PRECIPITATION; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI; PROKARYOTA;

EID: 0032882447     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99274rr06     Document Type: Article

References (18)

1. Bianco, P.R. and G.M. Weinstock. 1996. Interaction of the RecA protein of Escherichia coli with single-stranded oligodeoxyribonucleotides. Nucleic Acids Res. 24:4933-4939.
2. Boise, L.H., M. Gonzales-Garcia, C.E. Postema, L. Ding, T. Lindsten, L.A. Turka, X. Mao, G. Nunez and C.B. Thompsom. 1993. Bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 74:597-608.
3. Chenchik, A., L. Diachenko, F. Moqadam, V. Tarabykin, S. Lukyanov and P.D. Siebert 1996. Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques 21:526-534.
4. Dole, M.G., M.F. Clarke, P. Holman, M. Benedict, J. Lu, R. Jasty, P. Eipers, C.B. Thompson et al. 1996. Bcl-xS enhances adenoviral vector-induced apoptosis in neuroblastoma cells. Cancer Res. 56:5734-5740.
5. Hagen, F.S., C.L. Gray and J.L. Kuijper. 1988. Assaying the quality of cDNA libraries. BioTechniques 6:340-345.
6. Hakvoort, T.B.M., J.A.A. Spijkers, J.L.M. Vermeulen and W.H. Lamers. 1996. Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments. Nucleic Acids Res. 24:3478-3480.
7. Hakvoort, T.B.M., J.L.M. Vermeulen and W.H. Lamers. 1998. Enriched full-length cDNA expression library by RecA-mediated affinity capture, p. 259-269. In P.D. Siebert and J.W. Larrick (Eds.), Gene Cloning and Analysis by RT-PCR. Eaton Publishing, Natick, MA.
8. Honigberg, S.M., B.J. Rao and C.M. Radding. 1986. Ability of RecA protein to promote a search for rare sequences in duplex DNA. Proc. Natl. Acad. Sci. USA 83:9586-9590.
9. Hsieh, P., C.S. Camerini-Otero and R.D. Camerini-Otero. 1992. The synapsis event in the homologous pairing of DNAs: RecA recognizes and pairs less than one helical repeat of DNA. Proc. Natl. Acad. Sci. USA 89:6492-6496.
10. Kowalczykowski, S.C. and A.K. Eggleston. 1994. Homologous pairing and DNA strand-exchange proteins. Annu. Rev. Biochem. 63:991-1043.
11. Rao, B.J., M. Dutreix and C.M. Radding. 1991. Stable three-stranded DNA made by RecA protein. Proc. Natl. Acad. Sci. USA 88:2984-2988.
12. Rigas, B., A.A. Welcher, D.C. Ward and S.M. Weissman. 1986. Rapid plasmid library screening using RecA-coated biotinylated probes. Proc. Natl. Acad. Sci. USA 83:9591-9595.
13. Schott, A.F., I.J. Apel, G. Nunez and M.F. Clarke. 1995. Bcl-xL protects cancer cells from p53-mediated apoptosis. Oncogene 11:1389-1394.
14. Sena, E.P. and D.A. Zarling. 1993. Targeting in linear DNA duplexes with two complementary probe strands for hybrid stability. Nat. Genet. 3:365-372.
15. Taidi-Laskowski, B., D. Tyan, S.M. Honigberg, C.R. Radding and F.C. Grumet. 1988. Use of RecA protein to enrich for homologous genes in genomic library. Nucleic Acids Res. 16:8157-8169.
16. Teintze, M., I.I. Arzimanoglou, C.I.P. Lovelace, Z. Xu and B. Rigas. 1995. RecA-assisted rapid enrichment of specific clones from model DNA libraries. Biochem. Biophys. Res. Comm. 211:804-811.
17. Tracy, R.B. and S.C. Kowalczykowski. 1996. In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein. Genes Dev. 10:1890-1903.
18. Zhang, Y. and M.A. Frohman. 1997. Using rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs, p. 61-87. In I.G. Cowel and C.A. Austin (Eds.), cDNA Library Protocols. Humana Press, Totowa, NJ.