Surface roughness modulates the response of MG63 osteoblast-like cells to 1,25-(OH)2D3 through regulation of phospholipase A2 activity and activation of protein kinase A

Journal of Biomedical Materials Research

Volumn 47, Issue 2, 1999, Pages 139-151

  Lohmann, C H ^a   Sagun Jr , R ^a,b   Sylvia, V L ^a   Cochran, D L ^a   Dean, D D ^a   Boyan, B D ^a,c   Schwartz, Z ^a,d  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c WILFORD HALL MEDICAL CENTER   (United States)

^d HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

1,25(OH)2D3; In vitro; Osteoblasts; Phospholipase A2; Protein kinase A; Protein kinase C; Surface roughness; Titanium

Indexed keywords

CELL CULTURE; CHEMICAL ACTIVATION; ENZYME INHIBITION; HORMONES; PROTEINS; SURFACE ROUGHNESS; TITANIUM;

OSTEOBLASTS; PHOSPHOLIPASE; PROTEIN KINASE C;

IMPLANTS (SURGICAL);

ALKALINE PHOSPHATASE; CALCITRIOL; CHELERYTHRINE; CYCLIC AMP DEPENDENT PROTEIN KINASE; MELITTIN; MEPACRINE; N (2 METHYLAMINOETHYL) 5 ISOQUINOLINESULFONAMIDE; PHOSPHOLIPASE A2; PROSTAGLANDIN E2; PROTEIN KINASE C; TITANIUM;

ARTICLE; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME REGULATION; HUMAN; HUMAN CELL; IMPLANT; OSTEOBLAST; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

BIOPROSTHESIS; BONE REGENERATION; CALCITRIOL; CALCIUM CHANNEL AGONISTS; CELL DIFFERENTIATION; CELL DIVISION; CELL LINE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HUMANS; OSTEOBLASTS; PHOSPHOLIPASES A; SIGNAL TRANSDUCTION;

EID: 0032875630     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4636(199911)47:2<139::AID-JBM4>3.0.CO;2-2     Document Type: Article

References (50)

1. Schwartz Z, Boyan BD. Underlying mechanisms at the bone-biomaterial interface. J Cell Biochem 1994;56:340-347.
2. Thomas KA, Cook SD. An evaluation of variables influencing implant fixation by direct bone apposition. J Biomed Mater Res 1985;19:875-901.
3. Buser D, Schenk R, Steinemann S, Fiorellini J, Fox C, Stich H. Influence of surface characteristics on bone integration of titanium implants: A histomorphometric study in miniature pigs. J Biomed Mater Res 1991;25:889-902.
4. Carlsson L, Rostlund T, Albrektsson B, Albrektsson T. Removal torques for polished and rough titanium implants. Int J Oral Maxillofac Implants 1988;3:21-24.
5. Wilke HJ, Claes L, Steinemann S. The influence of various titanium surfaces on the interface shear strength between implants and bone. In: Heimke G, Soltész U, Lee AJC, editors. Clinical implant materials: Advances in biomaterials. Amsterdam: Elsevier; 1990. p 309-314.
6. Michaels CM, Keller JC, Stanford CM, Solursh M. In vitro cell attachment of osteoblast-like cells to titanium. J Dent Res 1989; 68:276.
7. Bowers KT, Keller JC, Randolph BA, Wick DG, Michaels CM. Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Maxillofac Implants 1992;7: 302-310.
8. Martin JY, Schwartz Z, Hummert TW, Schraub DM, Simpson J, Lankford J, Dean DD, Cochran DL, Boyan BD. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 1995;29:389-401.
9. Lincks J, Boyan BD, Blanchard CR, Lohmann CH, Liu Y, Cochran DL, Dean DD, Schwartz Z. Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. Biomaterials 1998;19: 2219-2232.
10. Kieswetter K, Schwartz Z, Hummert TW, Cochran DL, Simpson J, Dean DD, Boyan BD. Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG63 cells. J Biomed Mater Res 1996;32:55-63.
11. 3. J Biomed Mater Res 1998;39:77-85.
12. 3 specific regulation of growth, morphology, and fibronectin in a human osteosarcoma cell line. J Cell Physiol 1985;123:401-409.
13. 3 responsive alkaline phosphatase in osteoblast-like cells (ROS 17/2.8, MG 63, and MC 3T3) and growth cartilage cells in culture. J Biol Chem 1989;264:11879-11886.
14. 3 on differentiation of a human osteosarcoma (MG-63). J Biol Chem 1992;267:8943-8949.
15. 3 in three osteoblast-like cell lines (MC-3T3-E1, ROS 17/2.8, and MG-63). Bone 1992;13:51-58.
16. 3. J Biomed Mater Res 1998;41:489-496.
17. 2 activity of growth zone and resting zone cartilage cells in vitro. Endocrinology 1988;122:2191-2198.
18. 3 in growth zone and resting zone chondrocyte cultures. Biochim Biophys Acta 1990;1027:278-286.
19. 2 production by vitamin D metabolites in growth zone and resting zone chondrocyte cultures is dependent on cell maturation. Bone 1992;13:395-401.
20. 3 on calcium ion fluxes in costochondral chondrocyte cultures. Calcif Tissue Int 1990;47:230-236.
21. 3 on growth plate chondrocytes. J Cell Physiol 1998;176:516-524.
22. Schwartz Z, Sylvia VL, Dean DD, Boyan BD. The synergistic effects of vitamin D metabolites and TGFβ on costochondral chondrocytes are mediated by increases in protein kinase C activity involving two separate pathways. Endocrinology 1998; 139:534-545.
23. 2 in osteoblast-like cells. J Cell Biochem 1993;52:220-226.
24. Civitelli R, Kim YS, Gunsten SL, Fujimori A, Huskey M, Avioli LV, Hruska KA. Nongenomic activation of the calcium message system by vitamin D metabolites in osteoblast-like cells. Endocrinology 1990;127:2253-2262.
25. 3 regulation of calcium uptake by cultured myocytes. Cell Signal 1994;6:531-538.
26. 3. J Cell Biochem 1994;56:510-517.
27. 3 which mediates rapid activation of protein kinase C. J Bone Miner Res 1998;13:1353-1359.
28. Gronowicz G, McCarthy MB. Response of human osteoblasts to implant materials: Integrin-mediated adhesion. J Orthop Res 1996;14:878-887.
29. Schlaepfer DD, Hunter T. Integrin signalling and tyrosine phosphorylation: Just the FAKs? Trends Cell Bioil 1998;8:151-157.
30. Bell RL, Kennerly DA, Stanford N, Marjerus PW. Diglyceride lipase: A pathway for arachidonate release from platelets. Proc Natl Acad Sci USA 1979;76:3238-3241.
31. Schwartz Z, Martin JY, Dean DD, Simpson J, Cochran DL, Boyan BD. Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation. J Biomed Mater Res 1996;30:145-155.
32. Herbert JM, Augereau JM, Gleye J, Maffrand JP. Chelerythrine is a potent and specific inhibitor of protein kinase C. Biochem Biophys Res Commun 1990;172:993-999.
33. iol 1996;169:509-521.
34. Habermann E. Bee and wasp venoms. Science 1972;177:314-322.
35. Schwartz Z, Gilley RM, Sylvia VL, Dean DD, Boyan BD. The effect of prostaglandin E2 on costochondral chondrocyte differentiation is mediated by cAMP and protein kinase C. Endocrinology 1998;139:1825-1834.
36. 2 activation, platelet-activating factor generation and prostacyclin release in spontaneously beating rat cardiomyocytes. Biochem J 1993;290:477-482.
37. 2 and phospholipase C in growth zone chondrocytes. J Bone Miner Res 1998;13:559-569.
38. Hale LV, Kemick ML, Wuthier RE. Effect of vitamin D metabolites on the expression of alkaline phosphatase activity by epiphyseal hypertrophic chondrocytes in primary cell culture. J Bone Miner Res 1986;1:489-495.
39. Bretaudiere JP, Spillman T. Alkaline phosphatases. In: Bergmeyer HU, editor. Methods of enzymatic analysis. Weinheim: Verlag Chemica; 1984. p 75-92.
40. Sylvia VL, Schwartz Z, Schuman L, Morgan RT, Mackey S, Gomez R, Boyan BD. Maturation-dependent regulation of protein kinase C activity by vitamin D3 metabolites in chondrocyte cultures. J Cell Physiol 1993;157:271-278.
41. Bell RM, Hannun Y, Loomis C. Mixed micelle assay of protein kinase C. Methods Enzymol 1986;124:353-359.
42. Negishi M, Sugimoto Y, Ichikawa A. Molecular mechanisms of diverse actions of prostanoid receptors. Biochim Biophys Acta 1995;1259:109-120.
43. 2 specific activities. Endocrinology 1988;123:2878-2884.
44. Kieswetter K, Schwartz Z, Dean DD, Boyan BD. The role of implant surface characteristics in the healing of bone. Crit Rev Oral Biol Med 1996;7:329-345.
45. Schwartz Z, Schlader DL, Ramirez V, Kennedy MB, Boyan BD. Effects of vitamin D metabolites on collagen production and cell proliferation of growth zone and resting zone cartilage cells in vitro. J Bone Miner Res 1989;4:199-207.
46. Stein GS, Lian JB, Owen TA. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J 1990;4:3111-3123.
47. Martin-Thouvenin V, Gendron MC, Hogervorst F, Figdor CG, Lanotte M. Phorbol ester-induced promyelocytic leukemia cell adhesion to marrow stromal cells involves fibronectin specific alpha 5 beta 1 integrin receptors. J Cell Physiol 1992;153:95-102.
48. Hollinger J, Marx DE, Bach DE, Reddi AH, Seyfer AE. Calvarial bone regeneration using osteogenin. J Oral Maxillofac Surg 1989;47:1182-1187.
49. Madden K, Sheu YJ, Baetz K, Andrews B, Snyder M. SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway. Science 1997;275:1781-1784.
50. Kanner SB, Grosmaire LS, Ledbetter JA, Damle NK. Beta 2-integrin LFA-1 signaling through phospholipase C-gamma 1. Proc Natl Acad Sci USA 1993;90:7099-7103.