Assessment of the essentiality of ERG genes late in ergosterol biosynthesis in Saccharomyces cerevisiae

Current Genetics

Volumn 32, Issue 2, 1997, Pages 93-99

  Palermo, Lizette M ^a   Leak, Frank W ^a   Tove, Shirley ^a   Parks, Leo W ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

ERG genes; Ergosterol; Saccharomyces; Yeast

Indexed keywords

ERGOSTEROL; STEROL;

ARTICLE; FUNGAL GENETICS; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; STEROL SYNTHESIS;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; ERGOSTEROL; GENES, FUNGAL; METHYLTRANSFERASES; MUTATION; OXIDOREDUCTASES; SACCHAROMYCES CEREVISIAE; SELECTION (GENETICS);

EID: 0030770249     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050252     Document Type: Article

References (20)

1. Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M (1991) Cloning, disruption, and sequence of the gene encoding yeast C-5 sterol desaturase. Gene 102:39-44
2. Ashman WH, Barbuch RJ, Ulbright CE, Jarrett HW, Bard M (1991) Cloning and disruption of the yeast C-8 sterol isomerase gene. Lipids 26:628-632
3. Bloch K (1988) Sterol structure and function. J Am Oil Chemists Soc 65:1763-1766
4. Crowley JH, Smith SJ, Leak FW, Parks LW (1996) Aerobic isolation of an ERG24 null mutant of Saccaromyces cerevisiae. J Bacteriol 178:2291-2993
5. Fenner GP, Parks LW (1989) Gas Chromatographic analysis of intact steryl esters in wild-type Saccharomyces cerevisiae and in an ester-accumulating mutant. Lipids 24:625-629
6. Gaber RF, Copple DM, Kennedy BK, Vidal M, Bard M (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol 9:3447-3456
7. Hill JE, Meyers AM, Koerner TJ, Tzagoloff A (1986) Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167
8. Lai MH, Bard M, Pierson CA, Alexander JF, Goebl M, Carter GT, Kirsch DR (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140:41-49
9. Lorenz RT, Parks LW (1992) Cloning, sequencing and disruption of the gene encoding sterol C-14 reductase in Sacccharomyces cerevisiae. DNA Cell Biol 11:685-692
10. Manivasakam P, Weber SC, McElver J, Schiestl RH (1995) Microhomology mediated PCR targeting in Saccharomyces cerevisiae. Nucleic Acids Res 23:2799-2800
11. McCammon MT, Hartmann M-A, Bottema CDK, Parks LW (1984) Sterol methylation in Saccharomyces cerevisiae. J Bacteriol 157:475-483
12. Parks LW (1978) Metabolism of sterols in yeast. CRC Crit Rev Microbiol 6:301-341
13. Parks LW, Casey WM (1995) Physiological implications of sterol biosynthesis in yeast. Annu Rev Microbiol 49:95-116
14. Parks LW, Bottema CDK, Rodriguez RJ, Lewis TA (1985) Yeast sterols: yeast mutants as tools for the study of sterol metabolism. Methods Enzymol 111:333-346
15. Rodriguez, RJ, Low C, Bottema CDK, Parks LW (1985) Multiple functions for sterols in Saccharomyces cerevisiae. Biochim Biophys Acta 837:336-343
16. Rose MD, Waston F, Hieter P (1990) Methods in yeast genetics: a laboratory course manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
17. Rothstein R (1991) Targeting, disruption, replacement and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 194:281-301
18. Skaggs BA, Alexander JF, Peirson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M (1996) Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol. Gene 169:105-109
19. Smith SJ, Parks LW (1993) The ERG3 gene in Saccharomyces cere- visiae is required for utilization of respiratory substrates and in heme-deficient cells. Yeast 9:1177-1187
20. Smith SJ, Crowley JH, Parks LW (1996) Transcriptional regulation by ergosterol in the yeast, Saccharomyces cerevisiae. Mol Cell Biol 16:5427-5432