Growth properties of a folA null mutant of Escherichia coli K12

Canadian Journal of Microbiology

Volumn 45, Issue 3, 1999, Pages 191-200

  Herrington, Muriel B ^a   Chirwa, Neema T ^a  

^a Dept of Electrical and Computer Engineering   (Canada)

Author keywords

Biosynthesis; Dihydrofolate reductase; Escherichia coli; Folates; One carbon metabolism

Indexed keywords

4 AMINOBENZOIC ACID; DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; FOLIC ACID; KANAMYCIN; TETRAHYDROFOLIC ACID; THYMIDINE PHOSPHATE;

ARTICLE; BACTERIAL GROWTH; BIOSYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; GENE SEQUENCE; METABOLISM; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CULTURE MEDIA; DRUG RESISTANCE; ESCHERICHIA COLI; GENE DELETION; KANAMYCIN; MUTATION; PLASMIDS; TEMPERATURE; TETRAHYDROFOLATE DEHYDROGENASE; THYMIDINE; TIME FACTORS; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0032856008     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjm-45-3-191     Document Type: Article

References (59)

1. Ahrweiler, P., and Frieden, C. 1988. Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments. J. Bacteriol. 170: 3301-3304.
2. Alifano, P., Rivellini, F., Nappo, A.G., Bruni, C.B., and Carlomagno, M.S. 1994. Alternative patterns of his operon transcription and mRNA processing generated by metabolic perturbation. Gene, 146: 15-21.
3. Basso, J. 1993. The effect of perturbing folate metabolism on translational accuracy and growth of Escherichia coli. Ph.D. thesis, Concordia University, Montreal, Quebec.
4. Baumstark, B.R., Spremulli, L.L., RajBhandary, U.L., and Brown, G.M. 1977. Initiation of protein synthesis without formylation in a mutant of Escherichia coli that grows in the absence of tetrahydrofolate. J. Bacteriol. 129: 457-471.
5. Bertino, J.B., and Stacey, K.A. 1966. A suggested mechanism for selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem. J. 101: 32C-33C.
6. Blanchin-Roland, S., Blanquet, S., Schmitter, J.M., and Fayat, G. 1986. The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA. Mol. Gen. Genet. 205: 515-522.
7. Blattner, F.R., Plunkett, G., III, Bloch, C.A., Perna, N.T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J.D., Rode, C.K., Mayhew, G.F., Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J., Mau, B., and Shao, Y. 1997. The complete genome sequence of Escherichia coli K-12. Science (Washington, D.C.), 277: 1453-1474.
8. Bochner, B.R., and Ames, B.N. 1982. ZTP (5-amino 4-imidazole carboxamide riboside 5′-triphosphate): a proposed alarmone for 10-formyl-tetrahydrofolate deficiency. Cell, 29: 929-937.
9. Bognar, A.L., Osborne, C., and Shane, B. 1987. Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene. J. Biol. Chem. 262: 2337-2343.
10. Brown, R.E., Jarvis, K.L., and Hyland, K.J. 1989. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal. Biochem. 180: 136-139.
11. Chang, A.C., and Cohen, S.N. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156.
12. Cheung, P.K., and Herrington, M.B. 1982. Thymine inhibits suppression by an Escherichia coli nonsense and frameshift suppressor. Mol. Gen. Genet. 186: 217-220.
13. Csonka, L.N., and Clark, A.J. 1979. Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome. Genetics, 93: 321-343.
14. Fermér, C., and Swedberg, G. 1997. Adaptation to sulfonamide resistance in Neisseria meningitidis may have required compensatory changes to retain enzyme function: kinetic analysis of dihydropteroate synthases from N. meningitidis expressed in a knockout mutant of Escherichia coli. J. Bacteriol. 179: 831-837.
15. Gesteland, R.F. 1966. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J. Mol. Biol. 16: 67-84.
16. Gold, L. 1988. Posttranscriptional regulatory mechanisms in Escherichia coli. Annu. Rev. Biochem. 57: 199-233.
17. Green, J.M., Nichols, B.P., and Matthews, R.G. 1996. Folate biosynthesis, reduction, and polyglutamylation. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 665-673.
18. Greene, R.C. 1996. Biosynthesis of methionine. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 542-560.
19. Greener, A., and Hill, C.W. 1980. Identification of a novel genetic element in Escherichia coli K-12. J. Bacteriol. 144: 312-321.
20. Haertle, T., Wohlrab, F., and Guschlbauer, W. 1979. Thymidylate synthetase from Escherichia coli K12. Purification and dependence of kinetic properties on sugar conformation and size of the 2′ substituent. Eur. J. Biochem. 102: 223-230.
21. Hamm-Alvarez, S.F., Sancar, A., and Rajagopalan, K.V. 1990. The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J. Biol. Chem. 265: 9850-9856.
22. Herrington, M.B. 1994. Measurement of the uptake of radioactive para-aminobenzoic acid monitors folate biosynthesis in Escherichia coli K-12. Anal. Biochem. 216: 427-430.
23. Herrington, M.B., and MacRae, T.J. 1998. (Concordia University.) Unpublished data.
24. Herrington, M.B., Kohli, A., and Lapchak, P.H. 1984. Suppression by thymidine-requiring mutants of Escherichia coli K12. J. Bacteriol. 157: 126-129.
25. Herrington, M.B., Kohli, A., and Faraci, M. 1986. Frameshift suppression by thyA mutants of Escherichia coli K12. Genetics, 114: 705-716.
26. Herrington, M.B., Basso, J., Faraci, M., and Autexier, C. 1991. Modification of the suppressor phenotype of thymine requiring strains of Escherichia coli. Genet. Res. 58: 185-192.
27. Herrington, M.B., Wong, S.H., and Basso, J. 1998. (Concordia University.) Unpublished data.
28. Howell, E.E., Foster, P.G., and Foster, L.M. 1988. Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement. J. Bacteriol. 170: 3040-3045.
29. Jensen, K.F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyre expression levels. J. Bacteriol. 175: 3401-3407.
30. Kingsbury, D.T., and Helinski, D.R. 1970. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem. Biophys. Res. Commun. 41: 1538-1544.
31. Kohara, Y., Kiyotaka, A., and Isono, K. 1987. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genome library. Cell, 50: 495-508.
32. Krishnan, B.R., and Berg, D.E. 1993. Viability of folA-Null derivatives of wild-type (thyA+) Escherichia coli K-12. J. Bacteriol. 175: 909-911.
33. Low, B. 1968. Formation of merodiploids in matings with a class of rec- recipient strains of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 60: 160-168.
34. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
35. Matthews, R. 1996. One-carbon metabolism. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 600-611.
36. Messing, J. 1983. New M13 vectors for cloning. Methods Enzymol. 101: 20-78.
37. Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
38. Nagy, P.L., Marolewski, A., Benkovic, S.J., and Zalkin, H. 1995. Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli. J. Bacteriol. 177: 1292-1298.
39. Neuhard, J., and Kelln, R.A. 1996. Biosynthesis and conversion of pyrimidines. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 580-599.
40. Norrander, J., Kempe, T., and Messing, J. 1983. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene, 26: 101-106.
41. Oka, A., Sugisaki, H., and Takanami, M. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226.
42. Phillips, S.L., Schlessinger, D., and Apirion, D. 1969. Temperature-dependent suppression of UGA and UAA codons in a temperature-sensitive mutant of Escherichia coli. Cold Spring Harbor Symp. Quant. Biol. 34: 499-503.
43. Poe, M., Greenfield, N.J., Hirshfield, J.M., Williams, M.N., and Hoogsteen, K. 1972. Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli. Biochemistry, 11: 1023-1030.
44. Pyne, C., and Bognar, A.L. 1992. Replacement of the folC gene, encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli, with genes mutagenized in vitro. J. Bacteriol. 174: 1750-1759.
45. Rohlman, C.E., and Matthews, R.G. 1990. Role of purine biosynthetic intermediates in response to folate stress in Escherichia coli. J. Bacteriol. 172: 7200-7210.
46. Roodman, S.T., and Greenberg, G.R. 1971. A temperature sensitive thyA mutant blocked in the synthesis of thymidylate synthetase. J. Biol. Chem. 246: 2609-2617.
47. Russel, M., and Holmgren, A. 1988. Construction and characterization of glutaredoxin-negative mutants of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 85: 990-994.
48. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
49. Schirch, V., and Strong, W.B. 1989. Interaction of folylpolyglutamates with enzymes in one-carbon metabolism. Arch. Biochem. Biophys. 269: 371-380.
50. Singer, M., Baker, T.A., Schnitzler, G., Deischel, S.M., Goel, M., Dove, W., Jaacks, K.J., Grossman, A.D., Erickson, J.W., and Gross, C.A. 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53: 1-24.
51. Singer, S., Ferone, R., Walton, L., and Elwell, L. 1985. Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli. J. Bacteriol. 164: 470-472.
52. Smith, D.R., and Calvo, J.M. 1980. Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 8: 2255-2274.
53. Stauffer, G.V. 1996. Biosynthesis of serine, glycine and one-carbon units. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 506-513.
54. Taylor, L.A., and Rose, R.E. 1988. A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K. Nucleic Acids Res. 16: 358.
55. Vasudevan, S.G., Paal, B., and Armarego, W.L.F. 1992. Dihydropteridine reductase from Escherichia coli exhibits dihydrofolate reductase activity. Biol. Chem. Hoppe-Seyler, 373: 1067-1073.
56. Vieira, J., and Messing, J. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19: 259-268.
57. Villafranca, J.E., Howell, E.E., Voet, D.H., Strobel, M.S., Ogden, R.C., Abelson, J.N., and Kraut, J. 1983. Directed mutagenesis of dihydrofolate reductase. Science (Washington, D.C.), 222: 782-788.
58. Yanisch-Perron, C., Vieira, J., and Messing, J. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33: 103-119.
59. Zalkin, H., and Nygaard, P. 1996. Biosynthesis of purine nucleotides. In Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Edited by F.C. Neidhardt. ASM Press, Washington. pp. 561-599.