The structure of the llama heavy chain constant genes reveals a mechanism for heavy-chain antibody formation

Immunogenetics

Volumn 50, Issue 1-2, 1999, Pages 98-101

  Woolven, Benjamin P ^a   Frenken, Leon G J ^a   Van Der Logt, Paul ^a   Nicholls, Peter J ^b  

^a UNILEVER RESEARCH   (United Kingdom)

^b UNIVERSITY OF KENT   (United Kingdom)

Author keywords

Antibody; CH1; Llama; Mutation; Splice

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN;

ANTIBODY PRODUCTION; ARTICLE; GENE STRUCTURE; IMMUNOGLOBULIN GENE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES; BASE SEQUENCE; CAMELIDS, NEW WORLD; GENE LIBRARY; GENES, IMMUNOGLOBULIN; IMMUNOGLOBULIN CONSTANT REGIONS; IMMUNOGLOBULIN HEAVY CHAINS; MALE; MOLECULAR SEQUENCE DATA; RNA SPLICING; SEQUENCE HOMOLOGY, NUCLEIC ACID;

LAMA GLAMA;

EID: 0032841561     PISSN: 00937711     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002510050694     Document Type: Article

References (9)

1. Aebi M, Hornig H, Weissmann C (1987) 5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice region, not by the conserved 5′ GU. Cell 50:237-246
2. Brunak S, Engelbrecht J, Knudsen S (1991) Prediction of human mRNA donor and acceptor sites from the DNA sequence. J Mol Biol 220:49-65
3. Davies J, Riechmann L (1996) Affinity improvement of single antibody VH domains: residues in all three hypervariable regions affect antigen binding. Immunotechnology 2:169-179
4. Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L (1996) Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol 3:803-811
5. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB, Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
6. Honjo T, Matsuda F (1995) Immunoglobulin heavy chain loci of mouse and human. In: Honjo T, Alt FW (eds) Immunoglobulin genes. Academic Press, London, pp145-171
7. Nguyen VK, Muyldermans S, Hamers R (1998) The specific variable domain of camel heavy-chain antibodies is encoded in the germline. J Mol Biol 275:413-418
8. Spinelli S, Frenken L, Bourgeois D, de Ron L, Bos W, Verrips T, Anguille C, Cambillau C, Tegoni M (1996) The crystal structure of a llama heavy chain variable domain. Nat Struct Biol 3:752-757
9. H sequences from conventional and heavy chain antibodies. Mol Immunol 34:1121-31