Direct sequencing of the PCR amplified SSU rRNA gene of Entamoeba dispar and the design of primers for rapid differentiation from Entamoeba histolytica

Parasitology

Volumn 112, Issue 4, 1996, Pages 363-369

  Novati, S ^a   Sironi, M ^b   Granata, S ^a   Bruno, A ^a   Gatti, S ^a   Scaglia, M ^a   Bandi, C ^a,b  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

Direct sequencing; Entamoeba dispar; Entamoeba histolytica; RFLP; Ribosomal RNA; Species identification

Indexed keywords

RIBOSOME RNA;

AMEBIASIS; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENTAMOEBA; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECIES DIFFERENTIATION;

EID: 0029965420     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/s0031182000066592     Document Type: Article

References (43)

1. ACUNA-SOTO, R., SAMUELSON, J., DE GIROLAMI, P., ZARATE, L., MILLAN-VELASCO, F., SCHOOLNICK, G. & WIRTH, D. (1993). Application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic Entamoeba histolytica. American Journal of Tropical Medicine and Hygiene 48, 58-70.
2. ANDREWS, B. J., MENTZONI, L. & BJORVATN, B. (1990). Zymodeme conversion of isolates of Entamoeba histolytica. Transactions of the Royal Society of Tropical Medicine and Hygiene 84, 63-5.
3. BANDI, C., DAMIANI, G., MAGRASSI, L., GRIGOLO, A., FANI, R. & SACCHI, L. (1994). Flavobacteria as intracellular symbionts in cockroaches. Proceedings of the Royal Society of London, B 257, 43-8.
4. BHATTACHARYA, S., BHATTACHARYA, A. & DIAMOND, L. S. (1988). Comparison of repeated DNA from strains of Entamoeba histolytica and other Entamoeba. Molecular and Biochemical Parasitology 27, 257-62.
5. BHATTACHARYA, S., BHATTACHARYA, A., DIAMOND, L. S. & SOLDO, A. T. (1989). Circular DNA of Entamoeba histolytica encodes ribosomal RNA. Journal of Protozoology 36, 455-8.
6. BLANC, D. & SARGEAUNT, P. G. (1991). Entamoeba histolytica zymodemes: exhibition of γ and δ bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium. Experimental Parasitology 72, 87-90.
7. BRUMPT, E. (1925). Etude sommarie de l' 'Entamoeba dispar' n. sp. Amibe à kystes quadrinuclées, parasite de l'homme. Bulletin de l'Académie de Médecine (Paris) 94, 943-52.
8. CLARK, C. G., CUNNICK, C. C. & DIAMOND, L. S. (1992). Entamoeba histolytica: is conversion of 'nonpathogenic' amebae to the 'pathogenic' form a real phenomenon? Experimental Parasitology 74, 307-14.
9. CLARK, C. G. & DIAMOND, L. S. (1991). Ribosomal RNA genes of 'pathogenic' and 'nonpathogenic' Entamoeba histolytica are distinct. Molecular and Biochemical Partisitology 49, 297-302.
10. CLARK, C. G. & DIAMOND, L. S. (1993). Entamoeba histolytica: an explanation for the reported conversion of nonpathogenic amoebae to the pathogenic form. Experimental Parasitology 77, 456-60.
11. CRUZ-REYES, J. A., SPICE, W. M., REHMAN, T., GISBORNE, E. & ACKERS, J. P. (1992). Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica. Parasitology 104, 239-46.
12. DIAMOND, L. S. & CLARK, G. (1993). A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. Journal of Eurkaryotic Microbiology 40, 340-4.
13. DOBSON, S. J., COLWELL, K. K., MCMEEKIN, T. A. & KRANZMANN, P. D. (1993). Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from hypersaline antartic lake. International Journal of Systematic Bacteriology 43, 77-83.
14. GATTI, S., CEVINI, C., MARCHI, L., NOVATI, S. & SCAGLIA, M. (1995). Entamoeba histolytica autochthonous isolates from mentally retarded Italian patients. Parasitology Research 81, 148-51.
15. HILLIS, D. M., LARSON, A., DAVIS, S. K. & ZIMMER, E. A. (1990). Nucleic acids III: sequencing. In Molecular Systematics (ed. Hillis, D. M. & Moritz, C.), pp. 318-70. Sunderland, Massachusetts: Sinauer Associates.
16. HUBER, M., KOLLER, B., GITLER, C., MIRELMAN, D., REVEL, M., ROZENBLATT, S. & GARFINKEL, L. (1989). Entamoeba histolytica ribosomal RNA genes are carried on palindromic circular DNA molecules. Molecular and Biochemical Parasitology 32, 285-96.
17. JACKSON, T. F. H. G., GATHIRAM, V., SUPARSAD, S. & ANDERSON, C. A. (1992). Stability of the zymodemes of Entamoeba histolytica in cultures. Archives of Medical Research 23, 71.
18. KATZWINKEL-WLADARSCH, S., LÖSCHER, T. & RINDER, H. (1994). Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens. American Journal of Tropical Medicine and Hygiene 51, 115-18.
19. LEIPE, D. D., GUNDERSON, J. H., NERAD, T. A. & SOGIN, M. L. (1993). Small subunit ribosomal RNA+ of Hexamita inflata and the quest for the first branch in the eukariotic tree. Molecular and Biochemical Parasitology 59, 41-8.
20. MAIDAK, B. L., LARSEN, N., McCAUGHEY, M. J., OVERBEEK, R., OLSEN, G. J., FOGEL, K., BLANDY, J. WOESE, C. R. (1994). The Ribosomal Database project. Nucleic Acids Research 22 (Suppl.), 3485-7.
21. MARTINEZ-PALOMO, A., MARTINEZ-ROBLES, A. & DE LA TORRE, M. (1973). Selective agglutination of pathogenic strains of Entamoeba histolytica induced con A. Nature, New Biology 245, 186-7.
22. MIRELMAN, D., BRACHA, R., CHAYEN, A., AUST-KETTIS, A. & DIAMOND, L. S. (1986a). Entamoeba histolytica: effect of growth conditions and bacterial associates on isoenzyme patterns and virulence. Experimental Parasitology 62, 142-8.
23. MIRELMAN, D., BRACHA, R., WEXLER, A. & CHAYEN, A. (1986b). Changes in isoenzyme patterns of a cloned culture of non-pathogenic Entamoeba histolytica during axenization. Infection and Immunity 54, 827-32.
24. MUKHERJEE, R. M., BHOL, K. C., MEHRA, S., MAITRA, T. K. & JALAN, K. N. (1993). Zymodeme alteration of Entamoeba histolytica isolates under varying conditions. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 490-1.
25. OCHMAN, H. & WILSON, A. C. (1987). Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. Journal of Molecular Evolution 26, 74-86.
26. QUE, X. & REED, S. L. (1991). Nucleotide sequence of a small subunit ribosomal RNA (16S-like rRNA) gene from Entamoeba histolytica: differentiation of pathogenic from nonpathogenic isolates. Nucleic Acids Research 19, 5438.
27. RAMACHANDRAN, S., BHATTACHARYA, A. & BHATTACHARYA, S. (1993). Nucleotide sequence analysis of the rRNA transcription unit of a pathogenic Entamoeba histolytica strain HM-1: IMSS. Nucleic Acids Research 21, 2011.
28. ROBINSON, G. L. (1968). The laboratory diagnosis of human parasitic amoebae. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 285-94.
29. SAMBROOK, J., FRITSCH, E. F. & MANIATIS, T. (1989). Molecular Cloning: a Laboratory Manual. 2nd Edn. Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press.
30. SARGEAUNT, P. G. (1988). Zymodemes of Entamoeba histolytica. In Amoebiasis. Human Infection by Entamoeba histolytica (ed. Ravdin, J. I.), pp. 370-421. New York: Churchill Livingstone Publications.
31. SARGEAUNT, P. G. (1992). Entamoeba histolytica is a complex of two species. Australian Microbiologist 13, 359-61.
32. SARGEAUNT, P. G. (1994). Entamoeba histolytica and E. dispar. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 254.
33. SARGEAUNT, P. G. & WILLIAMS, J. E. (1978). Electrophoretic isoenzyme patterns of Entamoeba histolytica and Entamoeba coli. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 164-6.
34. SARGEAUNT, P. G., WILLIAMS, J. E. & GRENE, J. D. (1978). The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 519-21.
35. SARGEAUNT, P. G., JACKSON, T. F. H. G., WIFFEN, S. R. & BHOJNANI, R. (1988). Biological evidence of genetic exchange in Entamoeba histolytica. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 862-7.
36. SCAGLIA, M., GATTI, S., BRUNO, A., CEVINI, C., MARCHI, L. & SARGEAUNT, P. G. (1991). Autochthonous amoebiasis in institutionalized mentally-retarded patients: preliminary evaluation of isoenzyme patterns in three isolates. Annals of Tropical Medicine and Parasitology 85, 509-13.
37. SCHLÖTTERER, C., HAUSER, M.-T., VON HAESELER, A. & TAUTZ, D. (1994). Comparative evolutionary analysis of rDNA ITS region in Drosophila. Molecular Biology and Evolution 11, 513-22.
38. SEHGAL, D., MITTAL, V., RAMACHANDRAN, S., DHAR, S. K., BHATTACHARYA, A. & BHATTACHARYA, S. (1994). Nucleotide sequence organisation and analysis of the nuclear ribosomal DNA circle of the protozoan parasite Entamoeba histolytica. Molecular and Biochemical Parasitology 67, 205-14.
39. SIDOW, A. & THOMAS, W. K. (1994). A molecular evolutionary framework for eukaryotic model organisms. Current Biology 4, 596-603.
40. SOGIN, M. L. (1989). Evolution of eukaryotic microorganisms and their small subunit ribosomal RNAs. American Zoologist 29, 487-99.
41. TANNICH, E. & BURCHARD, G. D. (1991). Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro. Journal of Clinical Microbiology 29, 250-5.
42. VARGAS, M. A. & OROZCO, E. (1993). Entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic trophozoites cultured under different conditions. Parasitology Research 79, 353-6.
43. WALSH, J. A. (1986). Problems in recognition and diagnosis of amoebiasis: estimation of the global magnitude of morbidity and mortality. Reviews of Infectious Diseases 8, 228-38.