Rapid high-performance liquid chromatographic method for determination of debrisoquine and 4-hydroxydebrisoquine in urine for CYP2D6 phenotyping

Pharmacogenetics

Volumn 9, Issue 4, 1999, Pages 529-531

  Svensson, Jan Olof ^a   Bertilsson, Leif ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

CYP2D6; Debrisoquine; Phenotype

Indexed keywords

4 HYDROXYDEBRISOQUINE; CYTOCHROME P450 ISOENZYME; DEBRISOQUINE;

ARTICLE; DRUG HYDROXYLATION; DRUG METABOLISM; DRUG URINE LEVEL; GAS CHROMATOGRAPHY; GENE AMPLIFICATION; GENE DUPLICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; PHARMACOGENETICS; PHENOTYPE; PRIORITY JOURNAL;

EID: 0032822399     PISSN: 0960314X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Bertilsson L, Dahl M-L. Polymorphic drug oxidation. Relevance to the treatment of psychiatric disorders. CNS Drugs 1996; 5:200-223.
2. Bozkurt A, Basci N, Isimer A, Kayaalp S. Determination of debrisoquine and 4-hydroxydebrisoquine in urine by high-performance liquid chromatography with fluorescence detection after solid-phase extraction. J Pharm Biomed Anal 1993; 11:745-749.
3. Frye R, Branch R. Improved high-performance liquid chromatographic determination of debrisoquine and 4-hydroxydebrisoquine in human urine following direct injection. J Chromatogr B 1996; 677:178-182.
4. Hempenius J, Staal B, Jeuring HJ, Mensink CK, Jonkman JHG. Determination of debrisoquine and 4-hydroxydebrisoquine in human urine by high performance liquid chromatography and fluorescence detection. J Liquid Chromatogr 1990; 138:1627-1636.
5. Johansson I, Lundqvist E, Bertilsson L, Dahl M-L, Sjöqvist F, Ingelman-Sundberg I. Inherited amplification of an active gene in the cytochrome P450 CYP2D-locus as a cause of ultrarapid metabolism of debrisoquine. Proc Natl Acad Sci USA 1993; 90:11825-11829.
6. Lennard MS, Silas JH, Smith AJ, Tucker GT. Determination of debrisoquine and its 4-hydroxymetabolite in biological fluids by gas chromatography with flame-ionization and nitrogen -selective detection. J Chromatogr 1977; 133:161-168.
7. Mahgoub A, Idle JR. Dring LG, Lancaster R, Smith RL. Polymorphic hydroxylation of debrisoquine in man. Lancet 1977; 2:584-586.
8. Tucker GT, Silas JH, Iyun AO, Lennard MS, Smith AJ. Polymorphic hydroxylation of debrisoquine in man. Lancet 1977; 2:718.
9. Westwood BE, Harman J, Mashford M L. Liquid chromatographic assay for debrisoquine and 4-hydroxydebrisoquine in urine. J Chromatogr 1986; 374:200-203.