Quantitative measurement of mammalian chromosome mitotic loss rates using the green fluorescent protein

Journal of Cell Science

Volumn 112, Issue 16, 1999, Pages 2705-2714

  Burns, Elizabeth M ^a   Christopoulou, Lina ^a   Corish, Pete ^a   Tyler Smith, Chris ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Aneuploidy; Chromosome loss; GFP; Mammalian chromosome transmission assay

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIVISION; CHROMOSOME LOSS; FLUORESCENCE ACTIVATED CELL SORTER; HELA CELL; HUMAN; HUMAN CELL; MAMMAL; MITOSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY;

ANIMALIA; MAMMALIA;

EID: 0032819855     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Cahill, D. P., Lengauer, C., Yu, J., Riggins, G. J., Willson, J. K. V., Markowitz, S. D., Kinzler, K. W. and Vogelstein, B. (1998). Mutations of mitotic checkpoint genes in human cancers. Nature 392, 300-303.
2. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W. and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
3. Cohen, D., Chumakov, I. and Weissenbach, J. (1993). A first-generation physical map of the human genome. Nature 366, 698-701.
4. Featherstone, T. and Huxley, C. (1993). Extrachromosomal maintenance and amplification of yeast artificial chromosome DNA in mouse cells. Genomics 17, 267-278.
5. Gietz, D., St Jean, A., Woods, R. A. and Schiestl, R. H. (1992). Improved method for high efficiency transformation of intact yeast cells. Nuceic. Acids Res. 20,1425.
6. Griffin, D. K. (1996). The incidence, origin and etiology of aneuploidy. Int. Rev. Cytol. 167, 263-296.
7. Guttenbach, M., Korschorz, B., Bernthaler, U., Grimm, T. and Schmid, M. (1995). Sex chromosome loss and aging: in situ hybridisation studies on human interphase nuclei. Am. J. Hum. Genet. 57, 1143-1150.
8. Heim, R., Cubitt, A. B. and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
9. Hieter, P., Mann, C., Snyder, M. and Davis, R. W. (1985). Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell 40, 381-392.
10. Hugerat, Y., Spencer, F., Zenvirth, D. and Simchen, G. (1994). A versatile method for efficient YAC transfer between any two strains. Genomics 22, 108-117.
11. Jin, D., Spencer, F. and Jeang, K. (1998). Human T cell leukemia virus type 1 oncoprotein tax targets the human mitotic checkpoint protein MAD1. Cell 93, 81-91.
12. Jordan, M. A., Thrower, D. and Wilson, L. (1992). Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. J. Cell Sci. 102, 401-416.
13. Kirsch-Vodlers, M., Elhajouji, A., Cundari, E. and Hummelen, P. V. (1997). The in vitro micronucleus test: a multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutn. Res. 392, 19-30.
14. Kitazono, A and Matsumoto, T. (1998). 'Isogaba maware': quality controls of genome DNA by checkpoints. BioEssays 20, 391-399.
15. Koshland, D., Kent, J. C. and Hartwell, L. H. (1985). Genetic analysis of the mitotic transmission of minichromosomes. Cell 40, 393-403.
16. Lau, C. C. and Pardee, A. B. (1982). Mechanism by which caffeine potentiates lethality of nitrogen mustard. Proc. Natt. Acad. Sci. USA 79, 2942-2946.
17. Lengauer, C., Kinzler, K. W. and Vogelstein, B. (1998). Genetic instabilities in human cancers. Nature 396, 643-649.
18. Li, R., Terganian, G., Duesberg, P., Kraemer, A., Willer, A., Rausch, C. and Hehlmann, R. (1997). Aneuploidy correlated 100% with chemical transformation of Chinese hamster cells. Proc. Natl. Acad. Sci. USA 94, 14506-14511.
19. Mitelman, F. (1994). Catalog of Chromosome Aberrations in Cancer. Wiley-Liss, NY.
20. Murphy, T. D. and Karpen, G. H. (1998). Centromeres take flight: alpha satellite and the quest for the human centromere. Cell 93, 317-320.
21. Niwa, H., Yamamura, K. and Miyazaki, J. (1991). Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193-200.
22. Page, D. C., Harper, M. E. Love, J. and Botstein, D. (1984). Occurrence of a transposition from the X-chromosome long arm to the Y-chromosome short arm during human evolution. Nature 311, 119-123.
23. Parry, E. M., Henderson, L. and Mackay, J. M. (1995). Procedures for the detection of chemically induced aneuploidy: recommendations of a UK Environmental Mutagen Society working group. Mutagenesis 10,1-14.
24. Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G. and Earnshaw, W. C. (1995). The centromere: hub of chromosomal activities. Science 270, 1591-1594.
25. Rose, M. D., Winston, F. and Hieter, P. (1990). Methods in Yeast Genetics, A Laboratory Course Manual. Cold Spring Harbor Press, Cold Spring Harbor.
26. Sandhu, S. S., Gudi, R. and Athwal, R. S. (1988). A genetic assay for aneuploidy: quantitation of chromosome loss using a mouse/human monochromosomal hybrid cell line. Mut. Res. 201. 423-430.
27. Shen, M. H., Yang, J., Loupart, M.-L., Smith, A. and Brown, W. (1997). Human mini-chromosomes in mouse embryonal stem cells. Hum. Mol. Genet. 6, 1375-1382.
28. Southern, E. M., Anand, R., Brown, W. R. A. and Fletcher, D. S. (1987). A model for the separation of large DNA molecules by crossed field gel electrophoresis. Nucleic Acids Res. 15, 5925-5943.
29. Straight, A. F. (1997). Cell cycle: checkpoint proteins and kinetochores. Curr. Biol. 7, R613-R616.
30. Sullivan, K. F. and Glass, C. A. (1991). CENP-B is a conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. Chromosoma 100, 360-370.
31. Taylor, S. S., Larin, Z. and Tyler-Smith, C. (1996). Analysis of extrachromosomal structures containing human centromeric alphoid satellite DNA sequences in mouse cells. Chromosoma 105, 70-81.
32. Vasquez, R. J., Howqell, B., Yvon, A. C., Wads worth, P. and Cassimeris, L. (1997). Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol. Biol. Cell. 8, 973-985.
33. Vassetzky, Y. S., Alghisi, G. and Gasser, S. M. (1995). DNA topoisomerase II mutations and resistance to anti-tumor drugs. BioEssays 17, 767-774.