The role of the 7B2 CT peptide in the inhibition of prohormone convertase 2 in endocrine cell lines

Journal of Neurochemistry

Volumn 73, Issue 3, 1999, Pages 994-1003

  Fortenberry, Yolanda ^a   Liu, June ^a   Lindberg, Iris ^a  

^a LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

Author keywords

Carboxyl terminal peptide; Proenkephalin; Prohormone convertase 2

Indexed keywords

ALPHA INTERMEDIN; FURIN; NEUROPEPTIDE; PROENKEPHALIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENDOCRINE CELL; ENZYME INHIBITION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PROCESSING; PROTEIN SECRETION; RAT;

ANIMALS; CELL LINE; CHO CELLS; CHROMATOGRAPHY, GEL; CRICETINAE; CYTOPLASMIC GRANULES; ENDOCRINE GLANDS; ENKEPHALINS; MICE; NERVE TISSUE PROTEINS; NEUROENDOCRINE SECRETORY PROTEIN 7B2; PC12 CELLS; PITUITARY HORMONES; PRECIPITIN TESTS; PRO-OPIOMELANOCORTIN; PROPROTEIN CONVERTASE 2; PROTEIN PRECURSORS; RADIOIMMUNOASSAY; RATS; RECOMBINANT FUSION PROTEINS; SUBTILISINS; TRANSFECTION;

EID: 0032819395     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1999.0730994.x     Document Type: Article

References (41)

1. 1-antitrypsin variant. J. Biol. Chem. 268, 24887-24891.
2. Anderson E. D., VanSlyke J. K., Thulin C. D., Jean F., and Thomas G. (1997) Activation of the furin endoprotease is a multiple-step process: requirements for acidification and internal propeptide cleavage. EMBO J. 16, 1508-1518.
3. Apletalina E., Appel J., Lamango N. S., Houghten R. A., and Lindberg I. (1998) Identification of inhibitors of prohormone convertases 1 and 2 using a peptide combinatorial library. J. Biol. Chem. 273, 26589-26595.
4. Ayoubi T. A. Y., van Duijnhoven H. L. P., van de Ven W. J. M., Jenks B. G., Roubos E. W., and Martens G. J. M. (1990) The neuroendocrine polypeptide 7B2 is a precursor protein. J. Biol. Chem. 265, 15644-15647.
5. Benjannet S., Rondeau N., Paquet L., Boudreault A., Lazure C., Chretien M., and Seidah N. G. (1993) Comparative biosynthesis, covalent post-translational modifications and efficiency of prosegment cleavage of the prohormone convertases PC1 and PC2: glycosylation, sulphation and identification of the intracellular site of prosegment cleavage of PC1 and PC2. Biochem. J. 294, 735-743.
6. Benjannet S., Savaria D., Laslop A., Munzer J. S., Chretien M., Marcinkiewicz M., and Seidah N. G. (1997) Alpha 1-antitrypsin Portland inhibits processing of precursors mediated by proprotein convertases primarily within the constitutive secretory pathway. J. Biol. Chem. 272, 26210-26218.
7. Breslin M. B., Lindberg I., Benjannet S., Mathis J. P., Lazure C., and Seidah N. G. (1993) Differential processing of proenkephalin by prohormone convertases 1(3) and 2 and furin. J. Biol. Chem. 268, 27084-27093.
8. Day R., Schafer M. K. H., Watson S. J., Chretien M., and Seidah N. G. (1992) Distribution and regulation of the prohormone convertases PC1 and PC2 in the rat pituitary. Mol. Endocrinol. 6, 485-497.
9. Guest P. C., Arden S. D., Bennett D. L., Clark A., Rutherford N. G., and Hutton J. C. (1992) The post-translational processing and intracellular sorting of PC2 in the islets of Langerhans. J. Biol. Chem. 267, 22401-22406.
10. Hatsuzawa K., Hosaka M., Nakagawa T., Nagase M., Shoda A., Murakami K., and Nakayama K. (1990) Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells. J. Biol. Chem. 265, 22075-22078.
11. Johanning K., Mathis J. P., and Lindberg I. (1996a) Processing site blockade results in more efficient conversion of proenkephalin to active opioid peptides. J. Biol. Chem. 271, 27871-27878.
12. Johanning K., Mathis J. P., and Lindberg I. (1996b) Role of PC2 in proenkephalin processing: antisense and overexpression studies. J. Neurochem. 66, 898-907.
13. Johanning K., Juliano M. A., Juliano L., Lazure C., Lamango N., Steiner D. F., and Lindberg I. (1998) Specificity of prohormone convertase 2 on proenkephalin and proenkephalin-related fluorogenic peptides. J. Biol. Chem. 273, 22672-22680.
14. Kiefer M. C., Tucker J. E., Joh R., Landsberg K. E., Saltman D., and Barr P. J. (1991) Identification of a second human subtilisin-like protease gene in the fes/fps region of chromosome 15. DNA Cell Biol. 10, 757-769.
15. Lamango N. S., Zhu X., and Lindberg I. (1996) Purification and enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2. Arch. Biochem. Biophys. 330, 238-250.
16. Lamango N. S., Apletalina E., Liu J., and Lindberg I. (1999) The proteolytic maturation of prohormone convertase 2 (PC2) is a pH-driven process. Arch. Biochem. Biophys. 362, 275-282.
17. Lindberg I. (1986) Reserpine-induced alterations in the processing of proenkephalin in cultured chromaffin cells: increased amidation. J. Biol. Chem. 261, 16317-16323.
18. Lindberg I. (1994) Evidence for the cleavage of the PC1/PC3 prosegment in the endoplasmic reticulum. Mol. Cell. Neurosci. 5, 263-268.
19. Lindberg I. and White L. (1986) Reptilian enkephalins: implication for the evolution of proenkephalin. Arch. Biochem. Biophys. 245, 1-7.
20. Lindberg I. and Zhou Y. (1995) Overexpression of neuropeptide precursor and processing enzymes, in Peptidases and Neuropeptide Processing (Smith A. I., ed), pp. 94-108. Academic Press, San Diego.
21. Lindberg I., Shaw E., Finley J., Leone D., and Deininger P. (1991) Posttranslational modifications of recombinant rat proenkephalin overexpressed in Chinese hamster ovary cells. Endocrinology 128, 1849-1856.
22. Lindberg I., Ahn S., and Breslin M. (1994) Cellular distributions of the prohormone processing enzymes PC1 and PC2. Mol. Neurosci. 5, 614-622.
23. Lindberg I., Van den Hurk W. H., Bui C., and Batie C. J. (1995) Enzymatic characterization of immunopurified prohormone convertase 2. Potent inhibition by a 7B2 peptide fragment. Biochemistry 34, 5486-5493.
24. Mains R. E., Dickerson I. M., May V., Stoffers D. A., Perkins S. N., Ouafik L., Husten E. J., and Eipper B. (1990) Cellular and molecular aspects of peptide hormone biosynthesis. Front. Neuroendocrinol. 11, 52-89.
25. Martens G. J. M., Braks J. A. M., Eib D. W., Zhou Y., and Lindberg I. (1994) The neuroendocrine polypeptide 7B2 is an endogenous inhibitor of prohormone convertase PC2. Proc. Natl. Acad. Sci. USA 91, 5784-5785.
26. Mathis J. and Lindberg I. (1992) Posttranslational processing of proenkephalin in AtT-20 cells: evidence for cleavage at a Lys-Lys site. Endocrinology 131, 2287-2296.
27. Milgram S. L. and Mains R. E. (1994) Differential effects of temperature blockade on the proteolytic processing of three secretory granule-associated proteins. J. Cell Sci. 107, 737-745.
28. Paquet L., Bergeron F., Boudreault A., Seidah N. G., Chretien M., Mbikay M., and Lazure C. (1994) The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furinlike convertase. J. Biol. Chem. 269, 19279-19285.
29. Rouille Y., Duguay S. J., Lund K., Furuta M., Gong Q., Lipkind G., Oliva A. A. Jr., Chan S. J., and Steiner D. F. (1995) Proteolytic processing mechanisms in the biosynthesis of neuroendocrine peptides: the subtilisin-like proprotein convertases. Front. Neuroendocrinol. 16, 322-361.
30. Seidah N. G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., and Chretien M. (1990) cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases. DNA Cell Biol. 9, 415-424.
31. Seidah N. G., Marcinkiewicz M., Benjannet S., Gaspar L., Beaubien G., Mattei M. G., Lazure C., Mbikay M., and Chretien M. (1991) Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2. Mol. Endocrinol. 5, 111-122.
32. Shen F.-S., Lindberg I., and Seidah N. G. (1993) Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells. J. Biol. Chem. 268, 24910-24915.
33. Smeekens S. P., Avruch A. S., LaMendola J., Chan S. J., and Steiner D. F. (1991) Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans. Proc. Natl. Acad. Sci. USA 88, 340-344.
34. Steiner D. F. (1998) The proprotein convertases. Curr. Opin. Chem. Biol. 2, 31-39.
35. Westphal C. H., Muller L., Zhou A., Bonner-Weir S., Schambelan M., Steiner D. F., Lindberg I., and Leder P. (1999) The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease. Cell 96, 689-700.
36. Zhou A. and Mains R. E. (1994) Endoproteolytic processing of POMC and prohormone convertases 1 and 2 in neuroendocrine cells overexpressing PC1 and PC2. J. Biol. Chem. 269, 17440-17447.
37. Zhou A., Bloomquist B. T., and Mains R. E. (1993) The prohormone convertases PC1 and PC2 mediate distinct endoproteolytic cleavages in a strict temporal order during proopiomelanocortin biosynthetic processing. J. Biol. Chem. 268, 1763-1769.
38. Zhou Y. and Lindberg I. (1993) Purification and characterization of the prohormone convertase PC1(PC3). J. Biol. Chem. 268, 5615-5623.
39. Zhu X. and Lindberg I. (1995) 7B2 facilitates the maturation of proPC2 in neuroendocrine cells and is required for the expression of enzymatic activity. J. Cell Biol. 129, 1641-1650.
40. Zhu X., Lamango N. S., and Lindberg I. (1996a) Involvement of a polyproline helix-like structure in the interaction of 7B2 with prohormone convertase 2. J. Biol. Chem. 271, 23582-23587.
41. Zhu X., Rouille Y., Lamango N. S., Steiner D. F., and Lindberg I. (1996b) Internal cleavage of the inhibitory 7B2 carboxyl-terminal peptide by PC2: a potential mechanism for its inactivation. Proc. Natl. Acad. Sci. USA 93, 4919-4924.