Expression of hydrophilic surface proteins in infective stages of Leishmania donovani

Molecular and Biochemical Parasitology

Volumn 102, Issue 1, 1999, Pages 191-196

  Alce, Timothy M ^a   Gokool, Suzanne ^a   McGhie, Diane ^a   Stäger, Simona ^a   Smith, Deborah F ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Amino acid repeats; Hydrophilic acylated surface proteins; Infective stage antigens; Leishmania donovani

Indexed keywords

MEMBRANE PROTEIN; PARASITE ANTIGEN; REPETITIVE DNA;

ANIMAL EXPERIMENT; ANTIGEN EXPRESSION; CONTROLLED STUDY; HYDROPHILICITY; IMMUNOBLOTTING; LEISHMANIA DONOVANI; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SHORT SURVEY;

ANIMALIA; LEISHMANIA DONOVANI;

EID: 0032809933     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00074-2     Document Type: Article

References (15)

1. Flinn H.M., Rangarajan D., Smith D.F. Expression of a hydrophilic surface protein in infective stages of Leishmania major. Mol. Biochem. Parasitol. 65:1994;259-270.
2. Pimenta P.F.P., da Silva P.P., Rangarajan D., Smith D.F., Sacks D.L. Leishmania major: association of the differentially expressed gene B protein and the surface lipophosphoglycan as revealed by membrane capping. Exp. Parasitol. 79:1994;468-479.
3. Rangarajan D., Gokool S., McCrossan M.V., Smith D.F. The gene B protein localises to the surface of Leishmania major parasites in the absence of metacyclic stage lipophosphoglycan. J. Cell. Sci. 108:1995;3359-3366.
4. McKean P.G., Delahay R., Pimenta P.F.P., Smith D.F. Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major. Mol. Biochem. Parasitol. 85:1997;221-232.
5. Guss B., Uhlen M., Nilsson B., Lindberg M., Sjoquist J., Sjodahl J. Region X, the cell-wall-attachment part of staphylococcal protein A. Eur. J. Biochem. 138:1984;413-420.
6. McKean P.G., Trenholme K.R., Rangarajan D., Keen J., Smith D.F. Diversity in repeat-containing surface proteins of Leishmania major. Mol. Biochem. Parasitol. 86:1997;225-235.
7. Smith R.F., Smith T.F. Pattern-Induced Multi-Sequence Alignment (PIMA) algorithm employing secondary structure-dependent gap penalties for comparative protein modelling. Protein Eng. 5:1992;35-41.
8. Lohman K.L., Langer P.J., McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proc. Natl. Acad. Sci. USA. 87:1990;8393-8397.
9. Wallis A.E., Russell D.G., McMaster W.R. Leishmania major: organization and conservation of genes encoding repetitive peptides and subcellular localisation of the corresponding proteins. Exp. Parasitol. 78:1994;161-174.
10. Symons F.M., Murray P.J., Ji H., Simpson R.J., Osborn A.H., Cappai R., Handman E. Characterization of a polymorphic family of integral membrane proteins in promastigotes of different Leishmania species. Mol. Biochem. Parasitol. 67:1994;103-113.
11. Zhang W.W., Charest H., Ghedin E., Matlashewski G. Identification and over-expression of the A2 amastigote-specific protein in Leishmania donovani. Mol. Biochem. Parasitol. 78:1996;79-90.
12. Handman E., Symons F.M., Baldwin T.M., Curtis J.M., Scheerlinck J.P. Protective vaccination with promastigote surface antigen 2 from Leishmania major is mediated by a TH1 type of immune response. Infect. Immun. 63:1995;4261-4267.
13. Ghedin E., Zhang W.W., Charest H., Sundar S., Kenney R.T., Matlashewski G. Antibody response against a Leishmania donovani amastigote-stage-specific protein in patients with visceral leishmaniasis. Clin. Diag. Lab. Immunol. 4:1997;530-535.
14. Kemp M., Handman E., Kemp K., Ismail A., Mustafa M.D., Kordofani A.Y., Bendtzen K., Kharazmi A., Theander T.G. The Leishmania promastigote surface antigen-2 (PSA-2) is specifically recognised by Th1 cells in humans with naturally acquired immunity to L. major. FEMS Immunol. Med. Microbiol. 20:1998;209-218.
15. Jensen ATR, Gasim S, Moller T, Ismail A, Gaafar A, Kemp M, El Hassan AM, Kharazmi A, Alce TM, Smith DF, Theander TG. Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP. Trans R Soc Trop Med Hyg 1999; (in press).