Cloning and characterization of retinol dehydrogenase transcripts expressed in human epidermal keratinocytes

Molecular Genetics and Metabolism

Volumn 67, Issue 1, 1999, Pages 62-73

  Jurukovski, Vladimir ^a   Markova, Nedialka G ^a   Karaman Jurukovska, Nevena ^a   Randolph, R Keith ^a   Su, Jian ^b   Napoli, Joseph L ^b   Simon, Marcia ^a,c  

^a STONY BROOK UNIVERSITY   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c UNIVERSITY AT BUFFALO   (United States)

Author keywords

Epidermal keratinocytes; Retinol dehydrogenase; Retinol metabolism; Vitamin A

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE; RETINOIC ACID; RETINOL; RETINOL DEHYDROGENASE; STEROID;

ARTICLE; ENZYME SUBSTRATE; EPIDERMIS; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; KERATINOCYTE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0032802396     PISSN: 10967192     EISSN: None     Source Type: Journal    
DOI: 10.1006/mgme.1999.2840     Document Type: Article

References (49)

1. Fuchs E. Epidermal differentiation: The bare essentials. J Cell Biol. 111:1990;2807-2814.
2. Fuchs E, Byrne C. The epidermis: Rising to the surface. Curr Opin Genet Dev. 4:1994;725-736.
3. Saitou M, Sugai S, Tanaka T, Shimouchi K, Fuchs E, Narumiya, S, Kakizuka A. Inhibition of skin development by targeted expression of a dominant-negative retinoic acid receptor. Nature (London). 374:1995;159-162.
4. Imakado S, Bickenbach J R, Bundman D S, Rothnagel J A, Attar P S, Wang X-J, Walczak V R, Wisniewski S. Targeting expression of a dominant-negative retinoic acid receptor mutant in the epidermis of transgenic mice results in loss of barrier function. Genes Dev. 9:1995;317-329.
5. Fisher G J, Voorhees J J. Molecular mechanisms of retinoid action in skin. FASEB J. 10:1996;1002-1013.
6. Asselineau D, Bernard B A, Bailly C, Darmon M. Retinoic acid improves epidermal morphogenesis. Dev Biol. 133:1989;322-335.
7. Kang S, Duell E A, Fisher G J, Datta S C, Wang Z Q, Reddy A P, Tavakkol A, Jong Y Y. Application of retinol to human skin in vivo induces epidermal hyperplasia and cellular retinoid binding proteins characteristic of retinoic acid but without measurable retinoic acid levels or irritation. J Invest Dermatol. 105:1995;549-556.
8. Duell E A, Kang S, Voorhees J J. Retinoic acid isomers applied to human skin in vivo induce a 4-hydroxilase that inactivates only all trans retinoic acid. J Invest Dermatol. 106:1996;316-320.
9. Randolph, R, K, Siegenthaler, G, Vitamin A homeostasis in human epidermis: Native retinoid composition and metabolism, Handbook of Experimental Pharmacology, Retinoids, Berlin, Springer-Verlag.
10. Randolph R K, Simon M. Dermal fibroblasts actively metabolize retinoic acid but not retinol. J Invest Dermatol. 111:1998;478-484.
11. Napoli J L. Retinol metabolism in LLC-PK1 cells. Characterization of retinoic acid synthesis by an established mammalian cell line. J Biol Chem. 261:1986;13592-13597.
12. Chai X, Boerman M HEM, Zhai Y, Napoli J L. Cloning of a cDNA for liver microsomal retinol dehydrogenase. A tissue specific, short-chain alcohol dehydrogenase. J Biol Chem. 270:1995;3900-3904.
13. Chai X, Zhai Y, Popescu G, Napoli J L. Cloning of a cDNA for second retinol dehydrogenase type II. J Biol Chem. 270:1995;28408-28412.
14. Chai X, Zhai Y, Napoli J L. Cloning of a rat cDNA encoding retinol dehydrogenase isozyme type III. Gene. 169:1996;219-222.
15. Chai X, Zhai Y, Napoli J L. cDNA cloning and characterization of a cis-retinol/3α hydroxysterol short-chain dehydrogenase. J Biol Chem. 272:1997;33125-33131.
16. Su J, Chai X, Kahn B, Napoli J L. cDNA cloning, tissue distribution, and substrate characteristics of a cis-retinol/3α-hydroxysterol short-chain dehydrogenase isozyme. J Biol Chem. 273:1998;17910-17916.
17. Biswas M G, Russell D W. Expression cloning and characterization of oxidative 17β- and 3α-hydroxysteroid dehydrogenases from rat and human prostate. J Biol Chem. 272:1997;16959-15966.
18. Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A, Struhl K. Current Protocols in Molecular Biology. 1991;Wiley, New York.
19. Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
20. Markova N G, Marekov L N, Chipev C C, Gan S-Q, Idler W W, Steinert P M. Profilaggrin is a major epidermal calcium-binding protein. Mol Cell Biol. 13:1993;613-625.
21. Nuovo G J, Forde A. An improved system for reverse transcriptase in situ PCR. J Histotechnol. 18:1995;295-299.
22. Jang S-I, Steinert P M, Markova N G. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J Biol Chem. 271:1996;24105-24114.
23. Rheinwald J G, Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell. 6:1975;331-334.
24. Harrison E H, Blaner W S, Goodman D S, Ross A C. Subcellular localization of retinoids, retinoid-binding proteins, and acyl-CoA:retinol acyltransferase in rat liver. J Lipid Res. 28:1987;973-981.
25. Bradford M M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
26. Randolph R K, Simon M. Characterization of retinol metabolism in cultured human keratinocytes. J Biol Chem. 268:1993;9198-9205.
27. Kozak M. The scanning model for translation: An update. J Cell Biol. 108:1989;229-235.
28. Simon A, Hellman U, Wernstedt C, Eriksson U. The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases. J Biol Chem. 270:1996;1107-1112.
29. Driessen C AGG, Janssen B PM, Winkens H J, van Vugt A HM, de Leeuw T LM, Janssen J JM. Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase. Invest Ophtalmol Visual Sci. 36:1995;1988-1996.
30. Mertz J R, Shang E, Piantedosi R, Wei S, Wolgemuth D J, Blaner W S. Identification and characterization of a stereospecific human enzyme that catalizes 9-cis-retinol oxidation. A possible role in 9-cis-retinoic acid formation. J Biol Chem. 272:1997;11744-117431.
31. Jornvall H, Shafqat J, El-Ahmad M, Hjelmqvist, Persson B, Danielsson O. Alcohol dehydrogenase variability. Evolutionary and functional conclusions from characterization of further variants. Enzymol Mol Biol Carbonyl Metab. 6:1996;403-415.
32. Bucher P, Trifonov E. Compilation and analysis of eucariotic POL II promoter sequences. Nucleic Acids Res. 14:1986;10009-10026.
33. Soares M B, Schon E, Efstratiadis A. Rat LINE1: The origin and evolution of a family of long interspersed middle repetitive DNA elements. J Mol Evol. 22:1985;117-133.
34. Gough W H, VanOoteghem S, Sint T, Kedishvili N Y. cDNA cloning and characterization of new human microsomal NAD+-dependent dehydrogenase that oxidizes all trans-retinol and 3α-hydroxysteroids. J Biol Chem. 273:1998;19778-19785.
35. Kurata S, Itami S, Sonoda T, Takayasu S. Intranuclear androgen concentrations in facial skin. J Dermatol Sci. 2:1991;75-78.
36. Sonoda T, Itami S, Kurata S, Takayasu S. Testosterone metabolism by cultured human beard outer root sheath cells in comparison with epidermal keratinocytes. J Dermatol Sci. 6:1993;214-218.
37. Takayasu S, Wakimoto H, Itami S, Sano S. Activity of testosterone 5α-reductase in various tissues of human skin. J Invest Dermatol. 74:1980;187-191.
38. Milewich L, Kaimal V, Shaw C B, Sontheimer R D. Epidermal keratinocytes: A source of a 5α-dihydrotestosterone production in human skin. J Clin Endocrinol Metab. 62:1986;739-746.
39. Kurata S, Itami S, Komada S, Takayasu S. Intranuclear androgen and cytosolic receptor concentrations in the axillary skin of osmidrosis. Arch Dermatol Res. 282:1990;33-37.
40. Attar P S, Wertz P W, McArthur M, Imakado S, Bickenbach J R, Roop D R. Inhibition of retinoid signaling in transgenic mice alters lipid processing and disrupts epidermal barrier function. Mol Endocrinol. 11:1997;792-800.
41. Tavakkol A, Griffiths C EM, Keane K M, Palmer R D, Voorhees J J. Cellular localization of mRNA for cellular retinoic acid-binding protein II and nuclear retinoic acid receptor-γ 1 in retinoic acid-treated skin. J Invest Dermatol. 99:1992;146-150.
42. Randolph R K, Simon M. Metabolism of all-trans retinoic acid by cultured human epidermal keratinocytes. J Lipid Res. 38:1997;1374-1383.
43. Kurlandsky S B, Duell E A, Kang S, Voorhees J J, Fisher G J. Auto-regulation of retinoic acid biosynthesis through regulation of retinol esterification in human keratinocytes. J Biol Chem. 271:1996;15346-15342.
44. Chatellard-Gruaz D, Randolph R K, Hagens G, Saurat J-H, Siegenthaler G. Differentiation of human epidermal keratinocytes is accompanied by increased expression of CRABP-II and increased cellular concentration of retinoic acids: Retention of newly synthesized retinoic acids by CRABP-II. J Lipid Res. 39:1998;1421-1429.
45. Siegenthaler G, Saurat J-H, Ponec M. Retinol and retinal metabolism. Biochem J. 268:1990;371-378.
46. Haselbeck R J, Ang H L, Duester G. Class IV alcohol/retinol dehydrogenase localization in epidermal basal layer: Potential site of retinoic acid synthesis during skin development. Dev Dynam. 208:1997;447-453.
47. Kurlandsky S B, Xiao J-H, Duell E A, Voorhees J J, Fisher G J. Biological activity of all trans retinoic acid requires metabolic conversion to all-trans retinoic acid and is mediated through activation of nuclear retinoid receptors in human keratinocytes. J Biol Chem. 269:1994;32821-32827.
48. Russell D W, Wilson J D. Steroid 5α-reductase: Two genes/two enzymes. Annu Rev Biochem. 63:1994;25-61.
49. George F W, Russell D W, Wilson J D. Feed-forward control of prostate growth: Dihydrotestosterone induces expression of its own biosynthetic enzyme, steroid 5α-reductase. Proc Natl Acad Sci USA. 88:1991;8044-8047.