Gene dosage by quantitative real-time PCR

BioTechniques

Volumn 27, Issue 2, 1999, Pages 228-232

  Boulay, Jean Louis ^a   Reuter, J ^a   Ritschard, R ^a   Terracciano, L ^b   Herrmann, R ^a   Rochlitz, C ^a  

^a UNIVERSITY HOSPITAL BASEL   (Switzerland)

^b CYTOS BIOTECHNOLOGY AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

TAQ POLYMERASE;

ACCURACY; ARTICLE; COLON CARCINOMA; GENE DELETION; GENE DOSAGE; HETEROZYGOSITY LOSS; HUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; SEX DETERMINATION;

EID: 0032800485     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99272bm03     Document Type: Article

References (12)

1. Eppert, K., S.W. Scherer, H. Ozcelik, R. Pirone, R. Hoodless, H. Kim, L.C. Tsui, B. Bapat et al. 1996. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell 86:543-552.
2. Fearon, E.R., K.R. Cho, J.M. Nigro, S.E. Kern, J.W. Simons, J.M. Ruppert, S.R. Hamilton, A.C. Preisinger et al. 1990. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science 247:49-56.
3. Hahn, S.A., M. Schutte, A.T.M.S. Hoque, C.A. Moskaluk, L.T. da Costa, E. Rozenblum, C.L. Weinstein, A. Fischer et al. 1996. DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science 271:350-353.
4. Heid, C.A., J. Stevens, K.J. Livak and P.M. Williams. 1996. Real time quantitative PCR. Genome Res. 6:986-994.
5. Heim, S. and F. Mitelman. 1987. Cancer Cytogenetics. Liss, New York.
6. Higuchi, R., C. Fockler, G. Dollinger and R. Watson. 1993. Kinetic PCR: real-time monitoring of DNA amplification reactions. Bio/Technology 11:1026-1030.
7. Kenmochi, N., T. Kawaguchi, S. Rozen, E. Davis, N. Goodman, T.J. Hudson, T. Tanaka and D.C. Page. 1998. A map of 75 human ribosomal protein genes. Genome Res. 8:509-523.
8. Laborda, J. 1991. 36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic ribosomal phosphoprotein PO. Nucleic Acids Res. 19:3998.
9. Livak, K.J., S.J.A. Flood, J. Marmaro, W. Giusti and K. Deetz. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 4:357-362.
10. Masiakowski, P., R. Breathnach, J. Bloch, F. Gannon, A. Krust and P. Chambon. 1982. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 10:7895-7903.
11. Vogelstein, B., E.R. Fearon, S.R. Hamilton, S.E. Kern, A.C. Preisinger, M. Leppert, Y. Nakamura, R. White, A.M. Smits and J.L. Bos. 1988. Genetic alterations during colorectal-tumor development. N. Engl. J. Med. 319:525-532.
12. Wang, A.M., M.V. Doyle and D.F. Mark. 1989. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86:9717-9721.