Expression and immunogenicity of a mutant diphtheria toxin molecule, CRM197, and its fragments in Salmonella typhi vaccine strain CVD 908-htrA

Infection and Immunity

Volumn 67, Issue 8, 1999, Pages 4290-4294

  Orr, Nadav ^a   Galen, James E ^a   Levine, Myron M ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTITOXIN; DIPHTHERIA TOXIN; TYPHOID VACCINE;

ANIMAL EXPERIMENT; ARTICLE; GUINEA PIG; IMMUNIZATION; IMMUNOGENICITY; INTRANASAL DRUG ADMINISTRATION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SUBCUTANEOUS DRUG ADMINISTRATION;

EID: 0032791788     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.67.8.4290-4294.1999     Document Type: Article

References (24)

1. Audibert, F., M. Jolivet, L. Chedid, J. E. Alouf, P. Boquet, P. Rivaille, and O. Siffert. 1981. Active antitoxic immunization by a diphtheria toxin synthetic oligopeptide. Nature 289:593-594.
2. Barbieri, J. T., D. Armellini, J. Molkentin, and R. Rappuoli. 1992. Construction of a diphtheria toxin A fragment-C180 peptide fusion protein which elicits a neutralizing antibody response against diphtheria toxin and pertussis toxin. Infect. Immun. 60:5071-5077.
3. Barry, E. M., O. Gomez-Duarte, S. Chatfield, R. Rappuoli, M. Pizza, G. Losonsky, J. Galen, and M. M. Levine. 1996. Expression and immunogenicity of pertussis toxin S1 subunit-tetanus toxin fragment C fusions in Salmonella typhi vaccine strain CVD 908. Infect. Immun. 64:4172-4181.
4. Bazaral, M., P. J. Goscienski, and R. N. Hamburger. 1973. Characteristics of human antibody to diphtheria toxin. Infect. Immun. 7:130-136.
5. Bigio, M., R. Rossi, D. Nucci, G. Antoni, R. Rappuoli, and G. Ratti. 1987. Conformational changes in diphtheria toxoids. Analysis with monoclonal antibodies. FEBS Lett. 218:271-276.
6. Clements, J. D. 1990. Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heat-labile and heat-stable enterotoxins. Infect. Immun. 58:1159-1166.
7. Galen, J. E., O. G. Gomez-Duarte, G. A. Losonsky, J. L. Halpern, C. S. Lauderbaugh, S. Kaintuck, M. K. Reymann, and M. M. Levine. 1997. A murine model of intranasal immunization to assess the immunogenicity of attenuated Salmonella typhi live vector vaccines in stimulating serum antibody responses to expressed foreign antigens. Vaccine 15:700-708.
8. Giannini, G., R. Rappuoli, and G. Ratti. 1984. The amino-acid sequence of two non-toxic mutants of diphtheria toxin: CRM45 and CRM197. Nucleic Acids Res. 12:4063-4069.
9. Gomez-Duarte, O. G., J. Galen, S. N. Chatfield, R. Rappuoli, L. Eidels, and M. M. Levine. 1995. Expression of fragment C of tetanus toxin fused to a carboxyl-terminal fragment of diphtheria toxin in Salmonella typhi CVD 908 vaccine strain. Vaccine 13:1596-1602.
10. Greenfield, L, M. J. Bjorn, G. Horn, D. Fong, G. A. Buck, R. J. Collier, and D. A. Kaplan. 1983. Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta. Proc. Natl. Acad. Sci. USA 80:6853-6857.
11. Gupta, R. K., R. J. Collier, R. Rappuoli, and G. R. Siber. 1997. Differences in the immunogenicity of native and formalinized cross reacting material (CRM197) of diphtheria toxin in mice and guinea pigs and their implications on the development and control of diphtheria vaccine based on CRMs. Vaccine 15:1341-1343.
12. Hess, J., I. Gentschev, D. Miko, M. Welzel, C. Ladel, W. Goebel, and S. H. Kaufmann. 1996. Superior efficacy of secreted over somatic antigen display in recombinant Salmonella vaccine induced protection against listeriosis. Proc. Natl. Acad. Sci. USA 93:1458-1463.
13. Hess, J., I. Gentschev, G. Szalay, C. Ladel, A. Bubert, W. Goebel, and S. H. Kaufmann. 1995. Listeria monocytogenes p60 supports host cell invasion by and in vivo survival of attenuated Salmonella typhimurium. Infect. Immun. 63:2047-2053.
14. Khan, C. M., B. Villarreal-Ramos, R. J. Pierce, G. Riveau, R. Demarco de Hormaeche, H. McNeill, T. Ali, N. Fairweather, S. Chatfield, A. Capron, et al. 1994. Construction, expression, and immunogenicity of the Schistosoma mansoni P28 glutathione S-transferase as a genetic fusion to tetanus toxin fragment C in a live Aro attenuated vaccine strain of Salmonella. Proc. Natl. Acad. Sci. USA 91:11261-11265.
15. Levine, M. M., J. Galen, E. Barry, F. Noriega, S. Chatfield, M. Sztein, G. Dougan, and C. Tacket. 1996. Attenuated Salmonella as live oral vaccines against typhoid fever and as live vectors. J. Biotechnol. 44:193-196.
16. Lobeck, K., P. Drevet, M. Leonetti, C. Fromen-Romano, F. Ducancel, E. Lajeunesse, C. Lemaire, and A. Menez. 1998. Towards a recombinant vaccine against diphtheria toxin. Infect. Immun. 66:418-423.
17. Makrides, S. C. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60:512-538.
18. Oxer, M. D., C. M. Bentley, J. G. Doyle, T. C. Peakman, I. G. Charles, and A. J. Makoff. 1991. High level heterologous expression in E. coli using the anaerobically-activated nirB promoter. Nucleic Acids Res. 19:2889-2892.
19. Perera, V. Y., and M. J. Corbel. 1990. Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141-157 of fragment A. Epidemiol. Infect. 105:457-468.
20. Rolf, J. M., and L. Eidels. 1993. Structure-function analyses of diphtheria toxin by use of monoclonal antibodies. Infect. Immun. 61:994-1003.
21. Rothel, J. S., P. R. Wood, H. F. Seow, and M. W. Lightowlers. 1997. Urea/DTT solubilization of a recombinant Taenia ovis antigen, 45W, expressed as a GST fusion protein results in enhanced protective immune response to the 45W moiety. Vaccine 15:469-472.
22. Tacket, C. O., M. B. Sztein, G. A. Losonsky, S. S. Wasserman, J. P. Nataro, R. Edelman, D. Pickard, G. Dougan, S. N. Chatfield, and M. M. Levine. 1997. Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans. Infect. Immun. 65:452-456.
23. Zucker, D. R., and J. R. Murphy. 1984. Monoclonal antibody analysis of diphtheria toxin. I. Localization of epitopes and neutralization of cytotoxicity. Mol. Immunol. 21:785-793.
24. Zucker, D. R., J. R. Murphy, and A. M. Pappenheimer, Jr. 1984. Monoclonal antibody analysis of diphtheria toxin. II. Inhibition of ADP-ribosyl-transferase activity. Mol. Immunol. 21:795-800.