Urea/DTT solubilization of a recombinant Taenia ovis antigen, 45W, expressed as a GST fusion protein results in enhanced protective immune response to the 45W moiety

Vaccine

Volumn 15, Issue 5, 1997, Pages 469-472

  Rothel, J S ^a,c   Wood, P R ^b   Seow, H F ^b   Lightowlers, M W ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b CSIRO   (Australia)

^c CSL LIMITED   (Australia)

Author keywords

protein; recombinant; solubilization; urea; vaccine

Indexed keywords

DITHIOTHREITOL; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; IMMUNOGLOBULIN G; PARASITE ANTIGEN; RECOMBINANT PROTEIN; UREA; VACCINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY TITER; ARTICLE; CELL INCLUSION; CONTROLLED STUDY; DRUG FORMULATION; IMMUNE RESPONSE; NONHUMAN; PRIORITY JOURNAL; SHEEP; SOLUBILIZATION; SUBCUTANEOUS DRUG ADMINISTRATION; TAPEWORM INFECTION; VACCINATION;

ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; DITHIOTHREITOL; GLUTATHIONE TRANSFERASE; LINEAR MODELS; MALE; RECOMBINANT FUSION PROTEINS; SHEEP; SOLUBILITY; TAENIA; UREA; VACCINES, SYNTHETIC;

EID: 0030945982     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0264-410X(96)00229-0     Document Type: Article

References (8)

1. Fischer, B.E. Renaturation of recombinant proteins produced as inclusion bodies. Biotech. Adv. 1994, 12, 89-101
2. Johnson, K.S., Harrison, G.B.L and Lighlowlers, M.W. et al. Vaccination against ovine cysticercosis using a defined recombinant antigen. Nature 1989, 338, 585-587
3. Smith, D.B. and Johnson, K.S. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 1988, 67, 31-40
4. Dempster, P.P., Robinson, C.M. and Harrison, G.B.L. Parasite vaccine development: large scale recovery of immunogenic Taenia ovis fusion protein GST-45W(B/X) from Escherichia coli inclusion bodies. Parasitol. Res. 1996, 82, 291-296
5. Rothel, J.S., Lightowlers, M.W., Seow, H.-R, Wood, P.R., Heath, D.D. and Harrison, G.B.L. Immune responses associated with protection in sheep vaccinated with a recombinant antigen from Taenia ovis. Parasite Immunol. 1996, 18, 201-208
6. Lighlowlers, M.W., Waterkeyn, J.G., Rothel, J.S., Gauci, C.G. and Harrison, G.B.L. Host-protective fragments and antibody binding epitopes of Taenia ovis 45W recombinant antigen. Parasite Immunol. 1996, 18, 507-513
7. Billman-Jacobe, H., Hodgson, A.L.M., Lightowlers, M., Wood, P.R. and Radford, A.J. Expression of ovine interferon-gamma in Escherichia coli and Corynebacterium glutamicum. Appl. Environ. Microbiol. 1994, 60, 1641-1645
8. Cartwright, G.A.C., Rothel, J.S. and Lightowlers, M.W. Conventional immunoassays underestimate anti-GST antibody titre. J. Immunol. Meth. 1995, 179, 31-35