Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at the oriC region

FEMS Microbiology Letters

Volumn 176, Issue 1, 1999, Pages 163-167

  Mima, Shinji ^a,b   Yamagachi, Yoshihiro ^a   Kondo, Taemi ^a   Tsuchiya, Tomofusa ^a   Mizushima, Tohru ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

DNA replication; DnaA; N terminal region; Site directed mutagenesis

Indexed keywords

AMINO ACID; DEOXYRIBONUCLEOPROTEIN; DOUBLE STRANDED DNA;

ARTICLE; DNA REPLICATION; ESCHERICHIA COLI; OLIGOMERIZATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

BACTERIAL PROTEINS; DNA REPLICATION; DNA-BINDING PROTEINS; MUTAGENESIS, SITE-DIRECTED; ORIGIN RECOGNITION COMPLEX; VIRAL PROTEINS;

EID: 0032790811     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00233-5     Document Type: Article

References (17)

1. Skarstad, K. and Boye, E. (1994) The initiator protein DnaA: evolution, properties and function. Biochem. Biophys. Acta 1217, 111-130.
2. Sekimizu, K., Bramhill, D. and Kornberg, A. (1987) ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the £coli chromosome. Cell 50, 259-265.
3. Mizushima, T., Sasaki, S., Ohishi, H., Kobayashi, M., Katayama, T., Miki, T., Maeda, M. and Sekimizu, K. (1996) Molecular design of inhibitors of in vitro oriC DNA replication based on the potential to block the ATP binding of DnaA protein. J. Biol. Chem. 271, 25178-25183.
4. Roth, A. and Messer, W. (1995) The DNA binding domain of the initiator protein DnaA. EMBO J. 14, 2106-2111.
5. Carr, K.M. and Kaguni, J.M. (1996) The A184V missense mutation of the dnaAS and dnaA46 alleles confers a defect in ATP binding and thermolability in initiation of Escherichia coli DNA replication. Mol. Microbiol. 20, 1307-1318.
6. Mizushima, T., Takaki, T., Kubota, T., Tsuchiya, T., Miki, T., Katayama, T. and Sekimizu, K. (1998) Site-directed mutational analysis for the ATP binding of DnaA protein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP-binding domain of DnaA protein in vitro and in vivo. J. Biol. Chem. 273, 20847-20851.
7. Mizushima, T., Nishida, S., Kurokawa, K., Katayama, T., Miki, T. and Sekimizu, K. (1997) Negative control of DNA replication by hydrolysis of ATP bound to DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli. EMBO J. 16, 3724-3730.
8. Garner, J. and Crooke, E. (1996) Membrane regulation of the chromosomal replication activity of £coli DnaA requires a discrete site on the protein. EMBO J. 15, 3477-3485.
9. Hase, M., Yoshimi, T., Ishikawa, Y., Ohba, A., Guo, L., Mima, S., Makise, M., Yamaguchi, Y., Tsuchiya, T. and Mizushima, T. (I998) Site-directed mutational analysis for the membrane binding of DnaA protein. Identification of amino acids involved in the functional interaction between DnaA protein and acidic phospholipids. J. Biol. Chem. 273, 28651-28656.
10. Marszalek, J. and Kaguni, J.M. (1994) DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. J. Biol. Chem. 269, 4883-4890.
11. Sutton, M.D. and Kaguni, J.M. (1997) The Escherichia coli dnaA gene: four functional domains. J. Mol. Biol. 274, 546-561.
12. Schaper, S. and Messer, W. (1997) Prediction of the structure of the replication initiator protein DnaA. Proteins 28, 1-9.
13. Kubota, T., Katayama, T., Ito, Y., Mizushima, T. and Sekimizu, K. (1997) Conformational transition of DnaA protein by ATP: structural analysis of DnaA protein, the initiator of Escherichia coli chromosome replication. Biochem. Biophys. Res. Commun. 232, 130-135.
14. Fuller, R.S., Kaguni, J.M. and Kornberg, A. (1981) Proc. Natl. Acad. Sci. USA 78, 7370-7374.
15. Mizushima, T., Yokoyama, K., Mima, S., Tsuchiya, T. and Sekimizu, K. (1997) Inhibition of thymidine transport in dnaA mutants of Escherichia coli. J. Biol. Chem. 272, 21195-21200.
16. Katayama, T. (1994) The mutant DnaAcos protein which overinitiates replication of the Escherichia coli chromosome is inert to negative regulation for initiation. J. Biol. Chem. 269, 22075-22079.
17. Sutton, M.D., Carr, K.M., Vicente, M. and Kaguni, J.M. (1998) Escherichia coli DnaA protein. The N-terminal domain and loading of DnaB helicase at the E. coli chromosomal origin. J. Biol. Chem. 273, 34255-34262.