The A184V missense mutation of the dnaA5 and dnaA46 alleles confers a defect in ATP binding and thermolability in initiation of Escherichia coli DNA replication

Molecular Microbiology

Volumn 20, Issue 6, 1996, Pages 1307-1318

  Carr, Kevin M ^a   Kaguni, Jon M ^a  

^a Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; PLASMID DNA;

ALLELE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENETICS; BINDING AFFINITY; DNA BINDING; DNA REPLICATION; ESCHERICHIA COLI; GENE MUTATION; MISSENSE MUTATION; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT;

ADENOSINE TRIPHOSPHATE; ALLELES; BACTERIAL PROTEINS; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MUTAGENESIS; POINT MUTATION; REPLICATION ORIGIN; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029901503     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02649.x     Document Type: Article

References (55)

1. Atlung, T., Clausen, E.S., and Hansen, F.G. (1985) Autoregulation of the dnaA gene of Escherichia colt K12. Mol Gen Genet 200: 442-450.
2. Baker, T.A., and Kornberg, A. (1988) Transcriptional activation of initiation of replication from the E. coli chromosomal origin: an RNA-DNA hybrid near oriC. Cell 55: 113-123.
3. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
4. Bramhill, D., and Kornberg, A. (1988a) Duplex opening by DnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome. Cell 52: 743-755.
5. Bramhill, D., and Kornberg, A. (1988b) A model for initiation at origins of DNA replication. Cell 54: 915-918.
6. Braun, R.E., O'Day, K., and Wright, A. (1985) Autoregulation of the DNA replication gene dnaA in E. coli K-12. Cell 40: 159-169.
7. Brendler, T., Abeles, A., and Austin, S. (1995) A protein that binds to the P1 origin core and the oriC 13mer region in a methylation-specific fashion is the product of the host seqA gene. EMBO J 14: 4083-4089.
8. Bullock, W.O., Fernandez, J.M., and Short, J.M. (1987) XL1-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5: 376-378.
9. Burgers, P.M., Kornberg, A., and Sakakibara, Y. (1981) The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of Escherichia coli. Proc Natl Acad Sci USA 78: 5391-5395.
10. Carl, P.L. (1970) Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet 109: 107-122.
11. Cotton, R.G.H., Rodrigues, N.R., and Campbell, R.D. (1988) Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations. Proc Natl Acad Sci USA 85: 4397-4401.
12. Dreusicke, D., Karplus, P.A., and Schulz, G.E. (1988) Refined structure of porcine cytosolic adenylate kinase at 2.1 Å resolution. J Mol Biol 199: 359-371.
13. von Freiesleben, U., Rasmussen, K.V., and Schaechter, M. (1994) SeqA limits DnaA activity in replication from oriC in Escherichia coli. Mol Microbiol 14: 763-772.
14. + strain at various temperatures. Mol Gen Genet 182: 364-366.
15. cos mutant of Escherichia coli K12. Evidence for DnaA-DnaB interactions. J Mol Biol 179: 171-183.
16. Fuller, R.S., Kaguni, J.M., and Kornberg, A. (1981) Enzymatic replication of the origin of the Escherichia coli chromosome. Proc Natl Acad Sci USA 78: 7370-7374.
17. Fuller, R.S., and Kornberg, A. (1983) Purified DnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication. Proc Natl Acad Sci USA 80: 5817-5821.
18. Fuller, R.S., Funnell, B.E., and Kornberg, A. (1984) The DnaA protein complex with the E coli chromosomal replication origin (oriC) and other DNA sites. Cell 38: 889-900.
19. Funnell, B.E., Baker, T.A., and Kornberg, A. (1987) In vitro assembly of a prepriming complex at the origin of the Escherichia coli chromosome. J Biol Chem 262: 10327-10334.
20. Georgopoulos, C. (1992) The emergence of the chaperone machines. Trends Biochem Sci 17: 295-299.
21. Hansen, F.G., Koefoed, S., and Atlung, T. (1992) Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli. Mol Gen Genet 234: 14-21.
22. Hirota, Y., Ryter, A., and Jacob, F. (1968) Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol 33: 677-693.
23. Hirota, Y., Mordoh, J., and Jacob, F. (1970) On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol 53: 369-387.
24. Hupp, T.R., and Kaguni, J.M. (1993a) Activation of DnaA5 protein by GrpE and DnaK heat shock proteins in initiation of DNA replication in Escherichia coli. J Biol Chem 268: 13137-13142.
25. Hupp, T.R., and Kaguni, J.M. (1993b) Activation of mutant forms of DnaA protein of Escherichia coli by DnaK and GrpE proteins occurs prior to DNA replication. J Biol Chem 268: 13143-13150.
26. Hupp, T.R., and Kaguni, J.M. (1993c) DnaA5 protein is thermolabile in initiation of replication from the chromosomal origin of Escherichia coli. J Biol Chem 268: 13128-13136.
27. Hwang, D.S., and Kaguni, J.M. (1988a) Interaction of DnaA46 protein with a stimulatory protein in replication from the Escherichia coli chromosomal origin. J Biol Chem 263: 10633-10640.
28. Hwang, D.S., and Kaguni, J.M. (1988b) Purification and characterization of the dnaA46 gene product. J Biol Chem 263: 10625-10632.
29. Hwang, D.S., and Kaguni, J.M. (1991) DnaK protein stimulates a mutant form of DnaA protein in Escherichia coli DNA replication. J Biol Chem 266: 7537-7541.
30. Hwang, D.S., Crooke, E., and Kornberg, A. (1990) Aggregated DnaA protein is dissociated and activated for DNA replication by phospholipase or DnaK protein. J Biol Chem 265: 19244-19248.
31. Jurnak, F. (1985) Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins. Science 230: 32-36.
32. Kaguni, J.M., Fuller, R.S., and Kornberg, A. (1982) Enzymatic replication of E. coli chromosomal origin is bidirectional. Nature 296: 623-627.
33. cos, of Escherichia coli. J Biol Chem 269: 12698-12703.
34. Kucherer, C., Lother, H., Kolling, R., Schauzu, M.A., and Messer, W. (1986) Regulation of transcription of the chromosomal dnaA gene of Escherichia coli. Mol Gen Genet 205: 115-121.
35. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
36. Lu, M., Campbell, J.L., Boye, E., and Kleckner, N. (1994) SeqA: a negative modulator of replication initiation in E. coli. Cell 77: 413-426.
37. Maaloe, O., and Kjeldgaard, N.O. (1966) Control of Macromolecular Synthesis. New York: W.A. Benjamin.
38. Marszalek, J., and Kaguni, J.M. (1994) DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. J Biol Chem 269: 4883-4890.
39. Marszalek, J., Zhang, W., Hupp, T.R., Carr, K.M., Maugulies, C., and Kaguni, J.M. (1996) Domains of DnaA protein involved in interaction with DnaB protein, and in unwinding the E. coli chromosomal origin. J Biol Chem, in press.
40. Messer, W., and Weigel, C. (1996) Initiation of chromosome replication. In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology, 2nd edn. Neidhardt, F.C., Ingraham, J.L., Low, K.B., Magasanik, B., Schaechter, M., and Umbarger, H.E. (eds). Washington, DC: American Society for Microbiology, in press.
41. Muller, C.W., and Schulz, G.E. (1992) Structure of the complex between adenylate kinase from Escherichia coli and the inhibitor Ap5A refined at 1.9 Å resolution. A model for a catalytic transition state. J Mol Biol 224: 159-177.
42. Pai, E.F., Krengel, U., Petsko, G.A., Goody, R.S., Kabsch, W., and Wittinghofer, A. (1990) Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 Å resolution: implications for the mechanism of GTP hydrolysis. EMBO J 9: 2351-2359.
43. Roth, A., and Messer, W. (1995) The DNA binding domain of the initiator protein DnaA. EMBO J 14: 2106-2111.
44. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
45. Saraste, M., Sibbald, P.R., and Wittinghofer, A. (1990) The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biochem Sci 15: 430-434.
46. Sekimizu, K., Bramhill, D., and Kornberg, A. (1987) ATP activates DnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome. Cell 50: 259-265.
47. Sekimizu, K., Yung, B.Y., and Kornberg, A. (1988) The DnaA protein of Escherichia coli: abundance, improved purification, and membrane binding. J Biol Chem 263: 7136-7140.
48. Skarstad, K., von Meyenburg, K., Hansen, F.G., and Boye, E. (1988) Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles. J Bacteriol 170: 852-858.
49. Skarstad, K., Lobner-Olesen, A., Atlung, T., von Meyenburg, K., and Boye, E. (1989) Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein. Mol Gen Genet 218: 50-56.
50. Slater, S., Wold, S., Lu, M., Boye, E., Skarstad, K., and Kleckner, N. (1995) E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration. Cell 82: 927-936.
51. Story, R.M., and Steitz, T.A. (1992) Structure of the RecA protein-ADP complex. Nature 355: 374-376.
52. Wang, Q.P., and Kaguni, J.M. (1987) Transcriptional repression of the dnaA gene of Escherichia coli by DnaA protein. Mol Gen Genet 209: 518-525.
53. Yung, B.Y., and Kornberg, A. (1989) The DnaA initiator protein binds separate domains in the replication origin of Escherichia coli. J Biol Chem 264: 6146-6150.
54. Yung, B.Y., Crooke, E., and Kornberg, A. (1990) Fate of the DnaA initiator protein in replication at the origin of the Escherichia coli chromosome in vitro. J Biol Chem 265: 1282-1285.
55. Zylicz, M., and Georgopoulos, C. (1984) Purification and properties of the Escherichia coli DnaK replication protein. J Biol Chem 259: 8820-8825.