Detection and assay of proteases using calf lens β-crystallin aggregate as substrate

Journal of Biochemical and Biophysical Methods

Volumn 40, Issue 1-2, 1999, Pages 49-55

  Trivedi, Vishwa D ^a   Raman, Bakthisaran ^a   Ramakrishna, Tangirala ^a   Rao, Ch Mohan ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Protease detection; Turbidity clearance

Indexed keywords

BETA CRYSTALLIN; PROTEINASE; TRYPSIN;

ANIMAL TISSUE; ARTICLE; CATTLE; ENZYME ASSAY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FOLDING; TURBIDITY;

ANIMALS; CATTLE; CRYSTALLINS; ENDOPEPTIDASES; HYDROGEN-ION CONCENTRATION; KINETICS; NEPHELOMETRY AND TURBIDIMETRY; PROTEASE INHIBITORS; PROTEIN DENATURATION; PROTEIN FOLDING; SOLUBILITY; TRYPSIN;

EID: 0032790118     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(99)00021-4     Document Type: Article

References (11)

1. Sarath G., de la Motte R.S., Wagner F.W. Protease assay methods. Beynon R.G., Bond J.S. Proteolytic enzymes: a practical approach. 1989;25-55 IRL Press, Oxford.
2. Hsia C.Y., Ganshaw G., Paech C., Murray C.J. Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors. Anal Biochem. 242:1996;221-227.
3. Voss E.W., Workman C.J., Mummert M.E. Detection of protease activity using a fluorescence-enhancement globular substrate. Biotechniques. 20:1996;286-291.
4. Hagen H-E., Klager S.L., McKerrow J.H., Ham P.J. A simple assay for the detection of native and recombinant protease activity. Anal Biochem. 251:1997;121-122.
5. Yoshioka H., Oyamada I., Usuku G. An assay of collagenase activity using enzyme-linked immunosorbent assay for mammalian collagenases. Anal Biochem. 166:1987;172-177.
6. Nestler H.P., Doseff A. A two-dimensional diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique to screen for protease substrates in protein mixtures. Anal Biochem. 251:1997;122-125.
7. Koritsas V.M., Atkinson H.J. An assay for detecting nanogram levels of proteolytic enzymes. Anal Biochem. 227:1995;22-26.
8. Raman B., Ramakrishna T., Rao C.M. Rapid refolding studies on the chaperone-like α-crystallin: effect of α-crystallin on refolding of β- and γ-crystallin. J Biol Chem. 270:1995;19888-19892.
9. Bloemendal H. The lens proteins. Bloemendal H. Molecular and cellular biology of the eye lens. 1981;1-47 Wiley, New York.
10. Sipos T., Merkel J.R. An effect of calcium on the activity, heat stability, and structure of trypsin. Biochemistry. 9:1970;2766-2775.
11. Kassell B. Protease inhibitors from egg white. Perlmann G.E., Lorand L. Methods in enzymology. 19:1970;890-906 Academic Press, New York.