Detection of protease activity using a fluorescence-enhancement globular substrate

BioTechniques

Volumn 20, Issue 2, 1996, Pages 286-291

  Voss Jr , Edward W ^a   Workman, Creg J ^a   Mummert, Mark E ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; CHYMOTRYPSIN A; FLUORESCEIN ISOTHIOCYANATE; GLOBULAR PROTEIN; LYSINE; PRONASE; PROTEINASE; PROTEINASE K; REDUCING AGENT; THIOCARBAMIC ACID DERIVATIVE; THIOL REAGENT; TRYPSIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE; NONHUMAN; PROTEIN DEGRADATION;

BOVINAE;

EID: 0030060185     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96202rr06     Document Type: Article

References (8)

1. Bedzyk, W.D., C.A. Swindlehurst and E.W. Voss, Jr. 1992. Relative binding properties of fluorescein and 9-hydroxyphenylfluoron (HPF) with murine monoclonal anti-fluorescein antibodies. Biochem Biophys Acta 1119:27-34.
2. Bolger, R. and W. Checovich. 1994. A new protease activity assay using fluorescence polarization. BioTechniques 17:585-589.
3. Förster, T. 1948. Intermolecular energy migration and fluorescence. Ann. Phys. (Leipzig) 2:55-75.
4. Herron, J.N. and E.W. Voss, Jr. 1983. Analysis of heterogeneous dissociation kinetics in polyclonal populations of rabbit anti-fluorescyl-lgG antibodies Mol Immunol. 20:1323-1332.
5. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
6. Twining, S.S. 1984. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal Biochem. 140:30-34.
7. Watt, R.M. and E.W. Voss, Jr. 1978. Characterization of affinity-labeled fluorescyl ligand to specifically-purified rabbit IgG antibodies. Immunochemistry 15:875-882.
8. Weber, G. 1952. Polarization of the fluorescence of macromolecules I. Theory and experimental method. Biochem. J. 51:145-155.