Random mutagenesis of the pomA gene encoding a putative channel component of the Na+-driven polar flagellar motor of Vibrio alginolyticus

Microbiology

Volumn 145, Issue 7, 1999, Pages 1759-1767

  Kojima, Seiji ^a   Kuroda, Mitsuyo ^a   Kawagishi, Ikuro ^a   Homma, Michio ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Flagella; Motor; pomA; Sodium ion; Vibrio

Indexed keywords

BACTERIAL PROTEIN; HYDROXYLAMINE; MEMBRANE PROTEIN; SODIUM CHANNEL; SODIUM ION;

ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; FLAGELLUM; GENE MAPPING; IMMUNOBLOTTING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; VIBRIO ALGINOLYTICUS;

VIBRIO ALGINOLYTICUS;

EID: 0032780519     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-7-1759     Document Type: Article

References (41)

1. Asai, Y., Kojima, S., Kato, H., Nishioka, N., Kawagishi, I. & Homma, M. (1997). Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. J Bacteriol 179, 5104-5110.
2. +-driven flagellar motors of alkalophilic Bacillus strains by the amiloride analog phenamil. J Bacteriol 172, 1634-1639.
3. Bartolomé, B., Jubete, Y., Martinez, E. & Cruz, F. D. (1991). Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene 102, 75-78.
4. Blair, D. F. (1995). How bacteria sense and swim. Annu Rev Microbiol 49, 489-522.
5. Blair, D. F. & Berg, H. C. (1988). Restoration of torque in defective flagellar motors. Science 242, 1678-1681.
6. Blair, D. F. & Berg, H. C. (1990). The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell 60, 439-449.
7. Blair, D. F. & Berg, H. C. (1991). Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction. J Mol Biol 221, 1433-1442.
8. Blair, D. F., Kim, D. Y. & Berg, H. C. (1991). Mutant MotB proteins in Escherichia coli. J Bacteriol 173, 4049-4055.
9. Block, S. M. & Berg, H. C. (1984). Successive incorporation of force-generating units in the bacterial rotary motor. Nature 309, 470-472.
10. Chun, S. Y. & Parkinson, J. S. (1988). Bacterial motility: membrane topology of the Escherichia coli MotB protein. Science 239, 276-278.
11. De Mot, R. & Vanderleyden, J. (1994). The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol Microbiol 12, 333-334.
12. Dean, G. D., Macnab, R. M., Stader, J., Matsumura, P. & Burks, C. (1984). Gene sequence and predicted amino acid sequence of the MotA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. J Bacteriol 159, 991-999.
13. Francis, N. R., Sosinsky, G. E., Thomas, D. & DeRosier, D. J. (1994). Isolation, characterization and structure of bacterial flagellar motors containing the switch complex. J Mol Biol 235, 1261-1270.
14. Garza, A. G., Harris, H. L., Stoebner, R. A. & Manson, M. D. (1995). Motility protein interactions in the bacterial flagellar motor. Proc Natl Acad Sci USA 92, 1970-1974.
15. Garza, A. G., Biran, R., Wohlschlegel, J. A. & Manson, M. D. (1996). Mutations in motB suppressible by changes in stator or rotor components of the bacterial flagellar motor. J Mol Biol 258, 270-285.
16. Grant S. G., Jessee, J., Bloom, F. R. & Hanahan, D. (1990). Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli. Proc Natl Acad Sci USA 87, 4645-4649.
17. +-driven bacterial flagellar motors. J Bioenerg Biomembr 21, 705-716.
18. Irikura, V. M., Kihara, M., Yamaguchi, S., Socken, H. & Macnab, R. M. (1993). Salmonella typhimurium fliG and fliN mutations causing defects in assembly, rotation, and switching of the flagellar motor. J Bacteriol 175, 802-810.
19. 0 ATPase in Escherichia coli by homologous recombination. Eur J Biochem 218, 937-944.
20. 1-ATPase. Eur J Biochem 222, 615-623.
21. Kawagishi, I., Okunishi, I., Homma, M. & Imae, Y. (1994). Removal of the periplasmic DNase before electroporation enhances efficiency of transformation in a marine bacterium Vibrio alginolyticus. Microbiology 140, 2355-2361.
22. +-driven flagellar motor resistant to phenamil, an amiloride analog, caused by mutations in putative channel component. J Mol Biol 285, 1537-1547.
23. Lloyd, S.A. & Blair, D. F. (1997). Charged residues of the rotor protein FliG essential for torque generation in the flagellar motor of Escherichia coli. J Mol Biol 266, 733-744.
24. Lloyd, S. A., Tang, H., Wang, X., Billings, S. & Blair, D. F. (1996). Torque generation in the flagellar motor of Escherichia coli: evidence of a direct role for FliG but not for FliM or FliN. J Bacteriol 178, 223-231.
25. McCarter, L. L. (1994a). MotX, the channel component of the sodium-type flagellar motor. J Bacteriol 176, 5988-5998.
26. McCarter, L. L. (1994b). MotY, a component of the sodium-type flagellar motor. J Bacteriol 176, 4219-4225.
27. Nishioka, N., Furuno, M., Kawagishi, I. & Homma, M. (1998). Flagellin-containing membrane vesicles excreted from Vibrio alginolyticus mutants lacking a polar-flagellar filament. J Biochem 123, 1169-1173.
28. Okunishi, I., Kawagishi, I. & Homma, M. (1996). Cloning and characterization of motY, a gene coding for a component of the sodium-driven flagellar motor in Vibrio alginolyticus. J Bacteriol 178, 2409-2415.
29. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
30. Shah, D. S. & Sockett, R. E. (1995). Analysis of the motA flagellar motor gene from Rhodobacter sphaeroides, a bacterium with a unidirectional, stop-start flagellum. Mol Microbiol 17, 961-969.
31. Sharp, L. L., Zhou, J. & Blair, D. F. (1995a). Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation in Escherichia coli. Biochemistry 34, 9166-9171.
32. Sharp, L. L., Zhou, J. & Blair, D. F. (1995b). Features of MotA proton channel structure revealed by tryptophan-scanning mutagenesis. Proc Natl Acad Sci USA 92, 7946-7950.
33. Sockett, H., Yamaguchi, S., Kihara, M., Irikura, V. & Macnab, R. M. (1992). Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium. J Bacteriol 174, 793-806.
34. Stader, J., Matsumura, P., Vacante, D., Dean, G. E. & Macnab, R. M. (1986). Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. J Bacteriol 166, 244-252.
35. Stolz, B. & Berg, H. C. (1991). Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli. J Bacteriol 173, 7033-7037.
36. +-driven flagellar motors of alkalophilic Bacillus. J Biol Chem 263, 8215-8219.
37. Yamaguchi, S., Fujita, H., Ishihara, H., Aizawa, S. & Macnab, R. M. (1986). Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J Bacteriol 166, 187-193.
38. Zhou, J. & Blair, D. F. (1997). Residues of the cytoplasmic domain of MotA essential for torque generation in the bacterial flagellar motor. J Mol Biol 273, 428-439.
39. Zhou, J., Fazzio, R. T. & Blair, D. F. (1995). Membrane topology of the MotA protein of Escherichia coli. J Mol Biol 251, 237-242.
40. Zhou, J., Sharp, L. L., Tang, H. L., Lloyd, S. A., Billings, S., Braun, T. F. & Blair, D. F. (1998a). Function of protonatable residues in the flagellar motor of Escherichia coli: a critical role for Asp32 of MotB. J Bacteriol 180, 2729-2735.
41. Zhou, J., Lloyd, S. A. & Blair, D. F. (1998b). Electrostatic interactions between rotor and stator in the bacterial flagellar motor. Proc Natl Acad Sci USA 95, 6436-6441.