Crystallization and preliminary X-ray analysis of active site-inhibited human coagulation factor VIIa (des-Gla)

Journal of Structural Biology

Volumn 125, Issue 1, 1999, Pages 90-93

  Johnson, Daniel J D ^a   Nugent, Philip G ^a   Tuddenham, Edward G D ^a   Harlos, Karl ^b   Kemball Cook, Geoffrey ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Coagulation; Crystallization; Factor VII; Haemostasis; Protein purification; Recombinant expression; X ray diffraction

Indexed keywords

BLOOD CLOTTING FACTOR 7A;

ARTICLE; BLOOD CLOTTING; CHEMICAL STRUCTURE; CRYSTAL STRUCTURE; CRYSTALLIZATION; HEMOSTASIS; HUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; X RAY DIFFRACTION;

EID: 0032778476     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1998.4078     Document Type: Article

References (6)

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2. Kemball-Cook G., Garner I., Imanaka Y., Nishimura T., O'Brien D. P., Tuddenham E. G. D., McVey J. H. High level production of human blood coagulation factors VII and XI using a new mammalian expression vector. Gene. 139:1994;275-279.
3. 100. J. Biol. Chem. 273:1998;8516-8521.
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5. Padmanabhan K., Padmanabhan K. P., Tulinsky A. Structure of human des(1-45) factor Xa at 2.2 Å resolution. J. Mol. Biol. 232:1993;947-966.
6. Teng T.-Y. Mounting of crystals for macromolecular crystallography in a free-standing thin film. J. Appl. Crystallogr. 23:1990;387-391.