Determination of the genome size of Ehrlichia spp., using pulsed field gel electrophoresis

FEMS Microbiology Letters

Volumn 176, Issue 1, 1999, Pages 73-78

  Rydkina, Elena ^a   Roux, Véronique ^a   Raoult, Didier ^a,b  

^a AIX MARSEILLE UNIVERSITY   (France)

^b INSTITUTE OF BIOMEDICAL CHEMISTRY   (Russian Federation)

Author keywords

Ehrlichia; Genome size; Pulsed field gel electrophoresis

Indexed keywords

ARTICLE; BACTERIAL GENETICS; EHRLICHIA; EHRLICHIA CHAFFEENSIS; GENOME; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS;

ANIMALS; EHRLICHIA; ELECTROPHORESIS, GEL, PULSED-FIELD; GENOME, BACTERIAL; HORSES; HUMANS; PHYLOGENY; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

EID: 0032764488     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00221-9     Document Type: Article

References (17)

1. Rikihisa, Y. (1991) The tribe Ehrlicliiae and ehrlichial diseases. Clin. Microbiol. Rev. 4, 286-308.
2. Walker, D.H. and Dumler, J.S. (1996) Emergence of the ehrlichioses as human health problems. Emerging Infect. Dis. 2, 18-29.
3. Chen, S.-M., Dumler, J.S., Bakken, J.S. and Walker, D. (1994) Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32, 589-595.
4. Dawson, J.E., Anderson, B.E., Fishbein, D.B., Sanchez, J.L., Goldsmith, C.S., Wilson, K.H. and Duntley, C.W. (1991) Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J. Clin. Microbiol. 29, 2741-2745.
5. Dumler, J.S., Chen, S.-M., Asanovich, K., Trigiani, E., Popov, V.L. and Walker, D.H. (1995) Isolation and characterization of a new strain of Ehrlichia cliaffecnsis from a patient with nearly fatal monocytic ehrlichiosis. J. Clin. Microbiol. 33, 1704-1711.
6. Sumner, J.W., Nicholson, W.L. and Massung, R.F. (1997) PCR amplification and comparison of nucleotide sequences from the groESL heat-shock opcron of Elirlicliin species J. Clin. Microbiol. 35, 2087-2092.
7. Wen, B., Rikihisa, Y., Yamamoto, S., Kawabata, N. and Fuerst, P.A. (1996) Characterization of the SF agent, an £/,r. licliia sp. isolated from the fluke Slellantchasnna falcntus, by 16S rRNA base sequence, serological, and morphological analysis. Int. J. Syst. Bacteriol. 46, 149-154.
8. Chen, S.-M., Dumler, J.S., Feng, H.-M. and Walker, D.H. (1994) Identification of the antigenic constituents of Elirlichia clmffeensis. Am. J. Trop. Med. Hyg. 50, 52-58.
9. Dumler, J.S., Asanovich, K.M., Bakken, J.S., Richter, p., Kimsley, R. and Madigan, J.E. (1995) Serologie cross-reactions among Elirlichia cifiii, Elirlichia phagocytophila, and human granulocytic ehrlichia. J. Clin. Microbiol. 33, 10981103.
10. Schwanz, D.C. and Cantor, C.R. (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37, 67-75.
11. Roux, V. and Raoult, D. (1993) Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel clectrophoresis. J. Bacteriol. 175, 4895-4904.
12. Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Lane, D.J. (1991) 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697-703.
13. Eremeeva, M.E., Roux, V. and Raoult, D. (1993) Determination of genome size and restriction pattern polymorphism of Rickeitsia prowazekii and Rickcilsia typhl by pulsed field electrophoresis. FEMS Microbiol. Lett. 112, 105-112.
14. Alleman, A.R., Kamper, S.M., Viseshakul, N. and Barbel, A.F. (1993) Analysis of the Anaplasnia marginale genome by pulsed-field electrophoresis. J. Gen. Microbiol. 139, 24392444.
15. Frutos, R., Pages, M., Bellis, M., Roizes, G. and Bergoin, M. (19S9) Pulsed-field gel electrophoresis determination of the genome size of obligate inlracellular bacteria belonging to the genera Chlamydia, Rickcttsiella, and Porochlamydia. J. Bacteriol. 171,4511-4513.
16. Weisburg, W. (1989) Polyphyletic origin of bacterial parasites. In: Intracellular Parasitism (Moulder, J.W., Ed.), Ch. 1, pp. 1-15. CRC Press, Boca Raton, FL.
17. de Villiers, E.P., Brayton, K.A., Zweygarth, E. and Allsopp, B.A. (1998) Purification of Comtria ruminantium organisms for use in genome analysis by pulsed-field gel electrophoresis. Ann. N. Y. Acad. Sei. 29, 313-320.