Purification of Cowdria vuminantium organisms for use in genome analysis by pulsed-field gel electrophoresis

Annals of the New York Academy of Sciences

Volumn 849, Issue , 1998, Pages 313-320

  De Villiers, E P ^a   Brayton, K A ^a   Zweygarth, E ^a   Allsopp, B A ^a  

^a ONDERSTEPOORT VETERINARY INSTITUTE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; EXTRACHROMOSOMAL DNA;

ANIMAL CELL; BACTERIAL GENETICS; BACTERIUM ISOLATION; CATTLE; CONFERENCE PAPER; DNA PURIFICATION; EHRLICHIA; GENOME; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS;

ACARI; ANIMALIA; BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; EHRLICHIA; EHRLICHIA RUMINANTIUM;

EID: 0031818749     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1998.tb11064.x     Document Type: Article

References (20)

1. ALLSOPP, B.A., M.T.P. ALLSOPP, J.H. DU PLESSIS & E.S. VISSER. 1996. Uncharacterised Ehrlichia spp. may contribute to clinical heartwater. Annals N.Y. Acad. Sci. 791: 17-23.
2. PROVOST, A. & J.D. BEZUIDENHOUT. 1987. The historical background and global importance of heartwater. Onderstepoort J. Vet. Res. 54: 165-169.
3. OBEREM, P.T. & J.D. BEZUIDENHOUT. 1987. The production of heartwater vaccine. Onderstepoort J. Vet. Res. 54: 485-488.
4. VAN VLIET, A. H. M., F. JONGEJAN, M. VAN KLEEF & B. A. M. VAN DER ZEIJST. 1994. Molecular cloning, sequence analysis, and expression of the gene encoding the immunodominant 32-kilodalton protein of Cowdria ruminantium. Infect. Immun. 62: 1451-1456.
5. MAHAN, S. M., T. C. MCGUIRE, S. M. SEMU, M. V. BOWIE, F. JONGEJAN, F. R. RURANGIRWA & A. F. BARBET. 1994. Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium. Microbiology 140: 2135-2142.
6. WAGHELA, S. D., F. R. RURANGIRWA, S. M. MAHAN, C. E. YUNKER, T. B. CRAWFORD, A. F. BARBET, M. J. BURRIDGE & T. C. MCGUIRE. 1991. A cloned DNA probe identifies Cowdria ruminantiuin in Amblyomma variegatum ticks. J. Clin. Microbiol. 29: 2571-2577.
7. LALLY, N.C., S. NICOLL, E.A. PAXTON, C.M. CARY & K.J. SUMPTION. 1995. The Cowdria ruminantium groE operon. Microbiology 141:2091-100.
8. ALLEMAN, A.R., S.M. KAMPER, N. VISESHAKUL & A.F. BARBET. 1993. Analysis of the Anaplasma marginale genome by pulsed-field electrophoresis. J. Gen. Microbiol. 139: 2439-2444.
9. ROUX, V., M. DRANCOURT & D. RAOULT. 1992. Determination of genome sizes of the Rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis. J. Bacteriol. 174: 7455-7457.
10. DU PLESSIS, J. L. 1985. A method for determining the Cowdria ruminantium infection rate of Amblyomma hebraeum: Effects in mice injected with tick homogenates. Onderstepoort J. Vet. Res. 52: 55-61.
11. BEZUIDENHOUT, J. D., C. L. PATTERSON & B. J. H. BARNARD. 1985. In vitro cultivation of Cowdria ruminantium. Onderstepoort J. Vet. Res. 52: 113-120.
12. VAN KLEEF, M., A.W.H. NEITZ & D.T. DE WAAL. 1993. Isolation and characterization of antigenic proteins of Cowdria ruminantium. Rev. Elev. Med. Vet. Pays. Trop. 46: 157-164.
13. BRAYTON, K.A., J. FEHRSEN, E.P. DE VILLIERS, M. VAN KLEEF & B.A. ALLSOPP. 1997. Construction and initial analysis of a representative λZAPII expression library of the intracellular rickettsia Cowdria ruminantium: Cloning of map1 and three other Cowdria genes. Vet. Parasitol. 72: 185-199.
14. MAHAN, S.M., H.R. ANDREW, N. TEBELE, M.J. BURRIDGE & A.F. BARBET. 1995. Immunisation of sheep against heartwater with inactivated Cowdria ruminantium. Res. Vet. Sci. 58: 46-49.
15. MILTENYI, S., W. MÜLLER, W. WEICHEL & A. RADBRUCH. 1990. High gradient magnetic cell separation with MACS. Cytometry. 11: 231-238.
16. MATHEW, M.K., C.L. SMITH & C.R. CANTOR. 1988. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation process. Biochemistry 27: 9210-9216.
17. HIGHTOWER, R.C., J.B. BLISKA, N.R. COZZARELLI & D.V. SANTI. 1989. Analysis of amplified DNAs from drug-resistant Leismania by orthogonal-field-alteration gel electrophoresis. J. Biol. Chem. 264: 2979-2984.
18. LEVENE, S.D. & B.H. ZIMM. 1987. Separations of open circular DNA using pulsed-field electrophoresis. Proc. Natl. Acad. Sci. USA 84: 4054-4075.
19. BARTON, B.M., G.P. HARDING & A.J. ZUCCARELLI. 1995. A general method for detecting and sizing large plasmids. Anal. Biochem. 226: 235-240.
20. MÉNDEZ-ÁLVAREZ, S., V. PAVÓN, I. ESTEVE, R. GUERRERO & N. GAJU. 1995. Analysis of bacterial genomes by pulsed field gel electrophoresis. Microbiologia Sem 11: 323-336.