Burkholderia cepacia is resistant to the antimicrobial activity of airway epithelial cells

Immunopharmacology

Volumn 44, Issue 3, 1999, Pages 267-272

  Baird, Rosamund M ^a   Brown, Helen ^a   Smith, Anthony W ^a   Watson, Malcolm L ^a  

^a UNIVERSITY OF BATH   (United Kingdom)

Author keywords

defensins; Airway epithelial cells; Burkholderia cepacia; Pseudomonas aeruginosa

Indexed keywords

DEFENSIN; MESSENGER RNA;

ARTICLE; BURKHOLDERIA CEPACIA; CELL LINE; COCULTURE; CONTROLLED STUDY; EPITHELIUM CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INFECTION RESISTANCE; MINIMUM INHIBITORY CONCENTRATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; RESPIRATORY EPITHELIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BETA-DEFENSINS; BURKHOLDERIA CEPACIA; CELL LINE; CELLS, CULTURED; CULTURE MEDIA; DEFENSINS; EPITHELIAL CELLS; GROWTH INHIBITORS; HUMANS; PROTEINS; PSEUDOMONAS AERUGINOSA; RNA, MESSENGER;

EID: 0032761178     PISSN: 01623109     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0162-3109(99)00122-8     Document Type: Article

References (16)

1. Bals R., Wang X., Wu Z., Freeman T., Bafna V., Zasloff M., Wilson J.M. Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J. Clin. Invest. 102:1998;874-880.
2. Bals R., Weiner D.J., Wilson J.M. The innate immune system in cystic fibrosis lung disease. J. Clin. Invest. 103:1999;303-307.
3. Diamond G., Zasloff M., Eck H., Brasseur M., Maloy M.L., Bevins C.L. Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa; peptide isolation and cDNA cloning. Proc. Natl. Acad. Sci. U.S.A. 88:1991;3952-3956.
4. Miller, J.H. Experiments in Molecular Genetics. Cold Spring Harbour, Cold Springer Harbour Press, 1972.
5. Goldman M.J., Anderson G.M., Stolzenberg E.D., Kari U.P., Zasloff M., Wilson J.M. Human β-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis. Cell. 88:1997;553-560.
6. Govan J.R.W., Nelson J.W. Microbiology of lung infection in cystic fibrosis. Br. Med. Bull. 48:1992;912-930.
7. Isles A., Maclusky I., Corey M., Gold R., Prober C., Fleming P., Levison H. Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J. Pediatr. 104:1984;206-210.
8. McCray P.B. Jr., Bentley L. Human airway epithelia express a β-defensin. Am. J. Respir. Cell Mol. Biol. 16:1997;343-349.
9. Meyer J.M., Abdallah M.A. The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physico-chemical properties. J. Gen. Microbiol. 107:1978;319-328.
10. Schnapp D., Harris A. Antibacterial peptides in bronchoalveolar lavage fluid. Am. J. Respir. Cell Mol. Biol. 19:1998;352-356.
11. Schonwetter B.S., Stolzenberg E.D., Zasloff M. Epithelial antibiotics induced at sites of inflammation. Science. 267:1995;1645-1648.
12. Singh, P.K., Jia, H.P., Wiles, K., Liu, L., Conway, B.D., Valore, E.V., Welsh, M.J., Ganz, T., Tack, B.F., McCray Jr., PB, 1998. Constitutive and inducible expression of β-defensin antimicrobial peptides by human airway epithelia. Pediatr. Pulmonol., S15.
13. Smith J.J., Travis S.M., Greenberg E.P., Welsh M.J. Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid. Cell. 85:1996;229-236.
14. Speert D.P., Bond M., Woodman R.C., Curnutte J.T. Infection with Pseudomonas cepacia in chronic granulomatous disease: role of nonoxidative killing by neutrophils in host defense. J. Infect. Dis. 170:1994;1524-1531.
15. Verghese, M.W., Diamond, G., Becker, M.N., Randell, S.H., 1998. Upregulation of human β-defensin 2 by LPS and TNF-α in cultured human bronchial epithelial cells. Role of epithelial cell-expressed CD14. Pediatr. Pulmonol., S15.
16. Wine J.J. The genesis of cystic fibrosis lung disease. J. Clin. Invest. 103:1999;309-312.