RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: Oligomerization property and stoichiometry of the complex with yeast tRNA(Phe)

Biochimie

Volumn 81, Issue 7, 1999, Pages 751-756

  Arluison, Véronique ^a   Batelier, Gérard ^a   Riès Kautt, Madeleine ^a   Grosjean, Henri ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

Author keywords

Aggregation; Dodecyl D maltoside; Oligomerization; Pseudouridine synthetase; tRNA

Indexed keywords

DETERGENT; PHENYLALANINE TRANSFER RNA; PSEUDOURIDINE; SYNTHETASE;

ARTICLE; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; NONHUMAN; OLIGOMERIZATION; PRECIPITATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN RNA BINDING; RNA SYNTHESIS; SACCHAROMYCES CEREVISIAE; STOICHIOMETRY;

SACCHAROMYCES CEREVISIAE;

EID: 0032752966     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(99)80133-3     Document Type: Article

References (17)

1. Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C. Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J. 15:1996;2270-2284.
2. Szweykowska-Kulinska Z., Senger B., Keith G., Fasiolo F., Grosjean H. Intron-dependent formation of pseudouridines in the anticodon of Saccharomyces cerevisiae minor tRNA(Ile). EMBO J. 13:1994;4636-4644.
3. Motorin Y., Keith G., Simon C., Foiret D., Simos G., Hurt E., Grosjean H. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. RNA. 4:1998;856-869.
4. Massenet S., Motorin Y., Lafontaine D.L.J., Hurt E., Grosjean H., Branlant C. Pseudouridine mapping in the S. cerevisiae spliceosomal UsnRNAs reveals that pseudouridine synthase Pus1p exhibit a dual substrate specificity for U2 snRNA and tRNA. Mol. Cell Biol. 19:1999;2142-2154.
5. Roth M., Arnoux B., Ducruix A., Reiss-Husson F. Structure of the detergent phase and protein-detergent interactions on crystals of the wild type (strain Y) Rhodobacter spheroides photochemical reaction center. Biochemistry. 30:1991;9403-9413.
6. Deisenhofer J., Michel H. The photosynthetic reaction centre from the purple vacterium Rhodopseudomonas viridis, EMBO J. 8:1989;2149-2169.
7. Kuczera K., Gao J., Tidor B., Karplus M. Free energy of slicking: a simulation analysis. Proc. Natl. Acad. Sci. USA. 87:1990;8481-8485.
8. Zardaneta G., Horowitz P.M. Detergent, liposome, and micelle-assisted protein refolding. Anal. Biochem. 223:1994;1-6.
9. Arluison V., Hountondji C., Robert B., Grosjean H. Transfer RNA-pseudouridine synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition. Biochemistry. 37:1998;7268-7276.
10. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
11. Philo J.S. Measuring sedimentation, diffusion and molecular weight of small molecules by direct fitting of sedimentation velocity concentration profiles. Schester T.M, Laue T.M. Modern Analytical Ultracentrifugation. 1994;156-170 Birkäuser, Boston.
12. Laue T.M., Ridgeway T.M., Pelletier S. Computer-aided interpretation of analytical sedimentation data for proteins. Harding S.E, Rowe A.J, Horton J.C. Analytical Ultracentrifugation in Biochemistry and Polymer Science. 1992;90-125 Redwood, Melksham, Wiltshire.
13. Stafford W.F.D. Boundary analysis in sedimentation transport experiments: a procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile. Anal. Biochem. 203:1992;295-301.
14. DeVendittis E., Palumbo G., Parlato G., Bocchini V. A fluorimetric method for the estimation of the critical micelle concentration of surfactants. Anal. Biochem. 115:1981;278-286.
15. Timmins P.A., Leonhard M., Weltzien H.U., Wacker T., Welte W. A physical characterization of some detergents of potential use for membrane protein crystallization. FEBS Lett. 238:1988;361-368.
16. Van DenBoomgaard T., Zourab S.M., Lyklema J. On the influence of concentrated electrolytes on the association behavior of a non-ionic surfactant. Progr. Colloid Polym. Sci. 68:1983;25-32.
17. Lin T.H., Quinn T., Walsh M., Grandgenett D., Lee J.C. Avian myeloblastosis virus reverse transcriptase. Effect of glycerol on its hydrodynamic properties. J. Biol. Chem. 266:1991;1635-1640.