Defining a novel 75-kDa phosphoprotein associated with SS-A/Ro and identification of distinct human autoantibodies

Journal of Clinical Investigation

Volumn 104, Issue 9, 1999, Pages 1265-1275

  Wang, Dunrui ^a   Buyon, Jill P ^b,c   Zhu, Weiguo ^a   Chan, Edward K L ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NYU HOSPITAL FOR JOINT DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIBODY; AUTOANTIGEN; PHOSPHOPROTEIN;

ANTIBODY DETECTION; ANTIGEN BINDING; ANTIGEN RECOGNITION; ARTICLE; CROSS LINKING; HEART; HEART BLOCK; HUMAN; HUMAN CELL; LUPUS ERYTHEMATOSUS; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SJOEGREN SYNDROME;

EID: 0032750834     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI8003     Document Type: Article

References (41)

1. Buyon, J.P., et al. 1993. Identification of mothers at risk for congenital heart block and other neonatal lupus syndromes in their children. Comparison of enzyme-linked immunosorbent assay and immunoblot for measurement of anti-SS-A/Ro and anti-SS-B/La antibodies. Arthritis Rheum. 36:1263-1273.
2. Buyon, J.P. 1996. Neonatal lupus. Curr. Opin Rheum. 8:485-490.
3. Silverman, E., Mamula, M., Hardin, J.A., and Laxer, R. 1991. Importance of the immune response to the Ro/La particle in the development of congenital heart block and neonatal lupus erythematosus. J. Rheumatol. 18:120-124.
4. Reichlin, M., et al. 1994. Concentration of autoantibodies to native 60-kD Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block. Arthritis Rheum. 37:1698-1703.
5. von Muhlen, C.A., and Tan, E.M. 1995. Autoantibodies in the diagnosis of systemic rheumatic diseases. Semin. Arthritis Rheum. 24:323-358.
6. Ben-Chetrit, E., et al. 1988. A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J. Exp. Med. 167:1560-1571.
7. Wolin, S.L., and Steitz, J.A. 1983. Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome. Cell. 32:735-744.
8. Wolin, S.L., and Steitz, J.A. 1984. The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on theRo RNAs. Proc. Natl. Acad. Sci. USA. 81:1996-2000.
9. Pruijn, G.J., Simons, F.H., and van Venrooij, W.J. 1997. Intracellular localization and nucleocytoplasmic transport of Ro RNP components. Eur. J. Cell Biol. 74:123-132.
10. Ben-Chetrit, E., Gandy, B.J., Tan, E.M., and Sullivan, K.F. 1989. Isolation and characterization of a cDNA clone encoding the 60-kD component of the human SS-A/Ro ribonucleoprotein autoantigen. J. Clin. Invest. 83:1284-1292.
11. Deutscher, S.L., Harley, J.B., and Keene, J.D. 1988. Molecular analysis of the 60-kDa human Ro ribonucleoprotein. Proc. Natl. Acad. Sci. USA. 85:9479-9483.
12. Labbe, J.C., Jannatipour, M., and Rokeach, L.A. 1995. The Caenorhabditis elegant rop-1 gene encodes the homologue of the human 60-kDa Ro autoantigen. Gene. 167:227-231.
13. O'Brien, C.A., Margelot, K., and Wolin, S.L. 1993. Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins. Proc. Natl. Acad. Sci. USA. 90:7250-7254.
14. Wang, D., Buyon, J.P., and Chan, E.K.L. 1996. Cloning and expression of mouse 60 kDa ribonucleoprotein SS-A/Ro. Mol. Biol. Rep. 23:205-210.
15. Farris, A.D., Puvion-Dutilleul, F., Puvion, E., Harley, J.B., and Lee, L.A. 1997. The ultrastructural localization of 60-kDa Ro protein and human cytoplasmic RNAs: association with novel electron-dense bodies. Proc. Natl. Acad. Sci. USA. 94:3040-3045.
16. O'Brien, C.A., and Wolin, S.L. 1994. A possible role for the 60-kD Ro autoantigen in a discard pathway for defective SS rRNA precursors. Genes Dev. 8:2891-2903.
17. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY. 2.108-2.107.
18. Altschul, S.F., et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
19. ExPASy Proteomics. http://www.expasy.ch/www/tools.html. Accessed July, 1999.
20. de Wet, J.R., Wood, K.V., DeLuca, M., Helinski, D.R., and Subramani, S. 1987. Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell Biol. 7:725-737.
21. Chan, E.K.L., Hamel, J.C., Buyon, J.P., and Tan, E.M. 1991. Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen. J. Clin. Invest. 87:68-76.
22. Chan, E.K.L., Imai, H., Hamel, J.C., and Tan, E.M. 1991. Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor. J. Exp. Med. 173:1239-1244.
23. Chan, E.K.L., Francoeur, A.M., and Tan, E.M. 1986. Epitopes, structural domains, and asymmetry of amino acid residues in SS-B/La nuclear protein. J. Immunol. 136:3744-3749.
24. Laemmli, U.K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature. 227:680-685.
25. Wang, D., and Chan, E.K.L. 1996.17-beta-estradiol increases expression of the 52 and 60-kD SS-A/Ro autoantigens in human keratinocytes and breast cancer cell line MCF-7. J. Invest. Dermatol. 107:610-614.
26. Goblet, C., Prost, E., and Whalen, R.G. 1989. One-step amplification of transcripts in total RNA using the polymerase chain reaction. Nucleic Acids Res. 17:2144.
27. Chan, E.K.L, and Pollard, K.M. 1997. Detection of autoantibodies to ribonucleoprotein particles by immunoblotting. In Manual of clinical laboratory immunology. N.R. Rose, E.C. de Macario, J.D. Folds, H.C. Lane, and R.M. Nakamura, editors. American Society of Microbiology. Washington, DC. 928-934.
28. Rubin, R.L. 1997. Enzyme-linked immunosorbent assay for antibodies to native DNA, histones and (H2A-H2B)-DNA. In Manual of clinical laboratory immunology. N.R. Rose, E.C. de Macario, J.D. Folds, H.C. Lane, and R.M. Nakamura, editors. American Society of Microbiology. Washington, DC. 935-941.
29. Buyon J.P., et al. 1998. Autoimmune-associated congenital heart block: demographics, mortality, morbidity and recurrence rates obtained from a national neonatal lupus registry. J. Am. Coll. Cardiol. 31:1658-66.
30. Nagase, T., et al. 1998. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5:355-364.
31. Kozak, M. 1987. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:8125-8148.
32. Chan, E.K.L. and Buyon, J.P. 1994. The SS-A/Ro antigen. In Manual of biological markers of disease. W.J. van Venrooij and R.N. Maini, editors. Kluwer Academic Publishers. Dordrecht, the Netherlands. 1-18.
33. Tan, E.M., Chan, E.K.L., Sullivan, K.F., and Rubin, R.L 1988. Antinuclear antibodies (ANAs): diagnostically specific immune markers and clues toward the understanding of systemic autoimmunity. Clin. Immunol. Immunopathol. 47:121-141.
34. Reichlin, M. 1995. Autoantibodies to the RoRNP particles. Clin. Exp. Immunol. 99:7-9.
35. Horsfall, A.C., Venables, P.J., Taylor, P.V., and Maini, R.N. 1991. Ro and La antigens and maternal anti-La idiotype on the surface of myocardial fibres in congenital heart block. J. Autoimmun. 4:165-176.
36. Garcia, S., et al. 1994. Cellular mechanism of the conduction abnormalities induced by serum from anti-Ro/SSA-positive patients in rabbit hearts. J. Clin. Invest. 93:718-724.
37. Boutjdir, M., et al. 1997. Arrhythmogenicity of IgG and anti-52-kD SSA/Ro affinity-purified antibodies from mothers of children with congenital heart block. Circ. Res. 80:354-362.
38. Ramos-Deleon, M., and Herrera-Esparza, R. 1998. Tyrosine kinase participates in phosphorylation of the Ro60 ribonucleoprotein. Rev. Rhum. Engl. Ed. 65:89-93.
39. Topfer, F., Gordon, T., and McCluskey, J. 1995. Intra-and intermolecular spreading of autoimmunity involving the nuclear self-antigens La (SS-B) and Ro (SS-A). Proc. Natl. Acad. Sci. USA. 92:875-879.
40. Keech, C. L., Gordon, T. P., and McCluskey, J. 1996. The immune response to 52-kDa Ro and 60-kDa Ro is linked in experimental autoimmunity. J. Immunol. 157:3694-3699.
41. Tseng, C.E., et al. 1997. The 52-kD protein as a target of intermolecular spreading of the immune response to components of the SS-A/Ro-SS-B/La complex. Arthritis Rheum. 40:936-944.