H+/solute-induced intracellular acidification leads to selective activation of apical Na+/H+ exchange in human intestinal epithelial cells

Journal of Clinical Investigation

Volumn 104, Issue 5, 1999, Pages 629-635

  Thwaites, David T ^a   Ford, Dianne ^a   Glanville, Michael ^a   Simmons, Nicholas L ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; SODIUM ION;

ACIDIFICATION; ARTICLE; CELL PH; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; INTESTINE EPITHELIUM; MEMBRANE TRANSPORT; PRIORITY JOURNAL; PROTON SODIUM EXCHANGE; SEQUENCE ANALYSIS;

EID: 0032723028     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI7192     Document Type: Article

References (32)

1. + symporters. J. Membr. Biol. 154:197-203.
2. Leibach, F.H., and Ganapathy, V. 1996. Peptide transporters in the intestine and the kidney. Annu. Rev. Nutr. 16:99-119.
3. Hoshi, T., Takuwa, N., Abe, M., and Tajima, A. 1986. Hydrogen ion-coupled transport of D-glucose by phlorizin-sensitive sugar carrier in intestinal brush-border membranes. Biochim. Biophys. Acta. 861:483-488.
4. +/glucose cotransporter (SGLT1). J. Biol. Chem. 269:21407-21410.
5. +-coupled carriers at both apical and basal membranes. J. Biol. Chem. 268:7640-7642.
6. Thwaites, D.T., Cavet, M., Hirst, B.H., and Simmons, N.L. 1995. Angiotensin-converting enzyme (ACE) inhibitor transport in human intestinal epithelial (Caco-2) cells. Br. J. Pharmacol. 114:981-986.
7. +-coupled transepithelial β-alanine absorption by human intestinal Caco-2 cell monolayers. J. Biol. Chem. 268:18438-18441.
8. +-coupled amino acid transporter. Br. J. Pharmacol. 115:761-766.
9. McEwan, G.T.A., Daniel, H., Fett, C., Burgess, M.N., and Lucas, M.L. 1988. The effect of Escherichia coli STa enterotoxin and other secretagogues on mucosal surface pH of rat small intestine in vivo. Proc. R. Soc. Lond. B. 234:219-237.
10. Rawlings, J.M., Lucas, M.L., and Russel, R.I. 1987. Measurement of jejunal surface pH in situ by plastic pH electrode in patients with coeliac disease. Scand. J. Gastroenterol. 22:377-384.
11. Chu, S., and Montrose, M.H. 1995. Extracellular pH regulation in microdomains of colonic crypts: effects of short-chain fatty acids. Proc. Natl. Acad. Sci. USA. 92:3303-3307.
12. + exchange function is pliable in response ro transepithelial gradients of propionate. Proc. Natl. Acad Sci. USA. 91:6166-6170.
13. Thwaites, D.T., McEwan, G.T.A., and Simmons, N.L. 1995. The role of the proton electrochemical gradient in the transepithelial absorption of amino acids by human intestinal Caco-2 cell monolayers. J. Membr. Biol. 15:245-256.
14. + exchanger in renal microvillus membrane vesicles. Nature. 299:161-163.
15. Murer, H., Hopfer, U., and Kinne, R. 1976. Sodium/proton antiport in brush-border-membrane vesicles isolated from rat small intestine and kidney. Biochem. J. 154:597-604.
16. + exchangers of mammalian cells. J. Biol. Chem. 272:22373-22376.
17. + exchanger isoforms. Am. J. Physiol. 268:C283-C296.
18. + absorption in vivo. Gastroenterology. 112:174-183.
19. Walker, D., Thwaites, D.T., Simmons, N.L., Gilbert, H.J., and Hirst, B.H. 1998. Substrate up-regulation of the human small intestinal peptide transporter, hPepT1. J. Physiol. 507:697-706.
20. + exchanger isoforms NHE-1, NHE-2, and NHE-3 mRNA. Am. J. Physiol. 271:G483-G493.
21. + exchanger isoform, NHE3. Am. J. Physiol. 269:C198-C206.
22. + exchanger (SLC9A2). Genomics. 30:25-30.
23. + exchanger from Caco-2 cells, a human intestinal cell line. Am. J. Physiol. 261:G229-G238.
24. + exchangers stably expressed in a fibroblast cell line. J. Biol. Chem. 268:25527-25535.
25. + antiporter. Cell. 56:271-280.
26. +-coupled absorption. Br. J. Pharmacol. 113:1050-1056.
27. + transport in human jejunal brush-border membrane vesicles. Am. J. Physiol. 255:G206-G211.
28. Hoogerwerf, W.A., et al. 1996. NHE2 and NHE3 are human and rabbit intestinal brush-border proteins. Am. J. Physiol. 270:G29-G41.
29. Orlowski, J. 1993. Heterologous expression and functional properties of amiloride high affinity (NHE-1) and low affinity (NHE-3) isoforms of the rat Na/H exchanger. J. Biol. Chem. 268:16369-16377.
30. + exchanger. J. Gen. Physiol. 103:895-916.
31. + conductance. J. Gen. Physiol. 106:85-111.
32. + exchanger. Nat. Genet. 19:282-285.