Experimental spinal cord injury: Spatiotemporal characterization of elemental concentrations and water contents in axons and neuroglia

Journal of Neurophysiology

Volumn 82, Issue 5, 1999, Pages 2143-2153

  Lopachin, Richard M ^a,d   Gaughan, Christopher L ^a   Lehning, Ellen J ^a   Kaneko, Yoshiro ^b   Kelly, Thomas M ^c   Blight, Andrew ^c  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b NIHON UNIVERSITY   (Japan)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d MONTEFIORE MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CHLORIDE; ELEMENT; MAGNESIUM; MYELIN; PHOSPHORUS; SODIUM; TETRODOTOXIN; WATER;

ACTION POTENTIAL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GLIA; GUINEA PIG; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SPINAL CORD INJURY; X RAY MICROANALYSIS;

EID: 0032716249     PISSN: 00223077     EISSN: None     Source Type: Journal    
DOI: 10.1152/jn.1999.82.5.2143     Document Type: Article

References (63)

1. 2+ exchanger. J. Neurosci. 16: 545-552, 1996.
2. ALVAREZ-LEEFMANS, F. J., GIRALDEZ, F., AND GAMINO, S. M. Intracellular free magnesium in excitable cells: its measurement and its biologic significance. Can. J. Physiol. Pharmacol. 65: 915-925, 1987.
3. ANDERSON, D. K., MEANS, E. D., WATERS, T. R., AND SPEARS, C. J. Spinal cord energy metabolism following compression trauma to the feline spinal cord. J. Neurosurg. 53: 375-380, 1985.
4. BALENTINE, J. D. Pathology of experimental spinal cord trauma. II. Ultrastructure of axons and myelin. Lab Invest. 39: 254-256, 1978.
5. BANIK, N. L., HOGAN, E. L., AND HSU, C. Y. The multimolecular cascade of spinal cord injury. Neurochem. Path. 7: 57-77, 1987.
6. BERNARDI, P., BROEKEMEIER, K. M., AND PFEIFFER, D. R. Recent progress on regulation of the mitochondrial permeability transition pore: a cyclosporin-sensitive pore in the inner mitochondrial membrane. J. Bioenerg. Biomembr. 26: 509-517, 1994.
7. BLIGHT, A. R. Effect of 4-aminopyridine on axonal conduction-block in chronic spinal cord injury. Brain Res Bull. 22: 47-52, 1989.
8. BLIGHT, A. R. Morphometric analysis of a model of spinal cord injury in guinea pigs, with behavioral evidence of delayed secondary pathology. J. Neurol. Sci. 103: 156-171, 1991.
9. BLIGHT, A. R. Remyelination, revascularization, and recovery of function in experimental spinal cord injury. In: Advances in Neurology, edited by F. J. Seil. New York: Raven, 1993, p. 91-104.
10. BLIGHT, A. R. AND DECRESCITO, V. Morphometric analysis of experimental spinal cord injury in the cat: the relation of injury intensity to survival of myelinated axons. Neuroscience 19: 321-341, 1986.
11. BRESNAHAN, J. C. An electron-microscopic analysis of axonal alternations following blunt contusion of the spinal cord of the rhesus monkey. J. Neurol. Sci. 37: 59-82, 1978.
12. BULGER, R. E., BEEUWKES, R., AND SAUBERMANN, A. J. Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. III. Elemental content of cells in the rat renal papillary tip. J. Cell Biol. 88: 233-238, 1981.
13. + following contusion of the rat spinal cord. Brain Res. 556: 71-77, 1991.
14. + following graded contusion of the rat spinal cord. Exp. Neurol. 125: 93-98, 1994.
15. CLENDENON, N. R., ALLEN, N., GORDON, W. A., AND BINGHAM, W. G. Inhibition of Na, K-activated ATPase activity following experimental spinal cord trauma. J. Neurosurg. 49: 563-568, 1978.
16. DEMEDIUK, P., SAUNDERS, R. D., ANDERSON, D. K., MEANS, E. D., AND HORROCKS, L. A. Early membrane lipid changes in laminectomized and traumatized cat spinal cord. Neurochem. Pathol. 7: 79-89, 1987.
17. DOHRMANN, G. J., WAGNER, F. C., AND BUCY, P. C. Transitory traumatic paraplegia: electron microscopy of early alterations in myelinated nerve fibers. J. Neurosurg. 36: 407-415, 1972.
18. FADEN, A. I., CHAN, P. H., AND LONGAR, S. Alterations in lipid metabolism, Na, K-ATPase activity, and tissue water content of spinal cord following experimental traumatic injury. J. Neurochem. 48: 1809-1816, 1987.
19. FEHLINGS, M. G. AND NASHMI, R. Assessment of axonal dysfunction in an in vitro model of acute compressive injury to adult rat spinal cord axons. Brain Res. 677: 291-299, 1995.
20. FEHLINGS, M. G. AND TATOR, C. H. The relationships among the severity of spinal cord injury, residual neurological function, axon counts, and counts of retrogradely labeled neurons after experimental spinal cord injury. Exp. Neurol. 132: 220-228, 1995.
21. FOSTER, M. C. AND SAUBERMANN, A. J. Low cost system for electron probe microanalysis based on a personal computer. J. Microsc. 161: 367-373, 1991.
22. GOLDMAN, S. S., ELOWITZ, E., AND FLAMM, E. S. Effect of traumatic injury on membrane phosphatase activity in cat spinal cord. Exp. Neurol. 82: 650-662, 1983.
23. GREENE, W. B. AND WALSH, L. G. Cryo-jet preservation of calcium in the rat spinal cord. Scan. Microsc. 8: 587-599, 1995.
24. GRUNER, J. A., YEE, A. K., AND BLIGHT, A. R. Histological functional evaluation of experimental spinal cord injury: evidence of a stepwise response to graded compression. Brain Res. 729: 90-101, 1996.
25. GUENTER, T. D., GUNTER, K. K., SHEU, S. S., AND GAVIN, C. E. Mitochondrial calcium transport: physiological and pathological relevance. Am. J. Physiol. 267 (Cell Physiol. 36): C313-C339, 1994.
26. HALL, T. A., ANDERSON, H. C., AND APPLETON, T. The use of thin specimens for x-ray microanalysis in biology. J. Microsc. 99: 177-182, 1973.
27. HALL, E. D. AND BRAUGHLER, J. M. Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for the involvement of oxygen radicals and lipid peroxidation. Free Radic. Biol. Med. 6: 303-313, 1989.
28. HAPPEL, R. D., SMITH, K. P., BANIK, N. L., POWERS, J. M., HOGAN, E. L., AND BALENTINE, J. D. Ca-accumulation in experimental spinal cord trauma. Brain Res. 211: 476-479, 1981.
29. HONMOU, O. AND YOUNG, W. Traumatic injury of spinal axons. In: The Axon, edited by S. G. Waxman, J. D. Kocsis, and P. K. Stys. New York: Oxford Univ. Press, 1995, p. 480-503.
30. KURIHARA, M. Role of monoamines in experimental spinal cord injury in rats: relationship between Na-K ATPase and lipid peroxidation. J. Neurosurg. 62: 743-749, 1985.
31. KWO, S., YOUNG, W., AND DECRESCITO, V. Spinal cord sodium, potassium, calcium, and water concentration changes in rats after graded contusion injury. J. Neurotrauma 6: 13-24, 1989.
32. + and spinal cord edema following impact trauma in rats. Biochem. Biophys. Res. Commun. 147: 1170-1175, 1987.
33. LOPACHIN, R. M. Electron probe X-ray microanalysis as a tool for discerning mechanisms of nerve injury. In: Neurotoxicology: Approaches and Methods, edited by L. W. Chang and W. Slikker. San Diego, CA: Academic, 1995, p. 445-453.
34. LOPACHIN, R. M., CASTIGLIA, C., AND SAUBERMANN, A. J. Perturbation of axonal elemental composition and water content: implications for neurotoxic mechanisms. Neurotoxicology 13: 123-138, 1992a.
35. LOPACHIN, R. M., CASTIGLIA, C. M., AND SAUBERMANN, A. J. Elemental composition and water content of myelinated axons and glial cells in rat central nervous system. Brain Res. 549: 253-259, 1991.
36. LOPACHIN, R. M., CASTIGLIA, C. M., AND SAUBERMANN, A. J. Acrylamide disrupts elemental composition and water content of rat tibial nerve. I. Myelinated axons. Toxicol. Appl. Pharmacol. 115: 21-34, 1992b.
37. LOPACHIN, R. M. AND LEHNING, E. J. Acrylamide-induced distal axon degeneration: a proposed mechanism of action. Neurtoxicology 15: 247-260, 1994.
38. LOPACHIN, R. M. AND LEHNING, E. J. Mechanism of calcium entry during axon injury and degeneration. Toxicol. Appl. Pharmacol. 143: 233-244, 1997.
39. LOPACHIN, R. M., LOPACHIN, V., AND SAUBERMANN, A. J. Effects of axotomy on distribution and concentration of elements in rat sciatic nerve. J. Neurochem. 54: 320-332, 1990.
40. LOPACHIN, R. M., LOWERY, J., EICHBERG, J., KIRKPATRICK, J. B., CARTWRIGHT, J., AND SAUBERMANN, A. J. Distribution of elements in rat peripheral axons and nerve cell bodies determined by x-ray microprobe analysis. J. Neurosci. 51: 764-775, 1988.
41. LOPACHIN, R. M. AND STYS, P. K. Elemental composition and water content of rat optic nerve myelinated axons and glial cells: effects of in vitro anoxia and reoxygenation. J. Neurosci. 15: 6735-6746, 1995.
42. MORIYA, T., HASSAN, A. Z., YOUNG, W., AND CHESLER, M. Dynamics of extracellular calcium activity following contusion of the rat spinal cord. J. Neurotrauma 11: 255-263, 1994.
43. OCHS, S., POURMAND, R., JERSILD, R. A., AND FRIEDMAN, R. N. The origin and nature of beading: a reversible transformation of the shape of nerve fibers. Prog. Neurobiol. 52: 391-426, 1997.
44. RANSOM, B. R. AND ORKAND, R. K. Glial-neuronal interactions in non-synaptic areas of the brain: studies in the optic nerve. Trends Neurosci. 19: 352-358, 1996.
45. SAUBERMANN, A. J., BEEUWKES, R., AND PETERS, P. D. Application of scanning electron microscopy to X-ray analysis of frozen-hydrated sections. II. Analysis of standard solutions and artificial electrolyte gradients. J. Cell Biol. 88: 257-267, 1981a.
46. SAUBERMANN, A. J., ECHLIN, P., PETERS, P. D., AND BEEUWKES, R. Application of scanning electron microscopy to X-ray analysis of frozen-hydrated sections. I. Specimen handling techniques. J. Cell Biol. 88: 257-267, 1981b.
47. SAUBERMANN, A. J. AND HEYMAN, R. V. Quantitative digital x-ray imaging using frozen hydrated and frozen dried tissue sections. J. Microsc. 146: 169-182, 1987.
48. SAUNDERS, R. AND HORROCKS, L. A. Eicosanoids, plasma membranes, and molecular mechanisms of spinal cord injury. Neurochem. Pathol. 7: 1-22, 1987.
49. SHI, R. AND BLIGHT, A. R. Compression injury of mammalian spinal cord in vitro and the dynamics of action potential conduction failure. J. Neurophysiol. 76: 1572-1580, 1996.
50. STOKES, B. T., FOX, P., AND HOLLINDEN, G. Extracellular calcium activity in the injured spinal cord. Exp. Neurol. 80: 561-572, 1983.
51. STYS, P. K. Anoxic and ischemic injury of myelinated axons in CNS white matter: from mechanistic concepts to therapeutics. J. Cereb. Blood Flow Metab. 18: 2-25, 1998.
52. STYS, P. K. AND LOPACHIN, R. M. Mechanisms of calcium and sodium fluxes in anoxic myelinated central nervous system axons. Neuroscience 82: 21-32, 1998.
53. SYKOVA, E., SVOBODA, J., SIMONOVA, Z., AND JENDELOVA, P. Role of astrocytes in ionic and volume homeostasis in spinal cord during development and injury. In: Progress in Brain Research, edited by A.C.H. Yu, L. Hertz, M. D. Norenberg, E. Sykova, and S. G. Waxman. Amsterdam: Elsevier, 1992, vol. 94.
54. TAYLOR. C. P., WEBER, M. L., GAUGHAN, C. L., LEHNING, E. J., AND LOPACHIN, R. M. Oxygen/glucose deprivation in hippocampal slices: altered intraneuronal elemental composition predicts structural and functional damage. J. Neurosci. 19: 619-629, 1998.
55. TENG, Y. D. AND WRATHALL, J. R. Local blockade of sodium channels by tetrodotoxin ameliorates tissue loss and long-term functional deficits resulting from experimental spinal cord injury. J. Neurosci. 17: 4359-4366, 1997.
56. TRUMP, B. F., BEREZESKY, I. K., CHANG, S. H., PENDERGRASS, R. E., AND MERGNER, W. J. The role of ion shifts in cell injury. Scann. Elect. Microsc. III: 1-14, 1979.
57. 31P MRS. Brain Res. 490: 144-147, 1989.
58. WAXMAN, S. G., RANSOM, B. R., AND STYS, P. K. Non-synaptic mechanisms of Ca-mediated injury in CNS white matter. Trends Neurosci. 14: 461-468, 1991.
59. YOUNG, W. Role of calcium in central nervous system injuries. J. Neurotrauma 9: S9-S25, 1992.
60. YOUNG, W. Secondary mechanisms in acute spinal cord injury. J. Emerg. Med. 11: 13-22, 1993.
61. YOUNG, W. AND DECRESCITO, V. Sodium ionic changes in injured spinal cords: mechanisms of edema. Soc. Neurosci. Abstr. 16: 267, 1986.
62. YOUNG, W. AND KOREH, I. Potassium and calcium changes in injured spinal cords. Brain Res. 365: 42-53, 1986.
63. YOUNG, W., YEN, V., AND BLIGHT, A. Extracellular calcium ionic activity in experimental spinal cord contusion. Brain Res. 253: 105-113, 1982.