Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 18, Issue 9, 1999, Pages 643-647

  Jonas, D ^a   Grundmann, H ^b   Hartung, D ^a   Daschner, F D ^a   Towner, K ^b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b NOTTINGHAM UNIVERSITY HOSPITALS NHS TRUST   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

METICILLIN; OXACILLIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM IDENTIFICATION; COAGULASE NEGATIVE STAPHYLOCOCCUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GERMANY; HUMAN; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS INFECTION; TIME;

BACTERIAL PROTEINS; CARRIER PROTEINS; HEXOSYLTRANSFERASES; METHICILLIN RESISTANCE; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PENICILLIN-BINDING PROTEINS; PEPTIDYL TRANSFERASES; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

EID: 0032708729     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s100960050365     Document Type: Article

References (31)

1. 1. Speller DC, Johnson AP, James D, Marples RR, Charlett A, George RC: Resistance to methicillin and other antibiotics in isolates of Staphylococcus aureus from blood and cerebro-spinal fluid, England and Wales, 1989-95. Lancet (1997) 350:323-325
2. 2. Humphreys H, Duckworth G: Methicillin-resistant Staphylococcus aureus (MRSA) - a re-appraisal of control measures in the light of changing circumstances. Journal of Hospital Infection (1997) 36:167-170
3. 3. Voss A, Milatovic D, Wallrauch-Schwarz C, Rosdahl VT, Braveny I: Methicillin-resistant Staphylococcus aureus in Europe. European Journal of Clinical Microbiology & Infectious Diseases (1994) 13:50-55
4. 4. Schmilz FJ, MacKenzie CR, Geisel R, Wagner S, Idel H, Verhoef J, Hadding U, Heinz HP: Methicillin resistant Staphylococcus aureus strains in the greater Dusseldorf area. European Journal of Epidemiology (1997) 13:709-717
5. 5. Barski P, Piechowicz L, Galinski J, Kur J: Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR. Molecular and Cellular Probes (1996) 10:471-475
6. 6. Schmitz FJ, Mackenzie CR, Hofmann B, Verhoef J, Finken-Eigen M, Heinz HP, Kohrer K: Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR. Journal of Medical Microbiology (1997) 46:773-778
7. 7. Vannuffel P, Gigi J, Ezzedine H, Vandercam B, Delmee M, Wauters G, Gala JL: Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. Journal of Clinical Microbiology (1995) 33:2864-2867
8. 8. Kobayashi N, Wu H, Kojima K, Taniguchi K, Urasawa S, Uehara N, Omizu Y, Kishi Y, Yagihashi A, Kurokawa I: Detection of mecA, femA and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidemiology and Infection (1994) 113:259-266
9. 9. Towner KJ, Talbot DCS, Curran R, Webster CA, Humphreys H: Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology (1998) 47:1-7
10. 10. National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A4. NCCLS, Villanova, PA (1997)
11. 11. Bauer AW, Kirby WM, Sherris JC, Turck M: Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology (1966) 45:493-496
12. 12. Geha DJ, Uhl JR, Gustaferro CA, Persing DH: Multiplex PCR of identification of methicillin-resistant staphylococci in the clinical laboratory. Journal of Clinical Microbiology (1994) 32:1768-1772
13. 13. Grundmann HJ, Towner KJ, Dijkshoorn L, Gerner-Smidt P, Maher M, Seifert H, Vaneechoutte M: Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp. Journal of Clinical Microbiology (1997) 35:3071-3077
14. 14. Kumari DNP, Peer V, Hawkey PM, Parnell P, Joseph N, Richardson JF, Cookson B: Comparison and application of ribosome spacer DNA amplicon polymorphisms and pulsed-field gel electrophoresis for differentiation of methicillin-resistant Staphylococcus aureus strains. Journal of Clinical Microbiology (1997) 35:881-885
15. 15. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research (1997) 25:3389-3402
16. 16. Ryffel C, Kayser FH, Berger-Bachi B: Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci. Antimicrobial Agents and Chemotherapy (1992) 36:25-31
17. 17. Shay DK, Maloney SA, Montecalvo M, Banerjee S, Wormser GP, Arduino MJ, Bland LA, Jarvis WR: Epidemiology and mortality risk of vancomycin-resistant enterococcal blood-stream infections. Journal of Infectious Diseases (1995) 172:993-1000
18. 18. Hiramatsu K, Hanaki H, Ino T, Yabuta K, Oguri T, Tenover FC: Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. Journal of Antimicrobial Chemotherapy (1997) 40:135-136
19. 19. Centers for Disease Control and Prevention: Update: Staphylococcus aureus with reduced susceptibility to vancomycin -United States, 1997. Morbidity and Mortality Weekly Report (1997) 46:813-815
20. 20. Saruta K, Matsunaga T, Kono M, Hoshina S, Ikawa S, Sakai O, Machida K: Rapid identification and typing of Staphylococcus aureus by nested PCR amplified ribosomal DNA spacer region. FEMS Microbiology Letters (1997) 146:271-278
21. 21. Jensen MA, Webster JA, Straus N: Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied and Environmental Microbiology (1993) 59:945-952
22. 22. Brakstad OG, Aasbakk K, Maeland JA: Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Journal of Clinical Microbiology (1992) 30:1654-1660
23. 23. Berger-Bächi B, Strassle A, Gustafson JE, Kayser FH: Mapping and characterization of multiple chromosomal factors involved in methicillin resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy (1992) 36:1367-1373
24. 24. Unal S, Hoskins J, Flokowitsch JE, Wu CY, Preston DA, Skatrud PL: Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. Journal of Clinical Microbiology (1992) 30:1685-1691
25. 25. Kizaki M, Kobayashi Y, Ikeda Y: Rapid and sensitive detection of the femA gene in staphylococci by enzymatic detection of polymerase chain reaction (ED-PCR): comparison with standard PCR analysis. Journal of Hospital Infection (1994) 28:287-295
26. 26. Henze U, Sidow T, Wecke J, Labischinski H, Berger-Bachi B: Influence of femB on methicillin resistance and peptidoglycan metabolism in Staphylococcus aureus. Journal of Bacteriology (1993) 175:1612-1620
27. 27. Kloos WE: Staphylococcus. In: Collier L, Balows A, Sussman M (eds): Topley & Wilson's microbiology and microbial infections, Edward Arnold, London (1997) pp 578-632
28. 28. Akiyama H, Kanzaki H, Tada J, Arata J: Coagulase-negative staphylococci isolated from various skin lesions. Journal of Dermatology (1998) 25:563-568
29. 29. Frebourg NB, Nouet D, Lemee L, Martin E, Lemeland JF: Comparison of ATB staph, rapid ATB staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA. Journal of Clinical Microbiology (1998) 36:52-57
30. 30. Kolbert CP, Arruda J, Varga-Delmore P, Zheng X, Lewis M, Kolberg J, Persing DH: Branched-DNA assay for detection of the mecA gene in oxacillin-resistant and oxacillin-sensitive staphylococci. Journal of Clinical Microbiology (1998) 36:2640-2644
31. 31. Edwards MC, Gibbs RA: Multiplex PCR. In: Dieffenbach CW, Dveksler GS (eds): PCR primer: a laboratory manual. Cold Spring Harbor Laboratory Press, New York (1995) pp 157-171