Rapid identification and typing of Staphylococcus aureus by nested PCR amplified ribosomal DNA spacer region

FEMS Microbiology Letters

Volumn 146, Issue 2, 1997, Pages 271-278

  Saruta, Katsutoshi ^a   Matsunaga, Teiichi ^a   Kono, Midori ^a   Hoshina, Sadayori ^a   Ikawa, Sachio ^a   Sakai, Osamu ^a   MacHida, Katsuhiko ^a  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

nested PCR amplification; ribosomal DNA spacer region; Staphylococcus aureus

Indexed keywords

RIBOSOME DNA; SPACER DNA;

ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS;

DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SPECIES SPECIFICITY; STAPHYLOCOCCUS AUREUS;

EID: 0031028847     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00487-9     Document Type: Article

References (13)

1. Greisen, K., Loeffelholz, M., Purohit, A. and Leong, D. (1994) PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Clin. Microbiol. 32, 335-351.
2. Jensen, M.A., Webster, J.A. and Straus, N. (1993) Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol. 59, 945-952.
3. Relman, D.A. (1993) The identification of uncultured microbial pathogens (review). J. Infect. Dis. 168, 1-8.
4. Machida, K. and Hoshina, S. (1992) Clinical detection of bacterial DNA (review). Igakunoayumi 162, 527-531.
5. Saruta, K., Kono, M., Hoshina, S., Kanemoto, S., Ikawa, S., Sakai, O. and Machida, K. (1996) Rapid detection and taxonomic identification of bacteria by two-step polymerase chain reaction. Jikei Med. J. 43, 95-103.
6. Saruta, K., Hoshina, S. and Machida, K. (1995) Genetic identification of Staphylococcus aureus by polymerase chain reaction using single-base-pair mismatch in 16S ribosomal RNA gene. Microbiol. Immunol. 39, 839-844.
7. Saruta, K., Hoshina, S. and Machida, K. (1996) Clinical detection of bacterial DNA (review). Media-circle 41, 341-351 (in Japanese).
8. Saruta, K., Matsunaga, T., Hoshina, S., Kono, M., Kitahara, S., Kanemoto, S., Sakai, O. and Machida, K. (1995) Rapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region. FEMS Lett. 132, 165-170.
9. Saruta, K. (1996) A new simplified clarification for epidemiological study of the Staphylococcus aureus infection. Tokyo Jikeikai Ikadaigaku Zasshi 111, 227-234.
10. Prevost, G., Jaulhac, B. and Piemont, Y. (1992) DNA fingerprinting by pulsed field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J. Clin. Microbiol. 30, 967-973.
11. Blair, J.E. and Williams, R.E.O. (1961) Phage typing of staphylococci. Bull. WHO 24, 771-784.
12. Japan Society of Chemotherapy (1981) Methods for dilution of antimicrobial susceptibility tests for bacteria. Jpn. J. Chemother. 29, 76-79 (in Japanese).
13. Kiss, A., Sain, B. and Venetianer, P. (1977) The number of rRNA genes in Escherichia coli. FEMS Lett. 79, 77-79.