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7 Speiser DE, Tiercy J-M, Rufer N, Grundschober C, Gratwohl A, Chapuis B, et al: High resolution HLA matching associated with decreased mortality after unrelated bone marrow transplantation. Blood 1996, 87:4455-4462.
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Effect of matching of class I HLA alleles on clinical outcome after transplantation of hematopoietic stem cells from an unrelated donor. Japan marrow donor program
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8 Sasazuki T, Juji T, Morishima Y, Kinukawa N, Kashiwabara H, Inoko H, et al.: Effect of matching of class I HLA alleles on clinical outcome after transplantation of hematopoietic stem cells from an unrelated donor. Japan Marrow Donor Program. N Engl J Med 1998, 339:1177-1185. This is an analysis of the effect of HLA class I and class II mismatches (high-resolution typing) on the outcome of 440 transplantations with unrelated stern cell donors. The 1-year survival rate of fully matched HLA-AI-BI-CI-DRB1 patients was 65%. Multivariate analysis showed that only HLA-A mismatches correlated with lower survival. In contrast, neither HLA-BI-C nor HLA-DRBI-DQ mismatches appeared to influence survival, although the univariate analysis showed an effect of HLA-B.
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9 Petersdorf EW, Gooley TA, Anasetti C, Martin PJ, Smith AG, Mickelson EM, et al.: Optimizing outcome after unrelated marrow transplantation by comprehensive matching of HLA class I and II alleles in the donor and recipient. Blood 1998, 92:3515-3520. This is a multivariate analysis of the effect of HLA class I and class II mismatches (high-resolution typing) in a group of 300 chronic myeloid leukemia patients transplanted with stem cells from an unrelated donor. The article shows that mismatching for a single class I or class II allele had little effect on survival. Only a combination of two mismatches increased mortality. Overall 5-year survival of fully matched pairs was at 56%.
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Petersdorf, E.W.1
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10 Hansen JA, Gooley TA, Martin PJ, Appelbaum F, Chauncey TR, Clift RA, et al.: Bone marrow transplants from unrelated donors for patients with chronic myeloid leukemia. N Engl J Med 1998, 338:962-968. These authors present the clinical outcome of 196 chronic myeloid leukemia patients in the chronic phase who received unrelated stem cell transplants between 1985 and 1994. Kaplan-Meier estimate of 5-year survival was 74% for patients under 50 years of age who underwent transplantation during the first year after diagnosis with marrow from an ABDR-matched donor. Donor/recipient pairs were HLA-AI-B typed by serology and DNA-typed for HLA-DRB1. The article shows that HLA-DRB1 mismatches increased mortality, whereas HLA-AI -B mismatches did not. However, these results should be interpreted with caution because HLA-DRB1-mismatched pairs are more often mismatched for class I alleles not detected by serology than HLA-DRB1-matched combinations.
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16 Rufer N, Tiercy J-M, Breur-Vriesendorp B, Gauchat-Feiss D, Shi X, Slavcev A, et al.: Histoincompatibilities in ABDR-matched unrelated donor recipient combinations. Bone Marrow Transplant 1995, 16:641-646.
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17 Prasad VK, Kernan NA, Heller G, O'Reilly RJ, Yang SY: DNA typing for HLA-A and HLA-B identifies disparities between patients and unrelated donors matched by HLA-A and HLA-B serology and HLA-DRB1. Blood 1999, 93:399-409. These authors describe large-scale HLA-AI-B sequence-specific oligonucleotide typing of 128 patients and their 484 potential donors matched for HLA-AB by serology and for HLA-DRB1 by DNA typing. The information on the level of mismatches for certain AB-serotypes is very useful to determine the class I DNA-typing strategy.
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18 Olerup O, Zetterquist H: HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992, 39:225-235.
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Phototyping: Comprehensive DNA typing for HLA-A, B, C, DRB1, DRB1, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP)
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19 Bunce M, O'Neill CM, Barnardo MC, Krausa P, Browning MJ, Morris PJ, Welsh KI: Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB1, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP). Tissue Antigens 1995, 46:355-367.
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20 Albis-Camps M, Blasczyk R: Fluorotyping of HLA-DRB by sequence-specific priming and fluorogenic probing. Tissue Antigens 1999, 53:301-307. The authors describe a sequence-specific primer technique for HLA-DR typing in which a positive polymerase chain reaction is detected through a locus-specific fluorescent internal probe cleaved by the 5′-3′ exonuclease activity of the Taq polymerase. This technique, previously reported by the same group for HLA-AI-C could help sequence-specific primer automation substantially.
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29 Turner S, Ellexson ME, Hickman HD, Sidebottom DA, Fernandez-Vina M, Confer DL, et al.: Sequence-based typing provides a new look at HLA-C diversity. J Immunol 1998, 161:1406-1413. This is an analysis of HLA-C locus diversity by sequencing the HLA-C alleles of 1823 donors in the NMDP Registry. In total, 48 different sequences were found. The frequency of new sequences, identified in approximately 1% of the individuals, was significantly higher in non-Caucasian patients, which has practical consequences for the routine typing strategy.
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32 Argüello JR, Little AM, Pay AL, Gallardo D, Rojas I, Marsh SG, et al: Mutation detection and typing of polymorphic loci through double-strand conformation analysis. Nat Genet 1998, 18:192-194. These authors describe a method of detecting genetic polymorphisms based on characteristic mobilities of heteroduplex DNA fragments in polyacrylamide gels. The heteroduplexes are formed by reannealing the polymerase chain reaction-amplified fragment of the sequence to be tested with a fluorescein-labeled fragment of one of the alleles of the locus tested. After electrophoresis, the migration of all heteroduplexes formed are measured on an automated DNA sequencer. The authors describe the unique mobilities of 131 HLA class I alleles.
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34 Argüello JR, Little AM, Bohan E, Gallardo D, O'Shea J, Dodi IA, et al.: A high resolution HLA class I and class II matching method for bone marrow donor selection. Bone Marrow Transplant 1998, 22:527-534. These authors cover the application of the reference strand-mediated conformation analysis described in references 32 and 33 for compatibility testing of 120 donors selected for 48 patients. The article shows that the method detects sero-logically hidden subtypes and that within 12 hours, multiple donor samples can be processed simultaneously.
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Measurement of cytotoxic T lymphocyte precursor frequencies reveals cryptic HLA class I mismatches in the context of unrelated donor bone marrow transplantation
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37 Shea JO, Madrigal A, Davey N, Brookes P, Scott I, Firman H, et al.: Measurement of cytotoxic T lymphocyte precursor frequencies reveals cryptic HLA class I mismatches in the context of unrelated donor bone marrow transplantation. Transplantation 1997, 64:1353-1356.
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