Rapid assembly of synthetic genes encoding protein polymers

Macromolecules

Volumn 32, Issue 11, 1999, Pages 3643-3648

  McMillan, R Andrew ^a   Lee, Terrence A T ^a   Conticello, Vincent P ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; DNA SEQUENCES; ENZYME KINETICS; MASS SPECTROMETRY; POLYPEPTIDES; PROTEINS;

ELASTIN MIMETIC POLYPEPTIDES; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; SEAMLESS CLONING TECHNIQUE; SYNTHETIC GENES;

CLONING;

EID: 0032675950     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma981660f     Document Type: Article

References (44)

1. (a) Heslot, H. Biochimie 1998, 80, 19-31.
2. (b) Ferrari, F. A.; Cappello, J. In Protein-Based Materials; McGrath, K., Kaplan, D., Eds.; Birkhauser: Boston, 1997; pp 37-60.
3. (c) Cappello, J.; Ferrari, F. In Plastics from Microbes: Microbial Synthesis of Polymers and Polymer Precursors; Mobley, D. P., Ed.; Hanser/Gardner Publications: Munich, 1994; pp 35-92.
4. (d) Tirrell, J. G.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Chem. Eng. News 1994, 72, 40-51.
5. (e) O'Brien, J. P. Trends Polym. Sci. 1993, 1, 228-232.
6. Hudson, S. M. In Protein-Based Materials; McGrath, K., Kaplan, D., Eds.; Birkhauser: Boston, 1997; pp 313-337.
7. Deming, T. J. Adv. Mater. 1997, 9, 299-311.
8. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
9. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
10. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
11. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
12. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
13. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
14. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
15. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
16. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
17. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
18. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
19. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
20. For leading references in the biosynthesis of repetitive polypeptides based on natural protein materials, please see the following: (a) Elastin: McPherson, D. T.; Xu, J.; Urry, D. W. Protein Expr. Purif. 1996, 7, 51-7. McPherson, D. T.; Morrow, C.; Minehan, D. S.; Wu, J.; Hunter, E.; Urry, D. W. Biotechnol. Prog. 1992, 8, 347-352. (b) N. clavipes dragline silk: Prince, J. T.; McGrath, K. P.; DiGirolamo, C. M.; Kaplan, D. L. Biochemistry 1995, 34, 10879-10885. Fahnestock, S. R.; Irwin, S. L. Appl. Microbiol. Biotechnol. 1997, 47, 23-38. Fahnestock, S. R.; Bedzyk, L. A. Appl. Microbiol. Biotechnol. 1997, 47, 33-39. Lewis, R. V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Protein Exp. Purif. 1996, 7, 400-406. (c) M. edulis mussel adhesive: Salerno, A. J.; Goldberg, I. Appl. Microbiol. Biotechnol. 1993, 39, 221-226. Masiliamani, D.; Goldberg, I.; Salerno, A. J.; Oleksiuk, M. A.; Unger, P. D.; Piascik, D. A.; Bhattacharjee, H. R. In Biotechnology and Polymers; Gebelein, C. G., Ed.; Plenum Press: New York, 1991; pp 245-264. Strausberg, R. L.; Anderson, D. M.; Filpula, D.; Finkelman, M.; Link, R.; McCandliss, R.; Orndorff, S. A.; Strausberg, S. L.; Wei, T. In Adhesives From Renewable Resources; Hemingway, R. W., Conners, A. H., Branham, S. J., Eds.; ACS Symposium Series 385; American Chemical Society: Washington, DC, 1989; pp 453-483. (d) Collagen: Goldberg, I.; Salerno, A. J.; Patterson, T.; Williams, J. I. Gene 1989, 80, 305-314. (e) Glutenin: Anderson, O. D.; Kuhl, J. C.; Tam, A. Gene 1996, 174, 51-58. (f) B. mori silk fibroin: Cappello, J.; Crissman, J. W.; Dorman, M.; Mikolajczak, M.; Textor, G.; Marquet, M.; Ferrari, F. Biotechnol. Prog. 1990, 6, 198-202. (g) Adenovirus fiber spike protein: O'Brien, J.; Hoess, R.; Gardner, K.; Lock, R.; Wasserman, Z.; Weber, P.; Salemme, F. In Silk Polymers-Materials Science and Biotechnology; Kaplan, D. L., Adams, W. W., Farmer, B. L., Viney, C., Eds.; American Chemical Society: Washington, DC, 1994; pp 104-117.
21. (a) Petka, W. A,; Harden, J. L.; McGrath, K. P.; Wirtz, D.; Tirrell, D. A. Science 1998, 281, 389-392.
22. (b) Yu, S. M.; Conticello, V. P.; Zhang, G.; Kayser, C.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Nature 1997, 389, 167-170.
23. (c) Krejchi, M. T.; Atkins, E. D. T.; Waddon, A. J.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Science 1994, 265, 1427-1432.
24. (d) Zhang, G.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Macromolecules 1992, 25, 3601-3603.
25. (e) McGrath, K. P.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. J. Am. Chem. Soc. 1992, 114, 727-733.
26. (f) Creel, H. S.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Macromolecules 1991, 24, 1213-1214.
27. Tirrell, D. A.; Fournier, M. J.; Mason, T. L. MRS Bull. 1991, 16, 23-28.
28. Studier, F. W.; Rosenberg, A. H.; Dunn, J. J.; Dubendorff, J. W. Methods Enzymol. 1989, 185, 60-89.
29. Panitch, A.; Matsuki, K.; Cantor, E. J.; Cooper, S. J.; Atkins, E. D. T.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A. Macromolecules 1997, 30, 42-49.
30. Padgett, K. A.; Sorge, J. A. Gene 1996, 168, 31-35.
31. Triglia, T.; Peterson, M. G.; Kemp, D. S. Nucleic Acids Res. 1988, 16, 8186.
32. A percentage of internal sites on the recipient plasmid will incorporate dCTP rather than 5-methyl-dCTP and will be cleaved by Eam 1104 I. Typically, the addition of 5-methyl-dCTP is delayed until the last five cycles of the PCR amplification of the plasmid to minimize thermal deamination of the nucleotide.
33. Urry, D. W. J. Protein Chem. 1988, 7, 1-34.
34. McMillan, R. A.; Caran, K.; Apkarian, R.; Conticello, V. P., manuscript in preparation.
35. Urry, D. W. Angew. Chem., Int. Ed. Engl. 1993, 32, 819-841.
36. Sambook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
37. The sequences of the oligonucleotides employed in the assembly of synthetic genes encoding 1 and 2 are given below with the region of 3′ complementarity in boldface. Sense strand for 1: 5′-AGTTAGGATCCCTCTTCAGTACCGGGT-GTTGGCGTTCCGGGTGTAGGTGTGCCAGGCAAGGG-TGTA- 3′. Antisense strand for 1: 5′-AGTTAGAATTCCTCT-TCATACGCCAACGCCTGGTACGCCAACACCCGG- TACACCCTTGCCTGGCACACC-3′. Sense strand for 2: 5′-AGTTAGGATCCCTCTTCAGTACCGGGTGTTGGC-GTTCCGGGTGTAGGTGTGCCAGGCATCGGTGTA- 3′. Antisense strand for 2: 5′-AGTTAGAATTCCTCTTCATACG-CCAACGCCTGGTACGCCAACACCCGGTACACC- GATGCCTGGCACACC-3′.
38. The sequences for the forward and reverse primers for the PCR amplification of pET-19b are given below with the regions of complementarity to the plasmid in boldface. Forward primer: 5′-AGTTACTCTTCAGTATAAGGATCCG-GCTGCTAACAAAG-3′. Reverse primer: 5′-AGTTACTCT-TCATACCATATGCTTGTCGTCGTCGTC-3′.
39. (a) Wasserman, Z. R.; Salemme, F. R. Biopolymers 1990, 29, 1613-1628.
40. (b) Chang, D. K.; Urry, D. W. Chem. Phys. Lett. 1988, 147, 395-400.
41. Bernard, P.; Gabant, P.; Bahassi, E. M.; Couturier, M. Gene 1994, 148, 71-74.
42. Colony screening PCR is the preferred method for the identification of multimer genes in the recombinant plasmids as it avoids parallel preparations of plasmid DNA from multiple colonies. We use a procedure recommended by Novagen, Inc., for amplification of the polylinker region using commercially available T7 promoter and terminator primers. The details of this procedure are described in their pET System Manual, which is available in hardcopy from Novagen, Inc., or in electronic HTML or PDF format at URL: (http:// www.novagen.com/html/techlit.html#pet).
43. Crowe, J.; Dobeli, H.; Gentz, R.; Hochuli, E.; Stuber, D.; Henco, K. Methods Mol. Biol. 1994, 31, 371-387.
44. Amino acid compositional analysis of the 90 kDa protein after cyanogen bromide cleavage is in excellent agreement with the expected composition of a polymer based on repeats of 1 and 2.