Poly(L-alanylglycine): Multigram-scale biosynthesis, crystallization, and structural analysis of chain-folded lamellae

Macromolecules

Volumn 30, Issue 1, 1997, Pages 42-49

  Panitch, Alyssa ^a   Matsuki, Kunio ^a   Cantor, Eric J ^a   Cooper, Sharon J ^b   Atkins, Edward D T ^b   Fournier, Maurille J ^a   Mason, Thomas L ^a   Tirrell, David A ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CHEMICAL ANALYSIS; CRYSTAL STRUCTURE; CRYSTALLIZATION; FERMENTATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS;

AMINO ACID ANALYSIS; CHAIN FOLDED LAMELLAE; ELEMENTAL ANALYSIS; ESCHERICHIA COLI; FED BATCH FERMENTATION; HIGH CELL DENSITY CULTURES; MULTIGRAM SCALE BIOSYNTHESIS; POLYALANYLGLYCINE; WIDE ANGLE X RAY DIFFRACTION;

ORGANIC POLYMERS;

EID: 0030785168     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma961059m     Document Type: Article

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34. 14b is not correct in detail. In addition, there are other discrepancies between the calculated and experimental X-ray diffraction patterns; there are unwanted Okl equatorial diffraction signals in the region between the 010 and 020, and there is poor agreement with the observed hoo intensities.
35. 4,5
36. The appearance of the first few orders of the 3.2 nm on the equator indicates that the lamellae are stacking reasonably well at the stacking periodicity of 3.2 nm. In a number of crystal preparations, only a diffuse first order at spacing 3.6 nm (12% greater) was observed. This is consistent with poorly stacked lamellae.
37. There are two possible sequences for the γ-turns, either GAG or AGA. Since we know a priori that the resolution of the experimental data will not allow us to delineate the fine details of the fold geometry, we chose the GAG γ-turn as the basis of our model.
38. With regard to the stacking of the polar apβ-sheets, there are two possibilities if like surfaces are to be in contact, either a rotation, π, about the a-axis, or a rotation, π, about the c-axis. The former structure was favored since it results in a chain-folded lamellar crystal with both surfaces equally populated with the two types of fold conformations. These considerations are discussed in more detail in ref 5.
39. 14a it may be that little, if any, chain folding occurred. No low-angle diffraction features were reported.
40. Cantor, E. J.; Atkins, E. D. T.; Cooper, S. J.; Fournier, M. J.; Mason, T. L.; Tirrell, D. A., submitted for publication.