Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP

Protein Expression and Purification

Volumn 7, Issue 1, 1996, Pages 51-57

  McPherson, David T ^a,b   Xu, Jie ^a,c   Urry, Dan W ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c Bioelastics Research Ltd   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

EID: 0030087535     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0008     Document Type: Article

References (26)

1. 1. Urry, D. W. (1993) Molecular Machines: How Motion and Other Functions of Living Organisms Can Result from Reversible Chemical Changes. Angew. Chem. (German) 105, 859-883; An-gew. Chem. Int. Ed. Engl. 32, 819-841.
2. 1. Urry, D. W. (1993) Molecular Machines: How Motion and Other Functions of Living Organisms Can Result from Reversible Chemical Changes. Angew. Chem. (German) 105, 859-883; An-gew. Chem. Int. Ed. Engl. 32, 819-841.
3. 2. Urry, D. W., Peng, S. Q., and Parker, T. (1993) Delineation of Electrostatic-and Hydrophobic-Induced pKa Shifts in Polypentapeptides: The Glutamic Acid Residue. J. Am. Chem. Soc. 115, 7509-7510.
4. 3. Urry, D. W., Peng, S. Q., Parker, T., Gowda, D. C., and Harris, R. D. (1993) Relative Significance of Electrostatic-and Hydrophobic-Induced pKa Shifts in a Model Protein: The Aspartic Acid Residue. Angew. Chem. (German) 105, 1523-1525; Angew. Chem. Int. Ed. Engl. 32, 1440-1442.
5. 3. Urry, D. W., Peng, S. Q., Parker, T., Gowda, D. C., and Harris, R. D. (1993) Relative Significance of Electrostatic-and Hydrophobic-Induced pKa Shifts in a Model Protein: The Aspartic Acid Residue. Angew. Chem. (German) 105, 1523-1525; Angew. Chem. Int. Ed. Engl. 32, 1440-1442.
6. 4. Urry, D. W., Peng, S. Q., Gowda, D. C., Parker, T., and Harris, R. D. (1994) Comparison of Electrostatic-and Hydrophobic-induced pKa Shifts in Polypentapeptides: The Lysine Residue. Chem. Phys. Lett. 225, 97-103.
7. 5. Urry, D. W. (1994) Postulates for Protein (Hydrophobic) Folding and Function. Int. J. Quant. Chem. Quant. Biol. Symp. 21, 3-15.
8. 6. Urry, D. W., Gowda, D. C., Peng, S. Q., and Parker, T. (1995) Non-linear Hydrophobic-induced pKa Shifts: Implications for Efficiency of Conversion to Chemical Energy. Chem. Phys. Lett. 239, 67-74.
9. 7. Urry, D. W., Gowda, D. C., Peng, S. Q., Parker, T., and Harris, R. (1992) Design at Nanometric Dimensions to Enhance Hydrophobicity-induced pKa Shifts. J. Am. Chem. Soc. 114, 8716-8717.
10. 8. Urry, D. W., Gowda, D. C., Peng, S. Q., Parker, T., Jing, N., and Harris, R. D. (1994) Nanometric Design of Extraordinary Hydrophobicity-induced pKa Shifts for Aspartic Acid: Relevance to Protein Mechanisms. Biopolymers 34, 889-896.
11. 9. Urry, D. W., (1995) Transductional Protein-based Polymers as New Controlled Release Vehicles, Part VI: New Biomaterials for Drug Delivery, in "Controlled Drug Delivery: The Next Generation" (Park, K., Ed.), Am. Chem. Soc. Professional Reference Book, in press Washington, DC.
12. 10. Urry, D. W., McPherson, D. T., Xu, J., Daniell, H., Guda, C., Gowda, D. C., Jing, N., and Parker, T. (1995) Protein-Based Polymeric Materials: Syntheses and Properties in "The Polymeric Materials Encyclopedia: Synthesis, Properties and Applications," in press CRC Press, Boca Raton, FL.
13. 11. Doel, M. T., Eaton, M., Cook, E. A., Lewis, H., Patel, T., and Carey, N. H. (1980) The expression in E. coli of synthetic repeating polymeric genes for poly(L-aspartyl-L-phenylalanine). Nucleic Acids Res. 8, 4575-4592.
14. 12. Salerno, A. J., and Goldberg, I. (1992) Expression of a synthetic mussel adhesive protein in E. coli. Proc. Am. Chem. Soc., Div. Polym. Mat. Sci. Eng. 66, 398.
15. 13. Beavis, R. C., Chait, B. T., Creel, H. S., Fournier, M. J., Mason, T. L., and Tirrell, D. A. (1992) Analysis of synthetic proteins by matrix assisted laser desorption mass spectrometry. Proc. Am. Chem. Soc., Div. Polym. Mat. Sci. Eng. 66, 27.
16. 14. Murata, T., Horinouchi, S., and Beppu, T. (1993) Production of poly(L-aspartyl-L-phenylalanine) in Escherichia coli. J. Biotechnol. 28, 301-312.
17. 15. Krejchi, M. T., Atkins, E. D. T., Waddon, A. J., Fournier, M. J., Mason, T. L., Tirrell, D. A. (1994) Chemical sequence control of β-sheet assembly in macromolecular crystals of periodic polypeptides. Science 265, 1427-1432.
18. 16. McPherson, D. T., Morrow, C., Minehan, D. S., Wu, J., Hunter, E., and Urry, D. W. (1992) Production and purification of a recombinant elastomeric polypeptide, G-(VPGVP)19-VPGV, from Escherichia coli. Biotechnol. Prog. 8, 347-352.
19. 17. Smith, D. B., and Johnson, K. S. (1988) Single-step purification of polypeptides expressed in E. coli as fusions with glutathione S-transferase. Gene 67, 31-40.
20. 18. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 polymerase to direct expression of cloned genes, in "Methods in Enzymology: Gene Expression Technology" (Goeddel, D. V., Ed.), Vol. 185, 60-89, Academic Press, San Diego.
21. 19. Urry, D. W. (1992) Free Energy Transduction in Polypeptides and Proteins Based on Inverse Temperature Transitions. Prog. Biophys. Molec. Biol. 57, 23-57.
22. 20. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1987) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
23. 21. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. 22. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
25. 23. Lee, C., Levin, A., and Branton, D. (1987) Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 166, 308-312.
26. 24. Urry, D. W. (1991) Thermally Driven Self-assembly, Molecular Structuring and Entropic Mechanisms in Elastomeric Polypeptides in "Molecular Conformation and Biological Interactions" (Balaram, P., and Ramaseshan, S., Eds.), pp. 555-583, Indian Acad. of Sci., Bangalore, India.