Targeting GFP to organelles

Methods in Cell Biology

Volumn , Issue 58, 1999, Pages 75-85

  De Giorgi, Francesca ^a   Ahmed, Zimran ^a   Bastianutto, Carlo ^a   Brini, Marisa ^a   Jouaville, Laurence Sophie ^a   Marsault, Robert ^a   Murgia, Marta ^a   Pinton, Paolo ^a   Pozzan, Tullio ^a   Rizzuto, Rosario ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN;

CELL CULTURE; CELL ORGANELLE; GENE EXPRESSION; GENETICS; MASS SPECTROMETRY; METABOLISM; REVIEW;

CELLS, CULTURED; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MASS SPECTROMETRY; ORGANELLES; RECOMBINANT FUSION PROTEINS;

EID: 0032622376     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (16)

1. Brewer, G. J., and Cotman, C. W. (1989). Survival and growth of hippocampal neurons in defined medium at low density: advantages of a sandwich culture technique or low oxygen. Brain Res. 494, 65-74.
2. 2+ concentration measured with specifically targeted recombinant aequorin. EMBO J. 12, 4813-4819.
3. 2+ homeostasis in primary cultures of skeletal myotubes. Mol. Cell. Biol. 8, 129-143.
4. Hartl, F. U., Pfanner, N., Nicholson, D. W., and Neupert, W. (1989). Mitochondrial protein import. Biochim. Biophys. Acta 988, 1-45.
5. Heim, R., Cubitt, A. B., and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
6. Hendrick, J. P., Hodges, P. E., and Rosenberg, L. E. (1989). Survey of amino-terminal proteolytic cleavage site in mitochondrial precursor proteins: leader peptide cleaved by two matrix proteases share a three-amino-acid motif. Proc. Natl. Acad. Sci. U. S. A. 86, 4056-4060.
7. 2+ beneath the plasma membrane of living A7r5 cells. EMBO J. 16, 1575-1581.
8. Masibay, A. S., Balaji, P. V., Boeggeman, E. E., and Qasba, P. K. (1993). Mutational analysis of the Golgi retention signal of bovine. J. Biol. Chem. 268, 9908-9916.
9. 2+ concentration in the endoplasmic reticulum of intact cells. EMBO J. 14, 5467-5475.
10. Munro, S., and Pelham, H. R. B. (1987). A C-terminal signal prevents secretion of luminal ER proteins. Cell 48, 899-907.
11. Picard, D., and Yamamoto, K. R. (1987). Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO J. 6, 3333-3340.
12. 2+ revealed by specifically targeted recombinant aequorin. Nature 358, 325-328.
13. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M., and Pozzan, T. (1995). Chimeric green fluorescent protein: a new tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635-642.
14. Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R. Y., and Pozzan, T. (1996). Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6, 183-188.
15. Schwientek, T., Lorenz, C., and Ernst, J. F. (1995). Golgi localization in yeast is mediated by the membrane anchor region of rat liver sialyltransferase. J. Biol. Chem. 270, 5483-5489.
16. Sitia, R., and Meldolesi, J. (1992). Endoplasmic reticulum: a dynamic patchwork of specialized subregions. Mol. Biol. Cell 3, 1067-1072.