The role of CCR7 in T(H)1 and T(H)2 cell localization and delivery of B cell help in vivo

Science

Volumn 286, Issue 5447, 1999, Pages 2159-2162

  Randolph, David A ^a,b   Huang, Guangming ^c   Carruthers, Cynthia J L ^c   Bromley, Lindsay E ^c   Chaplin, David D ^a,b,c  

^a Division of Allergy and Immunology   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CHEMOKINE; CHEMOKINE RECEPTOR; CALCIUM; CC CHEMOKINE RECEPTOR 7; OVALBUMIN;

ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; CELL MIGRATION; CELLULAR DISTRIBUTION; HELPER CELL; IMMUNE RESPONSE; LYMPH FOLLICLE; LYMPHOID TISSUE; PRIORITY JOURNAL; SPLEEN; T LYMPHOCYTE SUBPOPULATION; ADOPTIVE TRANSFER; ANIMAL; BAGG ALBINO MOUSE; CELL MOTION; GENETIC TRANSFECTION; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; MOUSE; SIGNAL TRANSDUCTION; TH1 CELL; TH2 CELL; TRANSGENIC MOUSE;

ADOPTIVE TRANSFER; ANIMALS; B-LYMPHOCYTES; CALCIUM; CELL MOVEMENT; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; MICE, TRANSGENIC; OVALBUMIN; RECEPTORS, CHEMOKINE; SIGNAL TRANSDUCTION; SPLEEN; TH1 CELLS; TH2 CELLS; TRANSFECTION;

EID: 0032589575     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5447.2159     Document Type: Article

References (45)

1. A. J. van den Eertwegh, J. D. Laman, R. J. Noelle, W. J. Boersma, E. Claassen, Semin. Immunol. 6, 327 (1994); I. C. MacLennan et al., Immunol. Rev. 156, 53 (1997); Y. J. Liu, J. Zhang, P. J. Lane, E. Y. Chan, I. C. MacLennan, Eur. J. Immunol. 21, 2951 (1991); J. Jacob, R. Kassir, G. Kelsoe, J. Exp. Med. 173, 1165 (1991).
2. A. J. van den Eertwegh, J. D. Laman, R. J. Noelle, W. J. Boersma, E. Claassen, Semin. Immunol. 6, 327 (1994); I. C. MacLennan et al., Immunol. Rev. 156, 53 (1997); Y. J. Liu, J. Zhang, P. J. Lane, E. Y. Chan, I. C. MacLennan, Eur. J. Immunol. 21, 2951 (1991); J. Jacob, R. Kassir, G. Kelsoe, J. Exp. Med. 173, 1165 (1991).
3. A. J. van den Eertwegh, J. D. Laman, R. J. Noelle, W. J. Boersma, E. Claassen, Semin. Immunol. 6, 327 (1994); I. C. MacLennan et al., Immunol. Rev. 156, 53 (1997); Y. J. Liu, J. Zhang, P. J. Lane, E. Y. Chan, I. C. MacLennan, Eur. J. Immunol. 21, 2951 (1991); J. Jacob, R. Kassir, G. Kelsoe, J. Exp. Med. 173, 1165 (1991).
4. A. J. van den Eertwegh, J. D. Laman, R. J. Noelle, W. J. Boersma, E. Claassen, Semin. Immunol. 6, 327 (1994); I. C. MacLennan et al., Immunol. Rev. 156, 53 (1997); Y. J. Liu, J. Zhang, P. J. Lane, E. Y. Chan, I. C. MacLennan, Eur. J. Immunol. 21, 2951 (1991); J. Jacob, R. Kassir, G. Kelsoe, J. Exp. Med. 173, 1165 (1991).
5. P. Garside et al., Science 281, 96 (1998).
6. J. G. Cyster, J. Exp. Med. 189, 447 (1999).
7. G. J. Spangrude, F. Sacchi, H. R. Hill, D. E. Van Epps, R. A. Daynes, J. Immunol. 135, 4135 (1985); J. G. Cyster and C. C. Goodnow, J. Exp. Med. 182, 581 (1995).
8. G. J. Spangrude, F. Sacchi, H. R. Hill, D. E. Van Epps, R. A. Daynes, J. Immunol. 135, 4135 (1985); J. G. Cyster and C. C. Goodnow, J. Exp. Med. 182, 581 (1995).
9. R. Yoshida et al., J. Biol. Chem. 272, 13803 (1997); M. D. Gunn et al., Proc. Natl. Acad. Sci. U.S.A. 95, 258 (1998); M. D. Gunn et al., Nature 391, 799 (1998); K. Willimann et al., Eur. J. Immunol. 28, 2025 (1998); V. N. Ngo, H. L. Tang, J. G. Cyster, J. Exp. Med. 188, 181 (1998).
10. R. Yoshida et al., J. Biol. Chem. 272, 13803 (1997); M. D. Gunn et al., Proc. Natl. Acad. Sci. U.S.A. 95, 258 (1998); M. D. Gunn et al., Nature 391, 799 (1998); K. Willimann et al., Eur. J. Immunol. 28, 2025 (1998); V. N. Ngo, H. L. Tang, J. G. Cyster, J. Exp. Med. 188, 181 (1998).
11. R. Yoshida et al., J. Biol. Chem. 272, 13803 (1997); M. D. Gunn et al., Proc. Natl. Acad. Sci. U.S.A. 95, 258 (1998); M. D. Gunn et al., Nature 391, 799 (1998); K. Willimann et al., Eur. J. Immunol. 28, 2025 (1998); V. N. Ngo, H. L. Tang, J. G. Cyster, J. Exp. Med. 188, 181 (1998).
12. R. Yoshida et al., J. Biol. Chem. 272, 13803 (1997); M. D. Gunn et al., Proc. Natl. Acad. Sci. U.S.A. 95, 258 (1998); M. D. Gunn et al., Nature 391, 799 (1998); K. Willimann et al., Eur. J. Immunol. 28, 2025 (1998); V. N. Ngo, H. L. Tang, J. G. Cyster, J. Exp. Med. 188, 181 (1998).
13. R. Yoshida et al., J. Biol. Chem. 272, 13803 (1997); M. D. Gunn et al., Proc. Natl. Acad. Sci. U.S.A. 95, 258 (1998); M. D. Gunn et al., Nature 391, 799 (1998); K. Willimann et al., Eur. J. Immunol. 28, 2025 (1998); V. N. Ngo, H. L. Tang, J. G. Cyster, J. Exp. Med. 188, 181 (1998).
14. R. Forster et al., Cell 87, 1037 (1996).
15. R. Forster et al., Cell 99, 23 (1999).
16. V. N. Ngo et al., J. Exp. Med. 189, 403 (1999); M. D. Gunn et al., J. Exp. Med. 189, 451 (1999).
17. V. N. Ngo et al., J. Exp. Med. 189, 403 (1999); M. D. Gunn et al., J. Exp. Med. 189, 451 (1999).
18. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
19. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
20. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
21. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
22. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
23. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
24. T. R. Mosmann, H. Cherwinski, M. W. Bond, M. A. Giedlin, R. L. Coffman, J. Immunol. 136, 2348 (1986); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996); A. K. Abbas, S. Urioste, T. L. Collins, W. H. Boom, J. Immunol. 144, 2031 (1990); W. H. Boom, D. Liano, A. K. Abbas, J. Exp. Med. 167, 1350 (1988); A. K. Abbas, H. J. Burstein, S. A. Bogen, Semin. Immunol. 5, 441 (1993); G. F. Del Prete, M. De Carli, M. Ricci, S. Romagnani, J. Exp. Med. 174, 809 (1991); L. Killar, G. MacDonald, J. West, A. Woods, K. Bottomly, J. Immunol. 138, 1674 (1987).
25. T. L. Stevens et al., Nature 334, 255 (1988).
26. F. Sallusto, A. Lanzavecchia, C. R. Mackay, Immunol. Today 19, 568 (1998); S. G. Ward, K. Bacon, J. Westwick, Immunity 9, 1 (1998); F. Sallusto, C. R. Mackay, A. Lanzavecchia, Science 277, 2005 (1997); R. Bonecchi et al., J. Exp. Med. 187, 129 (1998); F. Sallusto, D. Lenig, C. R. Mackay, A. Lanzavecchia, J. Exp. Med. 187, 875 (1998).
27. F. Sallusto, A. Lanzavecchia, C. R. Mackay, Immunol. Today 19, 568 (1998); S. G. Ward, K. Bacon, J. Westwick, Immunity 9, 1 (1998); F. Sallusto, C. R. Mackay, A. Lanzavecchia, Science 277, 2005 (1997); R. Bonecchi et al., J. Exp. Med. 187, 129 (1998); F. Sallusto, D. Lenig, C. R. Mackay, A. Lanzavecchia, J. Exp. Med. 187, 875 (1998).
28. F. Sallusto, A. Lanzavecchia, C. R. Mackay, Immunol. Today 19, 568 (1998); S. G. Ward, K. Bacon, J. Westwick, Immunity 9, 1 (1998); F. Sallusto, C. R. Mackay, A. Lanzavecchia, Science 277, 2005 (1997); R. Bonecchi et al., J. Exp. Med. 187, 129 (1998); F. Sallusto, D. Lenig, C. R. Mackay, A. Lanzavecchia, J. Exp. Med. 187, 875 (1998).
29. F. Sallusto, A. Lanzavecchia, C. R. Mackay, Immunol. Today 19, 568 (1998); S. G. Ward, K. Bacon, J. Westwick, Immunity 9, 1 (1998); F. Sallusto, C. R. Mackay, A. Lanzavecchia, Science 277, 2005 (1997); R. Bonecchi et al., J. Exp. Med. 187, 129 (1998); F. Sallusto, D. Lenig, C. R. Mackay, A. Lanzavecchia, J. Exp. Med. 187, 875 (1998).
30. F. Sallusto, A. Lanzavecchia, C. R. Mackay, Immunol. Today 19, 568 (1998); S. G. Ward, K. Bacon, J. Westwick, Immunity 9, 1 (1998); F. Sallusto, C. R. Mackay, A. Lanzavecchia, Science 277, 2005 (1997); R. Bonecchi et al., J. Exp. Med. 187, 129 (1998); F. Sallusto, D. Lenig, C. R. Mackay, A. Lanzavecchia, J. Exp. Med. 187, 875 (1998).
31. 7 T cells were washed, resuspended in 0.25 ml of phosphate-buffered saline (PBS) and then injected intravenously (iv) into mice that had been anesthetized with Metofane. As indicated, some mice were also injected with OVA (100 μg) in 50 μl of incomplete Freund's adjuvant administered intraperitoneally or subcutaneously in the footpad. In some experiments, T cells were incubated with pertussis toxin (100 ng/ml) for 2 hours at 37°C and then washed five times before transfer.
32. 2 in methanol to quench endogenous peroxidase activity. Color development was for 1 hour with the AP-ABC reagent (Vector Labs) together with horseradish peroxidase - anti-FITC (Sigma). Alkaline phosphatase activity was detected with the Vector Blue Substrate Kit (Vector), and peroxidase activity was detected with Sigma Fast diaminobenzidine substrate (Sigma).
33. H2 cells 7 days after initiation of culture under polarizing conditions with guanidine isothiocyanate-CsCl [J. M. Chirgwin, A. E. Przybyla, R. J. MacDonald, W. J. Rutter, Biochemistry 18, 5294 (1979)]. Ribonuclease protection assays were performed with 10 μg of RNA (RiboQuant kit, Pharmingen) with the mCR-5 and mCR-6 template sets. For Northern (RNA) blot analysis, 40-μg samples of RNA were fractionated with 1% agarose gel in formaldehyde and transferred to a nylon membrane (Hybond-N, Amersham Life Science). Gene expression was detected with radiolabeled probes (Multiprime system, Amersham Life Science). For CXCR3, the entire coding region was used as a probe. For CCR7, a 415-base pair Nco I-Hind III fragment was used. Hybridization was at 42°C in 50% formamide and washing was in 0.2× standard saline citrate at 42°C.
34. D. Randolph and D. Chaplin, unpublished data.
35. 2+ concentration had returned to a stable baseline, 10 μM ionomycin, 0.1% Triton-X 100, and 4 mM EGTA were added sequentially as controls.
36. -l mice were activated under polarizing conditions with OVA (323-339) peptide and infected 24 hours later with retroviral supernatants and polybrene (6 μg/ml, Sigma). Six days after activation, transduced cells were purified with FITC - anti-huCD4 followed by anti-FITC MicroBeads on a MiniMACS column (Miltenyi Biotec, Auburn, CA). Purified huCD4-expressing cells were restimulated with OVA peptide and irradiated BALB/c splenocytes and either used directly 7 days later or frozen for subsequent use.
37. Mig has previously been reported to be preferred over SLC as a ligand for CXCR3 [H. Soto et al., Proc. Natl. Acad. Sci. U.S.A. 95, 8205 (1998)].
38. D. Randolph, G. Huang, D. Chaplin, unpublished data.
39. H2 cells in T cell medium containing either NP-OVA (10 μg/ml) or NP - keyhole limpet hemocyanin (10 μ/ml). After 2 days, the medium containing the antigen was removed and replaced with fresh medium. Five days later, culture supematants were collected and anti-NP IgG1 titers were measured.
40. D. Randolph, G. Huang, D. Chaplin, unpublished data.
41. K. U. Willfuhr, J. Westermann, R. Pabst, Eur. J. Immunol. 20, 903 (1990); R. Pabst and J. Westermann, Scanning Microsc. 5, 1075 (1991).
42. K. U. Willfuhr, J. Westermann, R. Pabst, Eur. J. Immunol. 20, 903 (1990); R. Pabst and J. Westermann, Scanning Microsc. 5, 1075 (1991).
43. B. Pulendran et al., J. Immunol. 159, 2222 (1997).
44. B. Pulendran et al., Proc. Natl. Acad. Sci. U.S.A. 96, 1036 (1999).
45. -l- mouse strain. Supported by NIH grants T32 GM07200 (to D.A.R.) and AI34580 (to D.D.C.). D.D.C. is an investigator of the Howard Hughes Medical Institute.