Myr+-G(i2)α and G(o)α subunits restore the efficacy of opioids, clonidine and neurotensin giving rise to antinociception in G-protein knock-down mice

Neuropharmacology

Volumn 38, Issue 12, 1999, Pages 1861-1873

  Garzón, Javier ^a   Rodríguez Díaz, Marta ^a   Deantonio, Isabel ^a   Defelipe, Javier ^a   Rodríguez, José Rodrigo ^a   Sánchez Blázquez, Pilar ^a  

^a CSIC   (Spain)

Author keywords

Antinociception; Clonidine; G subunits; G proteins; Neurotensin; Opioids

Indexed keywords

1 (5 ISOQUINOLINESULFONYL) 2 METHYLPIPERAZINE; ANALGESIC AGENT; ANTISENSE OLIGODEOXYNUCLEOTIDE; BETA ENDORPHIN; CLONIDINE; ENKEPHALIN[2,5 DEXTRO PENICILLAMINE]; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (2 THIODIPHOSPHATE); GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE TRIPHOSPHATE; MORPHINE; N (2 METHYLAMINOETHYL) 5 ISOQUINOLINESULFONAMIDE; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; NEUROTENSIN; PERTUSSIS TOXIN; PHENYLISOPROPYLADENOSINE; PROTEIN KINASE C; PROTEIN SUBUNIT; BORDETELLA VIRULENCE FACTOR; GUANOSINE TRIPHOSPHATASE; NARCOTIC AGENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTINOCICEPTION; ARTICLE; ASTROCYTE; BRAIN NERVE CELL; CELL NUCLEUS; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPLASM; DRUG DISTRIBUTION; DRUG UPTAKE; ELECTRON MICROSCOPY; FLUORESCENCE; HYDROLYSIS; INTERNALIZATION; INTRACEREBROVENTRICULAR DRUG ADMINISTRATION; INTRAVAGINAL DRUG ADMINISTRATION; KNOCKOUT MOUSE; MALE; MOUSE; MYRISTYLATION; NERVE CELL CULTURE; NERVE CELL MEMBRANE; NEUROLOGIC DISEASE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT; SIGNAL TRANSDUCTION; SYNAPSE; ANIMAL; BRAIN CORTEX; CELL CULTURE; DRUG EFFECT; EMBRYO; GENETICS; METABOLISM; PHYSIOLOGY;

ANALGESICS; ANIMALS; CELLS, CULTURED; CEREBRAL CORTEX; CLONIDINE; EMBRYO; ENKEPHALIN, D-PENICILLAMINE (2,5)-; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; INJECTIONS, INTRAVENTRICULAR; MALE; MICE; NARCOTICS; NEUROTENSIN; PERTUSSIS TOXIN; VIRULENCE FACTORS, BORDETELLA;

EID: 0032585754     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(99)00070-2     Document Type: Article

References (47)

1. Aridor M., Rajmilevich G., Beaven M.A., Sagi-Eisenberg R. Activation of exocytosis by the heterotrimeric G protein Gi3. Science. 262:1993;1569-1572.
2. Brightman M.W. The intracerebral movement of proteins injected into blood and cerebrospinal fluid of mice. Prog. Brain Res. 29:1968;19-37.
3. iα to the nucleus. FASEB J. 5:1991;200-206.
4. Crouch M.F., Heydon K., Garnaut S.M., Milburn P.J., Hendry I.A. Retrograde axonal transport of the alpha-subunit of the GTP-binding protein Gz in mouse sciatic nerve: a potential pathway for signal transduction in neurons. Eur. J. Neurosci. 6:1994;626-631.
5. DeFelipe J., Marco P., Sola R.G., Sánchez-Blázquez P., Garzón J. Local changes in GTP-binding protein immunoreactivities in human epileptogenic neocortex. Exp. Brain Res. 119:1998;153-158.
6. Dichter M.A. Rat cortical neurons in cell culture: culture methods, cell morphology, electrophysiology, and synapse formation. Brain Res. 149:1978;279-293.
7. Gallego C., Gupta S.K., Winitz S., Eisfelder B.J., Johnson G.L. Myristoylation of the G alpha i2 polypeptide, a G protein alpha subunit, is required for its signalling and transformation functions. Proc. Natl. Acad. Sci. USA. 89:1992;9695-9699.
8. i2 transducer proteins. Life Sci/Pharmacol. Lett. 55:1994;PL205-212.
9. Garzón J., García-España A., Sánchez-Blázquez P. Opioids binding mu and delta receptors exhibit diverse efficacy in the activation of Gi2 and Gx/z transducer proteins in mouse periaqueductal gray matter. J. Pharmacol. Exp. Ther. 281:1997;549-557.
10. m GTPase activity in mouse periaqueductal grey matter. Eur. J. Neurosci. 9:1997;1194-1200.
11. Garzón J., Castro M., Sánchez-Blázquez P. Influence of Gz and Gi2 transducer proteins in the affinity of opioid agonists to μ receptors. Neuroscience. 10:1998;2557-2564.
12. Garzón J., Sánchez-Blázquez P. Microinjection of G protein α-subunits into the lateral ventricle of the mouse is followed by internalization and functional coupling to receptors. XIIIth Inter. Cong. Pharmacol. (IUPHAR). Naunyn-Schmied. Arch. Pharmacol. 358(Suppl. 2):1998;R648.
13. Graber S.G., Figler R.A., Garrison J.C. Expression and purification of functional G protein α subunits using a baculovirus expression system. J. Biol. Chem. 267:1992;1271-1278.
14. Helms J.B. Role of heterotrimeric GTP binding proteins in vesicular protein transport: indications for both classical and alternative G protein cycles. FEBS Lett. 369:1995;84-88.
15. Hendry I.A., Crouch M.F. Retrograde axonal transport of the GTP-binding protein Gi alpha: a potential neurotrophic intra-axonal messenger. Neurosci. Lett. 133:1991;29-32.
16. Jimi T., Wakayama Y., Shibuya S., Nakata H., Tomaru T., Takahashi Y., Kosada K., Asano T., Kato K. High levels of nervous system-specific proteins in cerebrospinal fluid in patients with early stage Creutzfeldt-Jakob disease. Clin. Chim. Acta. 211:1992;37-46.
17. Jones, T.L.Z., Simonds, W.F., Meredino, J.J., Jr., Brann, M.R., Spiegel, A.M., 1990. Myristoylation of an inhibitory GTP-binding protein α subunit is essential for its membrane attachment. Proc. Natl. Acad. Sci. USA 87, 568-572.
18. Kelly J.F., Furukawa K., Barger S.W., Renger M.R., Mark R.J., Blanc E.M., Roth G.S., Mattson M.P. Amyloid-β-peptide disrupts carbachol-induced muscarinic cholinergic signal transduction in cortical neurons. Proc. Natl. Acad. Sci. USA. 93:1996;6753-6758.
19. Kenakin T. Agonist-receptor efficacy II: agonist trafficking of receptor signals. Trends Pharmacol. Sci. 16:1995;232-238.
20. Knopf J.L., Lee M-H, Sultzman L.A., Kriz R.W., Loomis C.R., Hewick R.M., Bell R.M. Cloning and expression of multiple protein kinase C cDNAs. Cell. 46:1986;491-502.
21. Lambright D.G., Noel J.P., Hamm H.E., Sigler P.B. Structural determinants for activation of the α-subunits of a heterotrimeric G protein. Nature. 369:1994;621-628.
22. Law S.F., Zaina S., Sweet R., Yasuda K., Bell G.I., Stadel J., Reisine T. Giα1 selectively couples somatostatin receptor subtype 3 to adenylyl cyclase: identification of the functional domains of this α subunit necessary for mediating the inhibition by somatostatin of cAMP formation. Mol. Pharmacol. 45:1994;587-590.
23. Leyte A., Barr B.A., Kehlenbach R.H., Huttner W.B. Multiple trimeric G-proteins on the trans-Golgi network exert stimulatory and inhibitory effects on secretory vesicle formation. EMBO J. 11:1992;4795-4804.
24. Li X., Greenwood A.F., Powers R., Jope R.S. Effects of postmortem interval, age, and Alzheimer's disease on G-proteins in human brain. Neurobiol. Aging. 17:1996;115-122.
25. i2 in GH4Cl pituitary cells. J. Biol. Chem. 269:1994;13880-13886.
26. Manji K.H., Chen G., Shimon H., Hsiao J.K., Potter W.Z., Belmaker R.H. Guanine nucleotide-binding proteins in bipolar affective disorder. Effects of long-term lithium treatment. Arch. Gen. Psych. 52:1995;135-144.
27. o, from the plasma membrane of NG108-15 cells. Biochem. Biophys. Res. Comm. 152:1988;243-251.
28. McCarthy K.D., deVellis J. Preparation of separated astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85:1980;890-902.
29. Murayama T., Takahashi A., Asano T., Kato K. Elevated levels of the α subunit of GTP-binding protein Go in cerebrospinal fluid of patients with neurological disorders. J. Mol. Neurosci. 1:1989;27-32.
30. oα immunoreactivities in left temporal cortices from patients with schizophrenia. Brain Res. 615:1993;41-49.
31. Offermanns S., Wieland T., Homann D., Sandmann J., Bombien E., Spicher K., Schultz G., Jakobs K.H. Transfected muscarinic acetylcholine receptors selectively couple to Gi-type G proteins and Gq/11. Mol. Pharmacol. 45:1994;890-898.
32. Pastan I., Willingham M., Amderson W., Gallo M. Localization of serum-derived alpha 2 macroglobulin in cultured cells and decrease after Moloney sarcoma virus transformation. Cell. 12:1977;609-617.
33. Péraldi, S., Nguyen Than Dao, B., Brabet, Ph., Homburger, V., Rouot, B., Toutant, M., Bouille, Ch., Assenmacher, I., Bockaert, J. Gabrion, J., 1989. Apical localization of the alpha subunit of GTP-binding protein Go in choroidal and ciliated ependymocytes. J. Neurosci. 9, 806-814.
34. Robb S., Cheek T.R., Hannan F.L., Hall L.M., Midgley J.M., Evans P.D. Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems. EMBO J. 13:1994;1325-1330.
35. Sánchez-Blázquez P., Garzón J. Pertussis toxin differentially reduces the efficacy of opioids to produce supraspinal analgesia in the mouse. Eur. J. Pharmacol. 152:1988;357-361.
36. i2 transducer proteins on μ/δ opioid mediated supraspinal antinociception. Life Sci. Pharmacol. Lett. 53:1993;PL381-386.
37. Sánchez-Blázquez P., García-España A., Garzón J. In vivo injection of oligodeoxynucleotides to Gα subunits and supraspinal analgesia evoked by mu and delta opioid agonists. J. Pharmacol. Exp. Ther. 275:1995;1590-1596.
38. Sánchez-Blázquez P., García-España A., Garzón J. Antisense oligodeoxynucleotides to opioid Mu and DELTA receptors reduced morphine dependence in mice: role of DELTA2 opioid receptors. J. Pharmacol. Exp. Ther. 280:1997;1423-1431.
39. 35S]GTPγS autoradiography in rat brain. J. Neurosci. 16:1996;2684-2692.
40. Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P.H., Journot L. Differential signal transduction by five splice variants of the PACAP receptor. Nature. 365:1993;170-175.
41. Standifer K.M., Rossi G.C., Pasternak G.W. Differential blockade of opioid analgesia by antisense oligodeoxynucleotides directed against various G protein α subunits. Mol. Pharmacol. 50:1996;293-298.
42. Stein C.A., Tonkinson J.L., Zhang L.M., Yakublv L., Gervasoni J., Taub R., Rotenberg S.A. Dynamics of the internalization of phosphodiester oligodeoxynucleotides in HL60, cells. Biochemistry. 32:1993;4855-4861.
43. Ueda H., Yoshihara Y., Misawa H., Fukushima N., Ui M., Takagi H., Satoh M. The kyotorphin (tyrosine-arginine) receptor and a selective reconstitution with purified Gi, measured with GTPase and phospholipase C assays. J. Biol. Chem. 264:1989;3732-3741.
44. van Renswoude J., Bridges K.R., Harford J., Klausner R.D. Receptor-mediated endocytosis of transferrin and the uptake of Fe in K562 cells: identification of a nonlysosomal acidic compartment. Proc. Natl. Acad. Sci. USA. 79:1982;6186-6190.
45. Yakubov L.A., Deeva E.A., Zarytova V.F., Ivanova E.M., Ryte A.S., Yurchenko L.V., Vlassov V.V. Mechanism of oligonucleotide uptake by cells: involvement of specific receptors? Proc. Natl. Acad. Sci. USA. 86:1989;6454-6458.
46. Willingham M.C., Hanover J.A., Dickson R.B., Pastan I. Morphologic characterization of the pathway of transferrin endocytosis and recycling in human KB cells. Proc. Natl. Acad. Sci. USA. 80:1984;175-179.
47. 2+- and temperature-sensitive GTP-binding protein. Biochem. J. 232:1985;191-197.