Functional reconstitution of Ypt7p GTPase and a purified vacuole SNARE complex

Science

Volumn 281, Issue 5377, 1998, Pages 700-702

  Sato, Ken ^a   Wickner, William ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; MEMBRANE PROTEIN;

ARTICLE; CELL ORGANELLE; CELL VACUOLE; MEMBRANE FUSION; MEMBRANE RECONSTITUTION; NONHUMAN; PRIORITY JOURNAL; PROTEOLIPOSOME; SACCHAROMYCES CEREVISIAE;

EID: 0032584707     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5377.700     Document Type: Article

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22. Saccharomyces cerevisiae strains used (6) were BJ3505 (pep4::HIS3), DKY6281 (pho8::TRP1), and K91-1A (pho8::pAL134 pho13::pH13). Vacuole and cytosol preparation and the standard fusion reaction were as described (5, 6).
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32. We thank N. Margolis for expert assistance and R. Schekman, C. Barlowe, Z. Xu, C. Ungermann, G. Eitzen, J. Eichler, F. Duong, and A. Price for discussions. Supported by the National Institute of General Medical Sciences. K.S. is supported by a Japan Society for the Promotion of Science Fellowship and an Human Frontier Science Program Long-Term Fellowship.