-
3
-
-
0027517462
-
-
T. Söllner, M. K. Bennett, S. W. Whiteheart, R. H. Scheller, J. E. Rothman, Cell 75, 409 (1993).
-
(1993)
Cell
, vol.75
, pp. 409
-
-
Söllner, T.1
Bennett, M.K.2
Whiteheart, S.W.3
Scheller, R.H.4
Rothman, J.E.5
-
5
-
-
0027328483
-
-
M. K. Bennett et al., Cell 74, 863 (1993).
-
(1993)
Cell
, vol.74
, pp. 863
-
-
Bennett, M.K.1
-
6
-
-
0027997974
-
-
D. K. Banfield, M. J. Lewis, C. Rabouille, G. Warren, H. R. B. Pelham, J. Cell Biol. 127, 357 (1994).
-
(1994)
J. Cell Biol.
, vol.127
, pp. 357
-
-
Banfield, D.K.1
Lewis, M.J.2
Rabouille, C.3
Warren, G.4
Pelham, H.R.B.5
-
10
-
-
0028971172
-
-
M. Aridor, S. I. Bannykh, T. Rowe, W. E. Balch, ibid. 131, 875 (1995).
-
(1995)
J. Cell Biol.
, vol.131
, pp. 875
-
-
Aridor, M.1
Bannykh, S.I.2
Rowe, T.3
Balch, W.E.4
-
11
-
-
0001353452
-
-
B. L. Tang, S. H. Low, H.-P. Hauri, W. Hong, Eur. J. Cell Biol. 68, 397 (1995).
-
(1995)
Eur. J. Cell Biol.
, vol.68
, pp. 397
-
-
Tang, B.L.1
Low, S.H.2
Hauri, H.-P.3
Hong, W.4
-
14
-
-
0029836930
-
-
T. Rowe, M. Aridor, J. M. McCaffery, H. Plutner, W. E. Balch, J. Cell Biol. 135, 895 (1996).
-
(1996)
J. Cell Biol.
, vol.135
, pp. 895
-
-
Rowe, T.1
Aridor, M.2
McCaffery, J.M.3
Plutner, H.4
Balch, W.E.5
-
20
-
-
0030890594
-
-
J. C. Hay, D. S. Chao, C. S. Kuo, R. H. Scheller, Cell 89, 149 (1997).
-
(1997)
Cell
, vol.89
, pp. 149
-
-
Hay, J.C.1
Chao, D.S.2
Kuo, C.S.3
Scheller, R.H.4
-
22
-
-
0027413655
-
-
T. Söllner et al., Nature 362, 318 (1993).
-
(1993)
Nature
, vol.362
, pp. 318
-
-
Söllner, T.1
-
27
-
-
0028574245
-
-
J. P. Lian, S. Stone, Y. Jiang, P. Lyons, S. Ferro-Novick, Nature 372, 698 (1994).
-
(1994)
Nature
, vol.372
, pp. 698
-
-
Lian, J.P.1
Stone, S.2
Jiang, Y.3
Lyons, P.4
Ferro-Novick, S.5
-
28
-
-
0028168008
-
-
M. Sögaard et al., Cell 78, 937 (1994).
-
(1994)
Cell
, vol.78
, pp. 937
-
-
Sögaard, M.1
-
29
-
-
23444432144
-
-
W. E. Balch, J. M. McCaffery, H. Plutner, M. G. Farquhar, ibid. 76, 841 (1994).
-
(1994)
Cell
, vol.76
, pp. 841
-
-
Balch, W.E.1
McCaffery, J.M.2
Plutner, H.3
Farquhar, M.G.4
-
30
-
-
0026439921
-
-
H. Plutner, H. W. Davidson, J. Saraste, W. E. Balch, J. Cell Biol. 119, 1097 (1992).
-
(1992)
J. Cell Biol.
, vol.119
, pp. 1097
-
-
Plutner, H.1
Davidson, H.W.2
Saraste, J.3
Balch, W.E.4
-
31
-
-
0030968878
-
-
B. J. Nichols, C. Ungermann, H. R. B. Pelham, T. Wickner, A. Haas, Nature 387, 199 (1997).
-
(1997)
Nature
, vol.387
, pp. 199
-
-
Nichols, B.J.1
Ungermann, C.2
Pelham, H.R.B.3
Wickner, T.4
Haas, A.5
-
32
-
-
0028978947
-
-
L. H. Chamberlain, D. Roth, A. Morgan, R. D. Burgoyne, J. Cell Biol. 130, 1063 (1995).
-
(1995)
J. Cell Biol.
, vol.130
, pp. 1063
-
-
Chamberlain, L.H.1
Roth, D.2
Morgan, A.3
Burgoyne, R.D.4
-
33
-
-
0029808089
-
-
A. Banerjee, V. A. Barry, B. R. DasGupta, T. F. J. Martin, J. Biol. Chem. 271, 20223 (1996).
-
(1996)
J. Biol. Chem.
, vol.271
, pp. 20223
-
-
Banerjee, A.1
Barry, V.A.2
Dasgupta, B.R.3
Martin, T.F.J.4
-
34
-
-
0030740252
-
-
P. I. Hanson, R. Roth, H. Morisaki, R. Jahn, J. E. Heuser, Cell 90, 523 (1997).
-
(1997)
Cell
, vol.90
, pp. 523
-
-
Hanson, P.I.1
Roth, R.2
Morisaki, H.3
Jahn, R.4
Heuser, J.E.5
-
35
-
-
0028233498
-
-
C. Barlowe et al., ibid. 77, 895 (1994).
-
(1994)
Cell
, vol.77
, pp. 895
-
-
Barlowe, C.1
-
36
-
-
0030955328
-
-
J. F. Presley et al., Nature 389, 81 (1997).
-
(1997)
Nature
, vol.389
, pp. 81
-
-
Presley, J.F.1
-
40
-
-
0026533579
-
-
D. W. Wilson, S. W. Whiteheart, M. Wiedmann, M. Brunner, J. E. Rothman, ibid. 117, 531 (1992).
-
(1992)
J. Cell Biol.
, vol.117
, pp. 531
-
-
Wilson, D.W.1
Whiteheart, S.W.2
Wiedmann, M.3
Brunner, M.4
Rothman, J.E.5
-
42
-
-
0024340753
-
-
V. Malhotra, T. Serafini, L Orci, J. C. Shepherd, J. E. Rothman, Cell 58, 329 (1989).
-
(1989)
Cell
, vol.58
, pp. 329
-
-
Malhotra, V.1
Serafini, T.2
Orci, L.3
Shepherd, J.C.4
Rothman, J.E.5
-
43
-
-
6844221854
-
-
note
-
NRK cells on cover slips were infected at the restrictive temperature (39.5°C) with the tsO45 strain of VSV (30). The cells were subsequently shifted to either 15° or 32°C, then permeabilized with digitonin and probed with the appropriate primary antibodies followed by fluorescently labeled secondary antibodies (30). The primary antibodies used were directed against Syn5 (17), VSV-G (p5D4) (38), and α-1,2-mannosidase II (39).
-
-
-
-
44
-
-
6844227661
-
-
note
-
NRK cells were infected with tsO45 VSV for 4 hours at 39.5°C, then transferred to ice and permeabilized (18). Transport reactions containing semi-intact cells, gel-filtered cytosol, ATP mix, and uridine 5′-triphosphate-N-acetylglucosamine were performed for 60 min at 32°C and the membranes subsequently digested with endo D and β-N-acetylglucosaminidase (19). Syntaxin 5-specific reagents were preincubated in complete reaction cocktails on ice for 20 min before transfer to 32°C. Processing of VSV-G to the endo D-sensitive form was followed by quantitative immune blotting (14).
-
-
-
-
45
-
-
6844244893
-
-
note
-
Vesicle formation and two-stage assays were done as described (14) with the following modifications: (i) The membranes were resuspended in 0.25 M sucrose, 10 mM Hepes (pH 7.4) before the addition of salts and differential centrifugation; (ii) the medium speed spin was increased to 5 min in the two-stage assay; and (iii) stage 2 incubations contained gel-filtered cytosol (19). Endo D digestion was performed as described above.
-
-
-
-
46
-
-
6844244894
-
-
note
-
Immunoisolated ER-derived vesicles (14) were solubilized in 0.5% Triton X-100 buffers containing either ATP and EDTA (assembly) or MgATP (disassembly) (22). For immunoprecipitation experiments, the vesicle extracts (0.4 ml) were incubated with 0.5 mg of α-SNAP and Myc-NSF immobilized on anti-Myc-coupled magnetic beads. The immunoprecipitates were washed extensively before SDS-polyacrylamide gel electrophoresis (PAGE) and immune blotting. Analysis on 10 to 40% (w/v) glycerol density gradients was done as described (40). Fractions were collected and precipitated with 10% trichloroacetic acid before SDS-PAGE and immune blotting with enhanced chemiluminescence detection.
-
-
-
-
47
-
-
6844228738
-
-
note
-
2, 20 mM Hepes (pH 7.4)], then resuspended in 4 ml of transport buffer plus PIC. The Golgi membranes were divided into 0.1-ml samples, frozen in liquid nitrogen, and stored at -80°C.
-
-
-
-
48
-
-
6844262231
-
-
note
-
We thank R. Scheller and J. Hay (Stanford University) for antibodies to mSec22b, rBet1, and membrin; J. Saraste (University of Bergen, Bergen, Norway) for anti-p58; and S. Whiteheart (University of Kentucky Medical Center) for recombinant α-SNAP, Myc-tagged NSF, and anti-NSF (6E6). Supported by NIH grant GM 42336 (to W.E.B.) and the National Cancer Institute (CA58689). Electron microscopy made extensive use of Core B in CA58689. T.R. is a recipient of a fellowship from the Muscular Dystrophy Association, C.D. is a recipient of a fellowship grant from the Deutsche Forschungsgemeinschaft, and S.B. is a recipient of a Cystic Fibrosis Foundation Fellowship.
-
-
-
|