Extended life-span and stress resistance in the Drosophila mutant methuselah

Science

Volumn 282, Issue 5390, 1998, Pages 943-946

  Lin, Yi Jyun ^a   Seroude, Laurent ^a   Benzer, Seymour ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FREE RADICAL;

ANIMAL CELL; ARTICLE; BREEDING; DISSECTION; DROSOPHILA MELANOGASTER; GENE MUTATION; LIFESPAN; NONHUMAN; PREDICTION; PRIORITY JOURNAL; STARVATION; STRESS;

AMINO ACID SEQUENCE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; FEMALE; FOOD DEPRIVATION; GENES, INSECT; GTP-BINDING PROTEINS; HEAT; INSECTICIDE RESISTANCE; LONGEVITY; MALE; MOLECULAR SEQUENCE DATA; MUTATION; OXIDATIVE STRESS; PARAQUAT; RECEPTORS, CELL SURFACE; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION;

EID: 0032582489     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5390.943     Document Type: Article

References (45)

1. M. R. Rose and B. Charlesworth, Nature 287, 141 (1980); Genetics 97, 173 (1981); ibid., p. 187.
2. M. R. Rose and B. Charlesworth, Nature 287, 141 (1980); Genetics 97, 173 (1981); ibid., p. 187.
3. M. R. Rose and B. Charlesworth, Nature 287, 141 (1980); Genetics 97, 173 (1981); ibid., p. 187.
4. D. B. Friedman and T. E. Johnson, Genetics 118, 75 (1988); S. Hekimi, P. Boutis, B. Lakowski, ibid. 141, 1351 (1995); C. Kenyon et al., Nature 366, 461 (1993).
5. D. B. Friedman and T. E. Johnson, Genetics 118, 75 (1988); S. Hekimi, P. Boutis, B. Lakowski, ibid. 141, 1351 (1995); C. Kenyon et al., Nature 366, 461 (1993).
6. D. B. Friedman and T. E. Johnson, Genetics 118, 75 (1988); S. Hekimi, P. Boutis, B. Lakowski, ibid. 141, 1351 (1995); C. Kenyon et al., Nature 366, 461 (1993).
7. J. Z. Morris, H. A. Tissenbaum, G. Ruvkun, Nature 382, 536 (1996); J. J. Ewbank et al., Science 275, 980 (1997); S. Hekimi et al., Trends Genet. 14, 14 (1998).
8. J. Z. Morris, H. A. Tissenbaum, G. Ruvkun, Nature 382, 536 (1996); J. J. Ewbank et al., Science 275, 980 (1997); S. Hekimi et al., Trends Genet. 14, 14 (1998).
9. J. Z. Morris, H. A. Tissenbaum, G. Ruvkun, Nature 382, 536 (1996); J. J. Ewbank et al., Science 275, 980 (1997); S. Hekimi et al., Trends Genet. 14, 14 (1998).
10. K. D. Kimura et al., Science 277, 942 (1997).
11. G. J. Lithgow et al., J. Gerontol. 49, B270 (1994); G. J. Lithgow et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7540 (1995).
12. G. J. Lithgow et al., J. Gerontol. 49, B270 (1994); G. J. Lithgow et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7540 (1995).
13. P. L. Larsen, Proc. Natl. Acad. Sci. U.S.A. 90, 8905 (1993); J. R. Vanfleteren, Biochem. J. 292, 605 (1993).
14. P. L. Larsen, Proc. Natl. Acad. Sci. U.S.A. 90, 8905 (1993); J. R. Vanfleteren, Biochem. J. 292, 605 (1993).
15. P. M. Service et al., Physiol. Zool. 58, 380 (1985).
16. J. L. Graves et al., ibid. 65, 268 (1992).
17. R. Arking et al., Dev. Genet. 12, 362 (1991).
18. W. C. Orr and R. S. Sohal, Science 263, 1128 (1994).
19. T. Parkes et al., Nature Genet. 19, 171 (1998).
20. L. Cooley, R. Kelley, A. Spradling, Science 239, 1121 (1988).
21. 1118 for several generations, until the color was homogeneous. Each insertion was mapped to a chromosome by the use of balancers, tested for homozygous viability and fertility, and established as an independent line. After its isolation, mth was extensively out-crossed before further characterization to avoid nonspecific effects of genetic background (26).
22. F. M. Ashton and A. S. Crafts, Mode of Action of Insecticide (Wiley Interscience, New York, 1973).
23. A. R. Heydari et al., Experientia 50, 1092 (1994); J. C. Wheeler, E. T. Bieschke, J. Tower, Proc. Natl. Acad. Sci. U.S.A. 92, 10408 (1995).
24. A. R. Heydari et al., Experientia 50, 1092 (1994); J. C. Wheeler, E. T. Bieschke, J. Tower, Proc. Natl. Acad. Sci. U.S.A. 92, 10408 (1995).
25. M. Tatar, A. A. Khazaeli, J. W. Curtsinger, Nature 390, 30 (1997).
26. The mth genomic DNA was probed by the ampicillin resistance gene contained in the P-element construct used to generate mutant lines (25).
27. + Δ2-3}. The male jump-starters were then crossed to w;TM3/TM6. Progeny with white eyes were made homozygous and lines established. Two alleles were homozygous lethal before the L2 larval stage and so were maintained over the third chromosome balancers, TM3 or TM6.
28. -137, well within the range of identical sequences. The full sequence of LD08316 (1948 nucleotides) (Fig. 3B) also corresponded to the downstream genomic sequence of mth. We then used the cDNA as a probe to isolate mth genomic DNA from a P1-phagemid grid from Genome Systems. Three P1 plasmids (DS05332, DS03799, and DS06692) from the BDPG contained the genomic region of the mth gene. These P1 clones have a common contig, DS00539, which maps at 61C on the third chromosome (BDGP database). A corresponding 7.9-kb Eco RI fragment from DS06692 was subcloned into pBluescript vector and the full-length sequence determined.
29. S. F. Altschul et al., Nucleic Acids Res. 25, 3389 (1997).
30. S. Henikoff and J. G. Henikoff, Genomics 19, 97 (1994).
31. B. K. Kobilka et al., Science 240, 1310 (1988); T. Kubo et al., FEBS Lett. 241, 119 (1988); S. K. Wong, C. Slaughter, A. E. Ruoho, E. M. Ross, J. Biol. Chem. 263, 7925 (1988); J. Ostrowski, M. A. Kjelsberg, M. G. Caron, R. J. Lefkowitz, Annu. Rev. Pharmacol. Toxicol. 32, 167 (1992); E. S. Burstein, T. A. Spalding, M. R. Brann, Biochemistry 37, 4052 (1998).
32. B. K. Kobilka et al., Science 240, 1310 (1988); T. Kubo et al., FEBS Lett. 241, 119 (1988); S. K. Wong, C. Slaughter, A. E. Ruoho, E. M. Ross, J. Biol. Chem. 263, 7925 (1988); J. Ostrowski, M. A. Kjelsberg, M. G. Caron, R. J. Lefkowitz, Annu. Rev. Pharmacol. Toxicol. 32, 167 (1992); E. S. Burstein, T. A. Spalding, M. R. Brann, Biochemistry 37, 4052 (1998).
33. B. K. Kobilka et al., Science 240, 1310 (1988); T. Kubo et al., FEBS Lett. 241, 119 (1988); S. K. Wong, C. Slaughter, A. E. Ruoho, E. M. Ross, J. Biol. Chem. 263, 7925 (1988); J. Ostrowski, M. A. Kjelsberg, M. G. Caron, R. J. Lefkowitz, Annu. Rev. Pharmacol. Toxicol. 32, 167 (1992); E. S. Burstein, T. A. Spalding, M. R. Brann, Biochemistry 37, 4052 (1998).
34. B. K. Kobilka et al., Science 240, 1310 (1988); T. Kubo et al., FEBS Lett. 241, 119 (1988); S. K. Wong, C. Slaughter, A. E. Ruoho, E. M. Ross, J. Biol. Chem. 263, 7925 (1988); J. Ostrowski, M. A. Kjelsberg, M. G. Caron, R. J. Lefkowitz, Annu. Rev. Pharmacol. Toxicol. 32, 167 (1992); E. S. Burstein, T. A. Spalding, M. R. Brann, Biochemistry 37, 4052 (1998).
35. B. K. Kobilka et al., Science 240, 1310 (1988); T. Kubo et al., FEBS Lett. 241, 119 (1988); S. K. Wong, C. Slaughter, A. E. Ruoho, E. M. Ross, J. Biol. Chem. 263, 7925 (1988); J. Ostrowski, M. A. Kjelsberg, M. G. Caron, R. J. Lefkowitz, Annu. Rev. Pharmacol. Toxicol. 32, 167 (1992); E. S. Burstein, T. A. Spalding, M. R. Brann, Biochemistry 37, 4052 (1998).
36. G. A. Walker, D. W. Walker, G. J. Lithgow, Ann. N.Y. Acad. Sci. 851, 444 (1998).
37. S. Watson and S. Arkinstall, The G-protein Linked Receptor Facts Book (Academic Press, London, 1994); G. Vassart et al., Ann. N.Y. Acad. Sci. 766, 23 (1995); T. P. Sakmar, Prog. Nucleic Acid Res. Mol. Biol. 59, 1 (1998).
38. S. Watson and S. Arkinstall, The G-protein Linked Receptor Facts Book (Academic Press, London, 1994); G. Vassart et al., Ann. N.Y. Acad. Sci. 766, 23 (1995); T. P. Sakmar, Prog. Nucleic Acid Res. Mol. Biol. 59, 1 (1998).
39. S. Watson and S. Arkinstall, The G-protein Linked Receptor Facts Book (Academic Press, London, 1994); G. Vassart et al., Ann. N.Y. Acad. Sci. 766, 23 (1995); T. P. Sakmar, Prog. Nucleic Acid Res. Mol. Biol. 59, 1 (1998).
40. E. Bier et al., Genes Dev. 3, 1273 (1989).
41. J. Tower, Bioessays 18, 799 (1996).
42. B. A. Hamilton and K. Zinn, in Methods in Cell Biology, L. S. B. Goldstein and E. A. Fryberg, Eds. (Academic Press, San Diego, 1994), vol. 44, p. 81.
43. S. F. Altschul et al., J. Mol. Biol. 215, 403 (1990).
44. J. Kyte and R. F. Doolittle, ibid. 157, 105 (1982).
45. Supported by a Postdoctoral Research Fellowship from the John Douglas French Alzheimer Foundation (Y.-J.L.) and grants from the National Science Foundation (MCB9408718), the National Institutes of Health (EY09278 and AG12289), and the James G. Boswell Foundation (S.B.).