GnRH signalling pathways and GnRH-induced homologous desensitization in a gonadotrope cell line (αT3-1)

Molecular and Cellular Endocrinology

Volumn 142, Issue 1-2, 1998, Pages 99-117

  Poulin, B ^a,b   Rich, N ^a   Mas, J L ^b   Kordon, C ^a   Enjalbert, A ^a,b   Drouva, S V ^a,b  

^a CENTRE PAUL BROCA   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

T3 1 cells; Arachidonic acid; Down regulation; G proteins; GnRH; Gonadotrope cell line; Homologous desensitization; Inositol phosphates; Phospholipase A2; Phospholipase C; Phospholipase D; Protein kinase C isoenzymes

Indexed keywords

ARACHIDONIC ACID; GONADORELIN; GONADORELIN DERIVATIVE; INOSITOL PHOSPHATE; INOSITOL TRISPHOSPHATE; PHORBOL ESTER; PHOSPHATIDYLETHANOL; PHOSPHATIDYLINOSITIDE; PHOSPHOLIPASE A2; PHOSPHOLIPASE C; PHOSPHOLIPASE D; PROTEIN KINASE C; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; DESENSITIZATION; DOWN REGULATION; ENZYME ACTIVATION; GONADOTROPIN SECRETING CELL; HYDROLYSIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TIME;

ARACHIDONIC ACID; CELL LINE; DRUG TOLERANCE; ENZYME ACTIVATION; GLYCEROPHOSPHOLIPIDS; GONADOTROPIN-RELEASING HORMONE; GONADOTROPINS, PITUITARY; INOSITOL 1,4,5-TRISPHOSPHATE; INOSITOL PHOSPHATES; ISOENZYMES; KINETICS; PHOSPHATIDYLINOSITOLS; PHOSPHOLIPASE C; PHOSPHOLIPASE D; PHOSPHOLIPASES A; PITUITARY GLAND; SIGNAL TRANSDUCTION; SODIUM FLUORIDE; TETRADECANOYLPHORBOL ACETATE;

EID: 0032566156     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(98)00114-2     Document Type: Article

References (65)

1. Akhtar R.A., Taft W.C., Abdel-Latif A.A. Effects of corticotropin-(1-24)-tetracosapeptide on polyphosphoinositide metabolism and protein phosphorylation in rabbit iris subcellular fractions. J. Neurochem. 41:1983;1460-1468.
2. Anderson L., Milligan G., Eidne K.A. Characterization of the gonadotrophin-releasing hormone receptor in αT3-1 pituitary gonadotroph cells. J. Endocrinol. 136:1993;51-58.
3. Anderson L., McGregor A., Cook J.V., Chilvers E., Eidne K.A. Rapid desensitization of GnRH-stimulated intracellular signalling events in αT3-1 and HEK-293 cells expressing the GnRH receptor. Endocrinology. 136:1995;5228-5231.
4. Bartlett G.R. Phosphorus assay in column chromatography. J. Biol. Chem. 234:1959;466-468.
5. Ben-Menahem D., Shraga-Levine Z., Limor R., Naor Z. Arachidonic acid and lipoxygenase products stimulate gonadotropin α-subunit mRNA levels in pituitary αT3-1 cell line: role in gonadotropin releasing hormone action. Biochemistry. 33:1994;12795-12799.
6. Benya R.V., Kusui T., Battey J.F., Jensen R.T. Chronic desensitization and down-regulation of the gastrin-releasing peptide receptor are mediated by a protein kinase C-dependent mechanism. J. Biol. Chem. 270:1995;3346-3352.
7. Chuang T.T., Iacovelli L., Sallese M., DeBlasi A. G protein-coupled receptors: heterologous regulation of homologous desensitization and its implications. Trends Pharmacol. Sci. 17:1996;416-419.
8. Cubitt A.B., Zhang B., Gershengorn M.C. Analysis by base exchange of Thyrotropin-releasing hormone responsive and unresponsive inositol lipids pools in rat pituirary tumor cells. J. Biol. Chem. 265:1990;9707-9714.
9. Davidson J.S., Wakefield I.K., Millar R.P. Absence of rapid desensitization of the mouse gonadotropin-releasing hormone receptor. Biochem. J. 300:1994;299-302.
10. 1 adrenergic receptor coupling with phospholipase-C is negatively regulated by protein kinase-C in primary cultures of hypothalamic neurons and glial cells. Endocrinology. 129:1991;1605-1613.
11. Drouva, S.V., Poulin, B., Mas, J.L., Enjalbert, A., 1997. Differential down-regulation of PKC isoenzymes by GnRH and TPA on αT3-1 gonadotrope cell line. 27th meeting of the Society for Neuroscience, New Orleans, p. 210.
12. Eidne K.A., Sellar R.E., Couper G., Anderson L., Taylor P.L. Molecular cloning and characterisation of the Rat pituitary gonadotropin-releasing hormone (GnRH) receptor. Mol. Cell. Endocrinol. 90:1992;R5-R9.
13. Exton J.H. New developments in phospholipase D. J. Biol. Chem. 272:1997;15579-15582.
14. Freedman N.J., Lefkowitz R.J. Desensitization of G protein-coupled receptors. Rec. Prog. Horm. Res. 51:1996;319-353.
15. Folsh J., Lees M., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:1957;497-509.
16. Gautron J.-P., Poulin B., Kordon C., Drouva S.V. Characterization of (hydroxyproline(9)) luteinizing hormone-releasing hormone and its smallest precursor forms in immortalized luteinizing hormone-releasing hormone-secreting neurons (GT1-7), and evaluation of their mode of action on pituitary cells. Mol. Cell. Endocrinol. 110:1995;161-173.
17. 2 enzymes: regulation and inhibition. Trends Pharmacol. Sci. 14:1993;92-98.
18. Gorospe W.C., Conn P.M. Agents that decrease gonadotropin-releasing hormone (GnRH) receptor internalization do not inhibit GnRH-mediated gonadotrope desensitization. Endocrinology. 120:1987;222-229.
19. Harris D., Reiss N., Naor Z. Differential activation of protein kinase C δ and ε gene expression by gonadotropin-releasing hormone in αT3-1 cells. Autoregulation by protein kinase C. J. Biol. Chem. 272:1997;13534-13540.
20. Hawes B.E., Conn P.M. Development of gonadotrope desensitization to gonadotropin-releasing hormone (GnRH) and recovery are not coupled to inositol phosphate production or GnRH number. Endocrinology. 131:1992;2681-2689.
21. Hipkin R.W., Friedman J., Clark R.B., Eppler C.M., Shonbrunn A. Agonist-induced desensitization, internalization, and phosphorylation of the sst2A somatostatin receptor. J. Biol. Chem. 272:1997;13869-13876.
22. 11. Mol. Endocrinol. 6:1992;1673-1681.
23. Johnson M.S., MacEwan D.J., Simpson J., Mitchell R. Characterisation of protein kinase C isoforms and enzymic activity from the αT3-1 gonadotroph-derived cell line. FEBS Lett. 333:1993;67-72.
24. Katt J.A., Duncan J.A., Herbon L., Barkan A., Marshall J.C. The frequency of gonadotropin-releasing hormone stimulation determines the number of pituitary gonadotropin-releasing hormone receptors. Endocrinology. 116:1985;2113-2115.
25. Kay T.W.H., Chedrese P.J., Jameson J.L. Gonadotropin-releasing hormone causes transcriptional stimulation followed by desensitization of the glycoprotein hormone α promoter in transfected αT3 gonadotrope cells. Endocrinology. 134:1994;568-573.
26. Koelle M.R. A new family of G-protein regulators-the RGS proteins. Curr. Opin. Cell. Biol. 9:1997;143-147.
27. Kratzmeier M., Poch A., Mukhopadhyay A.K., McArdle C.A. Selective translocation of non-conventional protein kinase C isoenzymes by gonadotropin-releasing hormone (GnRH) in the gonadotrope-derived αT3-1 cell line. Mol. Cell. Endocrinol. 118:1996;103-111.
28. 2-adrenergic receptor dephosphorylation by vesicular acidification. J. Biol. Chem. 272:1997;5-8.
29. 2. J. Biol. Chem. 272:1997;16709-16712.
30. Limatola C., Schaap D., Moolenaar W.H., VanBlitterswijk W.J. Phosphatidic acid activation of protein kinase C-ζ overexpressed in COS cells: comparison with other protein kinase C isotypes and other acidic lipids. Biochem. J. 304:1994;1001-1008.
31. Liu J.-P. Protein kinase C and its substrates. Mol. Cell. Endocrinol. 116:1996;1-29.
32. Loumaye E., Catt K.J. Homologous regulation of gonadotropin-releasing hormone receptors in cultured pituitary cells. Science. 215:1982;983-985.
33. Loumaye E., Catt K.J. Agonist-induced regulation of pituitary receptors for gonadotropin-releasing hormone. Dissociation of receptor recruitment from hormone release in cultured gonadotrophs. J. Biol. Chem. 258:1983;12002-12009.
34. Loumaye E., Wynn P.C., Coy D., Catt K.J. Receptor-binding properties of gonadotropin-releasing hormone derivatives. Prolonged receptor occupancy and cell-surface localisation of a potent antagonist analog. J. Biol. Chem. 259:1984;12663-12671.
35. Marshall J.C., Dalkin A.C., Haisenleder D.J., Paul S.J., Ortolano G.A., Kelch R.P. Gonadotropin-releasing hormone pulses: regulators of gonadotropin synthesis and ovulatory cycles. Rec. Prog. Horm. Res. 47:1991;155-187.
36. Mason D.R., Arora K.K., Mertz L.M., Catt K.J. Homologous down-regulation of gonadotropin-releasing hormone receptor sites and messenger ribonucleic acid transcripts in αT3-1 cells. Endocrinology. 135:1994;1165-1170.
37. McArdle C.A., Gorospe W.C., Huckle W.R., Conn P.M. Homologous down-regulation of gonadotropin-releasing hormone receptors and desensitization of gonadotropes: lack of dependence on protein kinase C. Mol. Endocrinol. 1:1987;420-429.
38. McArdle C.A., Forrest-Owen W., Willars G., Davidson J., Poch A., Kratzmeier M. Desensitization of gonadotropin-releasing hormone action in the gonadotrope-derived αT3-1 cell line. Endocrinology. 136:1995;4864-4871.
39. 2+-binding site in the mechanism of inhibition. J. Biol. Chem. 272:1997;10474-10482.
40. 2+ mobilizing hormones: the case of gonadotropin-releasing hormone. Endocr. Rev. 11:1990;326-353.
41. Naor Z. Is arachidonic acid a second messenger in signal transduction. Mol. Cell. Endocrinol. 80:1991;C181-C186.
42. Neill J.D., Musgrove L.C., Sellers J.C., Duck L.W. Identification by polymerase chain reaction of the G protein-coupled receptor kinases expressed in the mouse gonadotropic αT3-1 cell line. Endocrinology. 137:1996;3942-3947.
43. Neill J.D., Duck L.W., Sellers J.C., Musgrove L.C., Scheschonka A., Druey K.M., Kehrl J.H. Potential role of a regulator of G protein signaling (RGS3) in gonadotropin-releasing hormone (GnRH) stimulated desensitization. Endocrinology. 138:1997;843-846.
44. Ozawa K., Yamada K., Kazanietz M.G., Blumberg P.M., Beaven M.A. Different isoenzymes of protein kinase C mediate feedback inhibition of phospholipase C and stimulatory signals for exocytosis in rat RBL-2H3 cells. J. Biol. Chem. 268:1993;2280-2283.
45. Pachter J.A., Pai J.-K., Mayer-Ezell R., Petrin J.M., Dobek E., Bishop W.R. Differential regulation of phosphoinositide and phosphatidylcholine hydrolysis by protein kinase C-β1 overexpression. Effects on stimulation by α-thrombin, guanosine 5′-O-(thiotriphosphate), and calcium. J. Biol. Chem. 267:1992;9826-9830.
46. Parker J., Daniel L.W., Waite M. Evidence of protein kinase C involvement in phorbol diester-stimulated arachidonic acid release and prostaglandin synthesis. J. Biol. Chem. 262:1987;5385-5393.
47. Poulin, B., Bojti, T., Mitev, Y., Kordon, C., Enjalbert, A., Drouva, S.V., 1995. Implication des mécanismes de couplage du récepteur du GnRH avec les phospholipases C, A2 et D dans la désensibilisation induite par le GnRH dans une lignée cellulaire de type gonadotrope (αT3-1). 2nd Colloque de la Société des Neurosciences, Lyon, abstract G44, p. 264.
48. 2 and -D activation in a gonadotrope cell line (αT3-1). Mol. Cell. Endocrinol. 122:1996;33-50.
49. Rhee S.G., Bae Y.S. Regulation of phosphoinositide-specific phospholipase C isoenzymes. J. Biol. Chem. 272:1997;15045-15048.
50. Reinhart J., Mertz L.M., Catt K.J. Molecular cloning and expression of cDNA encoding the murine gonadotropin-releasing hormone receptor. J. Biol. Chem. 267:1992;21281-21284.
51. Salmon J.A., Flower R.J. Extraction and thin-layer chromatography of arachidonic acid metabolites. Methods Enzymol. 86:1982;477-493.
52. 11α (pertussis toxin-insensitive) G proteins in αT3-1 gonadotroph cells reflects increased G protein turnover but not alterations in mRNA levels. Proc. Natl. Acad. Sci. USA. 92:1995;1886-1890.
53. Smith M.A., Vale W.W. Desensitization to gonadotropin-releasing hormone observed in superfused pituitary cells on cytodex beads. Endocrinology. 108:1981;752-759.
54. q. Science. 251:1991;804-807.
55. q/11α by the gonadotrope receptor. Mol. Endocrinol. 11:1997;738-746.
56. Stojilkovic S.S., Rojas E., Stutzin A., Izumi S.-I., Catt K.J. Desensitization of pituitary gonadotropin secretion by agonist-induced inactivation of voltage-sensitive calcium channels. J. Biol. Chem. 264:1989;10939-10942.
57. Stojilkovic S.S., Reinhart J., Catt K.J. Gonadotropin-releasing hormone receptors: structure and signal transduction pathways. Endocr. Rev. 15:1994;462-499.
58. Tsutsumi M., Zhou W., Millar R.P., Mellon P.L., Roberts J.L., Flanagan C.A., Dong K., Gillo B., Sealfon S.C. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6:1992;1163-1169.
59. Tsutsumi M., Laws S.C., Rodic V., Sealfon S.C. Translational regulation of the gonadotropin-releasing hormone receptor in αT3-1 cells. Endocrinology. 136:1995;1128-1136.
60. 3 pituitary cells. Am. J. Physiol. 257:1989;E203-E211.
61. Weiss J., Duca K.A., Crowley W.F. Gonadotropin-releasing hormone-induced stimulation and desensitization of free α-subunit secretion mirrors luteinizing hormone and follicle-stimulating hormone in perifused rat pituitary cells. Endocrinology. 127:1990;2364-2371.
62. Weiss J., Cote C.R., Jameson J.L., Crowley W.F. Homologous desensitization of gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone secretion in vitro occurs within the duration of an endogenous GnRH pulse. Endocrinology. 136:1995;138-143.
63. Windle J.J., Weiner R.I., Mellon P.L. Cell lines of the pituirary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Mol. Endocrinol. 4:1990;597-603.
64. Zheng L., Stojilkovic S.S., Hunyady L., Krsmanovic L.Z., Catt K.J. Sequential activation of phospholipase-C and -D in agonist-stimulated gonadotrophs. Endocrinology. 134:1994;1446-1454.
65. Zhou G., Wooten M.W., Coleman E.S. Regulation of atypical ζ-protein kinase C in cellular signaling. Exp. Cell. Res. 214:1994;1-11.