Rapid isolation of RNA polymerase from sporulating cells of Bacillus subtilis

Gene

Volumn 221, Issue 2, 1998, Pages 185-190

  Fujita, Masaya ^a   Sadaie, Yoshito ^a  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

Author keywords

' subunit; Hexahistidine tag; Nickel chelate chromatography; Sigma factor; Transcription

Indexed keywords

HISTIDINE; MUTANT PROTEIN; RNA POLYMERASE; SIGMA FACTOR;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIAL SPORE; ENZYME PURIFICATION; ENZYME SUBUNIT; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SPOROGENESIS;

BACILLUS SUBTILIS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMOSOMES, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; HISTIDINE; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPORES, BACTERIAL; TRANSCRIPTION, GENETIC; TRANSFORMATION, BACTERIAL;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0032561518     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00452-1     Document Type: Article

References (24)

1. Babcock M.J., Buttner M.J., Keler C.H., Clarke B.R., Morris R.A., Lewis C.G., Brawner M.E. Characterization of the rpoC gene of Streptomyces coelicolor A3(2) and its use to develop a simple and rapid method for the purification of RNA polymerase. Gene. 196:1997;31-42.
2. Baldus J.M., Buckner C.M., Moran C.P. Jr. Evidence that the transcription activator Spo0A interacts with two sigma factors in Bacillus subtilis. Mol. Microbiol. 17:1995;281-290.
3. Cutting S., Panzer S., Losick R. Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacilus subtilis. J. Mol. Biol. 207:1989;393-404.
4. H holoenzymes. J. Biochem. (Tokyo). 124:1998;89-97.
5. Fujita M., Sadaie Y. Feedback loops involving Spo0A and AbrB in in vitro transcription of the genes involved in the initiation of sporulation in Bacillus subtilis. J. Biochem. (Tokyo). 124:1998;98-104.
6. Haldenwang W.G. The sigma factors of Bacillus subtilis. Microbiol. Rev. 59:1995;1-30.
7. Hicks K.A., Grossman A.D. Altering the level and regulation of the major sigma subunit of RNA polymerase affects gene expression and development in Bacillus subtilis. Mol. Microbiol. 20:1996;201-212.
8. Ishihama A. Adaptation of gene expression in stationary phase bacteria. Curr. Opin. Genet. Dev. 7:1997;582-588.
9. Itaya M., Kondo K., Tanaka T. A neomycin resistance gene cassette selectable in a single copy state in the Bacilus subtilis chromosome. Nucleic Acids Res. 17:1989;4410.
10. 38. J. Bacteriol. 177:1995;6832-6835.
11. Kashlev M., Martin E., Polyakov A., Severinov K., Nikiforov V., Goldfarb A. Histidine-tagged RNA polymerase: dissection of the transcription cycle using immobilized enzyme. Gene. 130:1993;9-14.
12. Kemp E.H., Sammons R.L., Moir A., Sun D., Setlow P. Analysis of transcriptional control of the gerD spore germination gene of Bacillus subtilis 168. J. Bacteriol. 173:1991;4646-4652.
13. Kunst F.et al. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature. 390:1997;249-256.
14. Leighton T.J., Doi R.H. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J. Biol. Chem. 254:1971;3189-3195.
15. Linn T.G., Greenleaf A.L., Shorenstein R.G., Losick R. Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. Proc. Natl. Acad. Sci. USA. 70:1973;1865-1869.
16. E-associated RNA polymerase. J. Bacteriol. 175:1993;4081-4086.
17. H protein, in Bacilus subtilis. Mol. Microbiol. 29:1998;505-513.
18. Perego M., Spiegelman G.B., Hoch J.A. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol. Microbiol. 2:1988;689-699.
19. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
20. F-dependent promoters. J. Bacteriol. 173:1991;7867-7874.
21. Tang H., Severinov K., Goldfarb A., Ebright R.H. Rapid RNA polymerase genetics: one-day, no column preparation of reconstituted recombinant Escherichia coli RNA polymerase. Proc. Natl. Acad. Sci. USA. 92:1995;4902-4906.
22. Tjian R., Losick R. An immunological assay for the sigma subunit of RNA polymerase in extracts of vegetative and sporulating Bacillus subtilis. Proc. Natl. Acad. Sci. USA. 71:1974;2872-2876.
23. Yang X., Price C.W. Streptolydigin resistance can be conferred by alterations to either the β or β′ subunits of Bacillus subtilis RNA polymerase. J. Biol. Chem. 270:1995;23930-23933.
24. Zuber P., Healy J., Carter III H.L., Cutting S., Moran C.P. Jr., Losick R. Mutation changing the specificity of an RNA polymerase sigma factor. J. Mol. Biol. 206:1989;605-614.