Characterization of the rpoC gene of Streptomyces coelicolor A3(2) and its use to develop a simple and rapid method for the purification of RNA polymerase

Gene

Volumn 196, Issue 1-2, 1997, Pages 31-42

  Babcock, Martin J ^a,b   Buttner, Mark J ^c,d   Keler, Cynthia H ^a   Clarke, Belinda R ^c,d   Morris, René A ^c   Lewis, Cinzia G ^c,d   Brawner, Mary E ^a  

^a Depts Gene Expression Sci M   (United States)

^b ABBOTT LABORATORIES   (United States)

^c JOHN INNES CENTRE   (United Kingdom)

^d UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

' subunit; Hexa histidine tag; Nickel chelate chromatography; Phylogeny; Transcription

Indexed keywords

HISTIDINE; RIFAMPICIN; RNA POLYMERASE;

AFFINITY CHROMATOGRAPHY; ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BETA CHAIN; CHROMOSOME; DNA HYBRIDIZATION; ESCHERICHIA COLI; GENE ISOLATION; GENE MUTATION; GENETIC MARKER; GENETIC TRANSCRIPTION; MYCOBACTERIUM LEPRAE; MYCOPLASMA; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; SPOROGENESIS; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS; TRANSLATION INITIATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; HISTIDINE; MOLECULAR SEQUENCE DATA; MUTATION; NICKEL; PLANT PROTEINS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM LEPRAE; MYCOPLASMA; MYCOPLASMA GENITALIUM; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS;

EID: 0030864767     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00179-0     Document Type: Article

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