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Volumn 119, Issue 14, 1997, Pages 3245-3252

Controlling subtilisin activity and selectivity in organic media by imprinting with nucleophilic substrates

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SPECIFICITY; MOLECULAR DYNAMICS; THERMODYNAMICS;

EID: 0030908708     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9637715     Document Type: Article
Times cited : (84)

References (63)
  • 24
    • 0025485121 scopus 로고
    • (b) Arnold, F. H. TIBTECH 1990, 8, 244-249.
    • (1990) TIBTECH , vol.8 , pp. 244-249
    • Arnold, F.H.1
  • 43
    • 1842329365 scopus 로고
    • TRIPOS Associates: St. Louis, MO
    • SYBYL; TRIPOS Associates: St. Louis, MO, 1993.
    • (1993) SYBYL
  • 45
    • 1842365113 scopus 로고    scopus 로고
    • note
    • The 5′-butyrate was synthesized using subtilisin Carlsberg to generate both the 5′- and 3′-esters followed by silica gel flash column chromatography purification of the 5′-ester. The 3′-ester was synthesized Similar Lipase PS30 and purified by flash column silica gel chromatography. Similar reactions and purifications were performed with the other deoxynucleosides employed in this study.
  • 50
    • 0018094892 scopus 로고
    • (a) Perutz, M. Science 1978, 201, 1187-1191.
    • (1978) Science , vol.201 , pp. 1187-1191
    • Perutz, M.1
  • 52
    • 1842325832 scopus 로고    scopus 로고
    • note
    • The dipole moment for thymidine was calculated using the Pullman charge calculation method within SYBYL. The SYBYL command COMPUTE...DIPOLE was used to determine the dipole moment.
  • 56
    • 1842414459 scopus 로고    scopus 로고
    • note
    • 1′ binding pocket. Indeed, the observed and predicted regioselectivities for thymidine acylation catalyzed by various subtilisin BPN′ mutants were in close agreement. For example, the M222Q enzyme had observed and predicted 5′-butyrate/3′-butyrate ratios of 1.93 and 1.77, respectively.
  • 58
    • 0023729499 scopus 로고
    • McPhalen, C. A.; James, M. N. G. Biochemistry 1988, 27, 6582-6598. This paper presents the highest resolution (2.2 Å) crystal structure of subtilisin BPN′ complexed with an inhibitor currently available in the Brookhaven Protein Data Bank (entry 2sni).
    • (1988) Biochemistry , vol.27 , pp. 6582-6598
    • McPhalen, C.A.1    James, M.N.G.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.