Bloodstream- versus tick-associated variants of a relapsing fever bacterium

Science

Volumn 280, Issue 5371, 1998, Pages 1938-1940

  Schwan, Tom G ^a   Hinnebusch, B Joseph ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BORRELIA; BORRELIA INFECTION; DISEASE CARRIER; HUMAN; MOUSE; NONHUMAN; PRIORITY JOURNAL; SEROTYPE; TICK;

ANIMALS; ANTIGENIC VARIATION; ANTIGENS, BACTERIAL; ANTIGENS, SURFACE; ARACHNID VECTORS; BACTEREMIA; BACTERIAL OUTER MEMBRANE PROTEINS; BORRELIA; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION; GENES, BACTERIAL; MICE; MICE, INBRED BALB C; RELAPSING FEVER; SALIVARY GLANDS; SEROTYPING; TICKS;

ACARI; BACTERIA (MICROORGANISMS); BORRELIA HERMSII; MAMMALIA; ORNITHODOROS; SPIROCHAETALES;

EID: 0032546854     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5371.1938     Document Type: Article

References (44)

1. O. Felsenfeld, Borrelia: Strains, Vectors, Human and Animal Borreliosis (Green, St. Louis, MO, 1971).
2. T. G. Schwan, W. Burgdorfer, P. A. Rosa, in Manual of Clinical Microbiology, P. R. Murray, Ed, (American Society for Microbiology, Washington, DC, 1995), pp. 626-635.
3. M. S. Dworkin et al., Clin. Infect. Dis. 26, 122 (1998).
4. P. A. Barstad, J. E. Coligan, M. G. Raum, A. G. Barbour, J. Exp. Med. 161, 1302(1985); D. Cadavid, P. M. Pennington, T. A. Kerentseva, S. Bergström, A. G. Barbour, Infect. Immun. 65, 3352 (1997).
5. P. A. Barstad, J. E. Coligan, M. G. Raum, A. G. Barbour, J. Exp. Med. 161, 1302(1985); D. Cadavid, P. M. Pennington, T. A. Kerentseva, S. Bergström, A. G. Barbour, Infect. Immun. 65, 3352 (1997).
6. T. Kitten and A. G. Barbour, Proc. Natl. Acad. Sci. U.S.A. 87, 6077 (1990).
7. J. T. Meier, M. I. Simon, A. G. Barbour, Cell 41, 403 (1985); R. H. A. Plasterk, M. I. Simon, A. G. Barbour, Nature 318, 257 (1985); B. I. Restrepo and A. G. Barbour, Cell 78, 867 (1994); B. I. Restrepo, C. J. Carter, A. G. Barbour, Mol. Microbiol. 13, 287 (1994).
8. J. T. Meier, M. I. Simon, A. G. Barbour, Cell 41, 403 (1985); R. H. A. Plasterk, M. I. Simon, A. G. Barbour, Nature 318, 257 (1985); B. I. Restrepo and A. G. Barbour, Cell 78, 867 (1994); B. I. Restrepo, C. J. Carter, A. G. Barbour, Mol. Microbiol. 13, 287 (1994).
9. J. T. Meier, M. I. Simon, A. G. Barbour, Cell 41, 403 (1985); R. H. A. Plasterk, M. I. Simon, A. G. Barbour, Nature 318, 257 (1985); B. I. Restrepo and A. G. Barbour, Cell 78, 867 (1994); B. I. Restrepo, C. J. Carter, A. G. Barbour, Mol. Microbiol. 13, 287 (1994).
10. J. T. Meier, M. I. Simon, A. G. Barbour, Cell 41, 403 (1985); R. H. A. Plasterk, M. I. Simon, A. G. Barbour, Nature 318, 257 (1985); B. I. Restrepo and A. G. Barbour, Cell 78, 867 (1994); B. I. Restrepo, C. J. Carter, A. G. Barbour, Mol. Microbiol. 13, 287 (1994).
11. H. G. Stoenner, T. Dodd, C. Larsen, J. Exp. Med. 156, 1297 (1982).
12. P. M. Southern and J. P. Sanford, Medicine 48, 129 (1968); A. G. Barbour and S. F. Hayes, Microbiol. Rev. 50, 381 (1986).
13. P. M. Southern and J. P. Sanford, Medicine 48, 129 (1968); A. G. Barbour and S. F. Hayes, Microbiol. Rev. 50, 381 (1986).
14. 7 spirochetes per milliliter. At this time, bloods smears were again made for serotype determination and another 75 second-stage nymphs were fed to repletion. Both cohorts of ticks were held at 27°C and 80% relative humidity. Immediately after feeding, infections in two ticks from each group were confirmed by darkfield microscopic examination of the midgut. Spirochetes in the blood smears from both mice used to infect ticks were examined with 25 serotype-specific antisera (7), which identified the initial infecting population as serotype 7 and the relapse as serotype 8.
15. Ticks were allowed to molt, and then from 21 to 372 days after infection, ticks from each cohort were fed singly on individual mice that were 10 days old. Blood from each mouse was examined daily for 2 weeks, and the serotype of the spirochetes in the initial population was determined (7, 9).
16. B. J. Hinnebusch and T. G. Schwan, unpublished data.
17. 7 spirochetes per milliliter of blood and then held at 27°C until the nymphs had molted to adults. The identity of the serotypes 7 and 8 in the mouse blood used to infect ticks was again confirmed by IFA.
18. After the molt of the nymphs to adults, we dissected the salivary glands, midgut, and synganglion for immunofluorescence analysis. Tick tissues were mixed in phosphate-buffered saline containing 0.75% bovine serum albumin, adhered to glass microscope slides, air dried, and fixed in acetone for 30 min. Spirochetes were visualized by incubation with monoclonal antibodies to flagellin (H9724) (30), Vmp7 (H9236) (31), or Vmp33 (H4825) (31), or a mouse polyclonal antibody to Vmp8 (7). This was followed by incubation with goat antibody to mouse immunoglobulin G conjugated with fluorescein isothiocyanate (Kirkegaard and Perry Laboratories, Gaithersburg, MD).
19. A. G. Barbour, S. L. Tessier, H. G. Stoenner, J. Exp. Med. 156, 1312 (1982).
20. C. J. Carter, S. Bergström, S. J. Norris, A. G. Barbour, Infect. Immun. 62, 2792 (1994).
21. 8 spirochetes per milliliter of blood. Infected blood from this mouse was cultured in BSK-H medium (Sigma, St. Louis) at 23° or 37°C until spirochetes reached stationary phase and then prepared for analysis of whole-cell lysates.
22. U. K. Laemmli, Nature 227, 680 (1970).
23. T. G. Schwan, K. K. Kime, M. E. Schrumpf, J. E. Coe, W. J. Simpson, Infect. Immun. 57, 3445 (1989).
24. H. Towbin, T. Staehelin, J. Gordon, Proc. Natl. Acad. Sci. U.S.A. 76, 4350 (1979).
25. A. G. Barbour, C. C. Carter, C. S. Freitag, T. Kitten, M. Schrumpf, unpublished data.
26. A. G. Barbour and H. G. Stoenner, in Genome Rearrangement, UCLA Symposia on Molecular and Cellular Biology, New Series, I. Herskowitz and M. I. Simon, Eds. (Liss, New York, 1985), vol. 20, pp. 123-135.
27. S. F. Porcella, B. J. Hinnebusch, D. E. Anderson Jr., T. G. Schwan, unpublished data.
28. R. T. Marconi, D. S. Samuels, T. G. Schwan, C. T. Garon, J. Clin. Microbiol. 31, 2577 (1993); N. Margolis, D. Hogan, W. Cieplak Jr., T. G. Schwan, P. A. Rosa, Gene 143, 105 (1994); B. Wilske et al., Infect. Immun. 61, 2182 (1993).
29. R. T. Marconi, D. S. Samuels, T. G. Schwan, C. T. Garon, J. Clin. Microbiol. 31, 2577 (1993); N. Margolis, D. Hogan, W. Cieplak Jr., T. G. Schwan, P. A. Rosa, Gene 143, 105 (1994); B. Wilske et al., Infect. Immun. 61, 2182 (1993).
30. R. T. Marconi, D. S. Samuels, T. G. Schwan, C. T. Garon, J. Clin. Microbiol. 31, 2577 (1993); N. Margolis, D. Hogan, W. Cieplak Jr., T. G. Schwan, P. A. Rosa, Gene 143, 105 (1994); B. Wilske et al., Infect. Immun. 61, 2182 (1993).
31. R. S. Lane, J. Piesman, W. Burgdorfer, Annu. Rev. Entomol. 36, 587 (1991).
32. T. G. Schwan, J. Piesman, W. T. Golde, M. C. Dolan, P. A. Rosa, Proc. Natl. Acad. Sci. U.S.A. 92, 2909 (1995); J. L. Coleman et al., Cell 89, 1111 (1997).
33. T. G. Schwan, J. Piesman, W. T. Golde, M. C. Dolan, P. A. Rosa, Proc. Natl. Acad. Sci. U.S.A. 92, 2909 (1995); J. L. Coleman et al., Cell 89, 1111 (1997).
34. J. Piesman, T. M. Mather, R. J. Sinsky, A. Spielman, J. Clin. Microbiol. 25, 557 (1987); J. M. C. Ribeiro, T. N. Mather, J. Piesman, A. Spielman, J. Med. Entomol. 24, 201 (1987); J. L. Zung et al., Can. J. Zool. 67, 1737 (1989).
35. J. Piesman, T. M. Mather, R. J. Sinsky, A. Spielman, J. Clin. Microbiol. 25, 557 (1987); J. M. C. Ribeiro, T. N. Mather, J. Piesman, A. Spielman, J. Med. Entomol. 24, 201 (1987); J. L. Zung et al., Can. J. Zool. 67, 1737 (1989).
36. J. Piesman, T. M. Mather, R. J. Sinsky, A. Spielman, J. Clin. Microbiol. 25, 557 (1987); J. M. C. Ribeiro, T. N. Mather, J. Piesman, A. Spielman, J. Med. Entomol. 24, 201 (1987); J. L. Zung et al., Can. J. Zool. 67, 1737 (1989).
37. S. L. Hajduk and K. Vickerman, Parasitology 83, 609 (1981); M.-F. Delauw et al., EMBO J. 4, 989 (1985).
38. S. L. Hajduk and K. Vickerman, Parasitology 83, 609 (1981); M.-F. Delauw et al., EMBO J. 4, 989 (1985).
39. B. J. Hinnebusch, A. G. Barbour, B. I. Restrepo, T. G. Schwan, Infect. Immun. 66, 432 (1998).
40. R. Kelly, Science 173, 443 (1971); A. G. Barbour, Yale J. Biol. Med. 57, 521 (1984).
41. R. Kelly, Science 173, 443 (1971); A. G. Barbour, Yale J. Biol. Med. 57, 521 (1984).
42. A. G. Barbour, S. F. Hayes, R. A. Heiland, M. E. Schrumpf, S. L. Tessier, Infect. Immun. 52, 549 (1986).
43. A. G. Barbour, in Contributions to Microbiology and Immunology, J. M. Cruse and R. E. Lewis Jr., Eds. (Karger, Basel, Switzerland, 1987), vol. 8, pp. 125-137.
44. We thank M. Schrumpf and R. Karstens for technical assistance, R. Evans and G. Hettrick for help with the figures, and P. Rosa, B. Stevenson, and J. Bono for reviewing the manuscript. The use of animals for this study was approved by the Animal Care and Use Committee at the Rocky Mountain Laboratories.