Activation of Apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx

Science

Volumn 281, Issue 5384, 1998, Pages 1860-1863

  Chang, Howard Y ^a   Nishitoh, Hideki ^b,c   Yang, Xiaolu ^a,e   Ichijo, Hidenori ^b,c   Baltimore, David ^a,d  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c CANCER INSTITUTE   (Japan)

^d CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^e UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTOR PROTEIN; CELL SURFACE RECEPTOR; PROTEIN KINASE; RECEPTOR PROTEIN; TRANSCRIPTION FACTOR;

AMINO TERMINAL SEQUENCE; APOPTOSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; KIDNEY CELL; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ALLELES; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD95; APOPTOSIS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CARRIER PROTEINS; CELL LINE; ENZYME ACTIVATION; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TUMOR CELLS, CULTURED;

EID: 0032544309     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5384.1860     Document Type: Article

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31. pEBB-Daxx (4), Daxx mutants (4), pEBB-Fas (4), pcDNA3-ASK1(8), pcDNA3-ASK1(K709R) (8), Myc-TAK1(6), pCMV-FLAG-JNK1(4), pRK5-crmA (20), EG202-ASK1(K709R) (12), and pJG4-5-mDaxx (4) were as described. ASK1ΔN, ΔC, kinase, FLAG-ASK1, Myc-ASK1, ASK1 (K709M)-HA, and Myc-ASKN were constructed in pcDNA3 (Invitrogen) by polymerase chain reaction (PCR). FLAG-tagged human Daxx and hDaxxC were derived from EST clone AA085057 and constructed in pRK5 (20) by PCR. pCI-AU1-hFas was constructed by J. Wang and M. J. Lenardo. The plasmids of GST-human MKK6, GST SAPK3/p38γ(KN), and GST-ASKN for bacterial fusion protein were constructed in pGEX-4T-1 (Pharmacia Biotech) by PCR.
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35. We are grateful to P. Svec for technical support. We thank S. Nagata, K. Matsumoto, J. Wang, M. J. Lenardo, D. V. Goeddel, M. Goeddert, and Z. Yao for reagents, and A. Hoffmann for valuable advice and critical review of the manuscript. H.I. thanks K. Miyazono for valuable discussion. H.Y.C. is supported by the Medical Scientist Training Program at Harvard Medical School. H.I. is supported by Grants-in-Aid for scientific research from the Ministry of Education, Science, and Culture of Japan. X.Y. is a fellow of the Leukemia Society of America. Supported by NIH grant CA51462.