Transformation abrogates an early G1-phase arrest point required for specification of the Chinese hamster DHFR replication origin

EMBO Journal

Volumn 17, Issue 6, 1998, Pages 1810-1818

  Wu, Jia Rui ^a,b   Keezer, Susan M ^a   Gilbert, David M ^a  

^a SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Cell cycle; DNA replication; Origin decision point; Protein kinase; Transformation

Indexed keywords

DIHYDROFOLATE REDUCTASE; PROTEIN KINASE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; CELL NUCLEUS; CHO CELL; CHROMATIN; CONTROLLED STUDY; DNA REPLICATION ORIGIN; DROSOPHILA; MITOSIS INHIBITION; NONHUMAN; PRIORITY JOURNAL; SIMIAN VIRUS 40; VIRUS CELL TRANSFORMATION; XENOPUS;

2-AMINOPURINE; ANIMALS; ANTIMETABOLITES; APHIDICOLIN; CELL TRANSFORMATION, VIRAL; CHO CELLS; CRICETINAE; DNA REPLICATION; ENZYME INHIBITORS; G1 PHASE; REPLICATION ORIGIN; S PHASE; SIMIAN VIRUS 40; TETRAHYDROFOLATE DEHYDROGENASE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0032536775     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.6.1810     Document Type: Article

References (32)

1. Anderson, S. and DePamphilis, M.L. (1979) Metabolism of Okazaki fragments during simian virus 40 DNA replication. J. Biol. Chem., 254, 11495-11564.
2. Burhans, W.C., Vassilev, L.T., Caddle, M.S., Heintz, N.H. and DePamphilis, M.L. (1990) Identification of an origin of bidirectional DNA replication in mammalian chromosomes. Cell, 62, 955-965.
3. Burhans, W.C., Vassilev, L.T., Wu, J., Sogo, J.M., Nallaseth, F.S. and DePamphilis, M.L. (1991) Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation. EMBO J., 10, 4351-4360.
4. Coverley, D. and Laskey, R.A. (1994) Regulation of eukaryotic DNA replication. Annu. Rev. Biochem., 63, 745-776.
5. 1-phase of the cell cycle. Proc. Natl Acad. Sci. USA, 88, 7580-7584.
6. DePamphilis, M.L. (1993) Origins of DNA replication in metazoan chromosomes. J. Biol. Chem., 268, 1-4.
7. Dijkwel, P.A. and Hamlin, J.L. (1995) The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites. Mol. Cell. Biol., 15, 3023-3031.
8. 1-phase of nontransformed mammalian cells are absent in transformed cells. Proc. Natl Acad. Sci. USA, 89, 8626-8630.
9. Gilbert, D.M. (1998) Replication origins in yeast vs. metazoa: separation of the haves and the have nots. Curr. Opin. Genet. Dev., 8, in press.
10. Gilbert, D. and Cohen, S. (1987) Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S-phase of the cell cycle. Cell, 50, 59-68.
11. Gilbert, D.M. and Cohen, S.N. (1990) Position effects on the timing of replication of chromosomally integrated simian virus 40 molecules in Chinese hamster cells. Mol. Cell. Biol., 10, 4345-4355.
12. Gilbert, D.M., Miyazawa, H. and DePamphilis, M.L. (1995) Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure. Mol. Cell. Biol., 15, 2942-2954.
13. Gruss, C., Wu, J., Koller, T. and Sogo, J.M. (1993) Disruption of the nucleosomes at the replication fork. EMBO J., 12, 4533-4545.
14. Hamlin, J.L. and Dijkwel, P.A. (1995) On the nature of replication origins in higher eukaryotes. Curr. Opin. Genet. Dev., 5, 153-161.
15. Hamlin, J.L., Mosca, P.J. and Levenson, V.V. (1994) Defining origins of replication in mammalian cells. Biochim. Biophys. Acta, 1198, 85-111.
16. Hyrien, O. and Mechali, M. (1993) Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos. EMBO J., 12, 4511-4520.
17. Hyrien, O., Maric, C. and Mechali, M. (1995) Transition in specification of embryonic metazoan DNA replication origins. Science, 270, 994-997.
18. Joo, W.S., Luo, X., Denis, D., Kim, H.Y., Rainey, G.J., Jones, C., Sreekumar, K.R. and Bullock, P.A. (1997) Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin. J. Virol., 71, 3972-3985.
19. Lawlis, S.J., Keezer, S.M., Wu, J.-R. and Gilbert, D.M. (1996) Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts. J. Cell Biol., 135, 1-12.
20. 1 nuclei in Xenopus egg extract. Cell, 69, 151-158.
21. Little, R. and Schildkraut, C. (1995) Initiation of latent DNA replication in the Epstein-Barr virus geneome can occur at sites other than the genetically defined origin. Mol. Cell. Biol., 15, 2893-2903.
22. Little, R.D., Platt, T.H. and Schildkraut, C.L. (1993) Initiation and termination of DNA replication in human rRNA genes. Mol. Cell. Biol., 13, 6600-6613.
23. Ludlow, J.W., DeCaprio, J.A., Huang, C.-M., Lee, W.-H., Paucha, E. and Livingston, D.M. (1989) SV40 large T antigen binds preferentially to an underphosphorylated member of the retinoblastoma susceptibility gene product family. Cell, 56, 57-65.
24. O'Keefe, R.T., Henderson, S.C. and Spector, D.L. (1992) Dynamic organization of DNA replication in mammalian cell nuclei - spatially and temporally defined replication of chromosome-specific α-satellite DNA sequences. J. Cell Biol., 116, 1095-1110.
25. Pelizon, C., Diviacco, S., Falaschi, A. and Giacca, M. (1996) High-resolution mapping of the origin of DNA replication in the hamster dihydrofolate reductase gene domain by competitive PCR. Mol. Cell. Biol. 16, 5358-5364.
26. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. 2nd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
27. Shinomiya, T. and Ina, S. (1993) DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites. Mol. Cell. Biol., 13, 4098-4106.
28. Shinomiya, T. and Ina, S. (1994) Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone. Mol. Cell. Biol., 14, 7394-7403.
29. 1 step required to specify the Chinese hamster DHFR replication origin. Science, 271, 1270-1272.
30. 1-phase step that precedes restriction point control. Mol. Cell. Biol., 17, 4312-4321.
31. Wu, J.-W., Yu, G. and Gilbert, D.M. (1997) Origin-specific initiation of mammalian nuclear DNA replication in a Xenopus cell-free system. METHODS, A Companion to Methods In Enzymology, 13, 313-324.
32. Zetterberg, A. and Larsson, O. (1995) What is the restriction point? Curr. Opin. Cell Biol., 7, 835-842.