Cloning and biochemical characterisation of Aspergillus niger hexokinase the enzyme is strongly inhibited by physiological concentrations of trehalose 6-phosphate

European Journal of Biochemistry

Volumn 258, Issue 1, 1998, Pages 223-232

  Panneman, Henk ^a   Ruijter, George J G ^a   Van Den Broeck, Hetty C ^a   Visser, Jaap ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Aspergillus niger; Expression; Glucokinase; Hexokinase; Purification; Trehalose 6 phosphate

Indexed keywords

ADENOSINE DIPHOSPHATE; ALPHA METHYLGLUCOSIDE; ARABINOSE; DEOXYGLUCOSE; FRUCTOSE; GALACTOSE; GLUCOKINASE; GLUCOSAMINE; GLUCOSE; HEXOKINASE; HEXOSE; MANNOSE; PENTOSE; SORBOSE; TREHALOSE; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CELL PH; DNA SEQUENCE; ENZYME CHEMISTRY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; HYBRIDIZATION; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ASPERGILLUS NIGER; EMERICELLA NIDULANS; EUKARYOTA; FUNGI; MYXOGASTRIA; SACCHAROMYCETALES;

EID: 0032533977     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2580223.x     Document Type: Article

References (49)

1. Albig, W. & Entian, K. D. (1988) Structure of yeast glucokinase, a strongly diverged specific aldohexose phosphorylating enzyme, Gene (Amst.) 73, 141-152.
2. Andreone, T. L., Printz, R. L., Pilkis, S. J., Magnuson, M. A. & Granner, D. K. (1989) The amino acid sequence of rat liver glucokinase deduced from cloned cDNA, J. Biol. Chem. 264, 363-369.
3. Arisan-Atac, I., Wolschek, M. F. & Kubicek, C. P. (1996) Trehalose-6-phosphate synthase A affects citrate accumulation by Aspergillus niger under conditions of high glycolytic flux, FEMS Microbiol. Lett. 140, 77-83.
4. Arora, K. K., Fanciulli, M. & Pedersen, P. L. (1990) Glucose phosphorylation in tumor cells: Cloning sequencing and overexpression in active form of a full-length cDNA encoding a mitochondrially bind-able form of hexokinase, J. Biol. Chem. 265, 6481-6488.
5. Barrel, B. G., Rajandream, M A. & Walsh, S. V. (1995) Swiss Ace. Q 09756.
6. Bensadoun, A. & Weinstein, D. (1976) Assay of proteins in the presence of interfering materials, Anal. Biochem. 70, 241-250.
7. Blázquez, M. A., Lagunas, R., Gancedo, C. & Gancedo, J. M. (1993) Trehalose-6-phosphate, a new regulator of yeast glycolysis that inhibits hexokinases, FEBS Lett. 329, 51-54.
8. Blázquez, M. A., Gancedo, J. M. & Gancedo, C. (1994) Use of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose-6-phosphate, FEMS Microbiol. Lett. 121, 223-228.
9. Cleland, W. W. (1963) The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations, Biochim. Biophys. Acta 67, 104-137.
10. Colowick, S. P. (1973) The hexokinases, in The enzymes (Boyer P. D., ed.) vol. 9B, pp. 1-48, Academic Press, New York and London.
11. De Graaff L., Van den Broek, H. & Visser, J. (1988) Isolation and expression of the Aspergillus nidulans pyruvate kinase gene, Curr. Genet. 13, 315-321.
12. Derechin, M., Ramel, A. R., Lazarus, N. R. & Barnard, E. A. (1966) Yeast hexokinase. II. Molecular mass and dissociation behaviour, Biochemistry 5, 4017-4026.
13. Easterby, J. S. & Rosemeyer, M. A. (1972) Purification and subunit interactions of yeast hexokinase, Eur. J. Biochem. 28, 241-252.
14. Felsenstein, J. (1993) k(Phylip) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle (USA).
15. Fröhlich, K. U., Entian, K. D. & Mecke, D. (1985) The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression, Gene (Amst.) 36, 105-111.
16. Goosen, T., Bloemheuvel, G., Gysler, C., De Bie, D. A., Van den Broek, H. W. J. & Swart, K. (1987) Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate-decarboxylase gene, Curr. Genet. 11, 499-503.
17. Griffin, L. D., MacGregor, G. R., Muzny, D. M., Harter, J., Cook, R. G. & McCabe, E. R. (1989) Synthesis and characterization of a bovine hexokinase I (HK1) cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures, Biochem. Med. Metab. Biol. 41, 125-131.
18. Hondmann, D. H. A. & Visser, J. (1990) Screening method for large numbers of dye-adsorbants for enzyme purification, J. Chromatogr. 510, 155-164.
19. Jang, J. C., Leon, P., Zhou, L. & Sheen, J. (1997) Hexokinase as a sugar sensor in higher plants, Plant Cell 9, 5-19
20. Kisser, M., Kubicek, C. P. & Röhr, M. (1980) Influence of manganese on morphology and cell wall composition of Aspergillus niger during citric acid fermentation, Arch. Microbiol. 128, 26-33.
21. Kopetzki, E. & Entian, K. D. (1985) Glucose repression and hexokinase enzymes in yeast. Isolation and characterization of a modified hexokinase PII isoenzyme, Eur. J. Biochem. 146, 657-662.
22. Koranyi, L. I., Tanizawa, Y., Welling, C. M., Rabin, D. U. & Permutt, M. A. (1992) Human islet glucokinase gene: Isolation and sequence analysis of full-length cDNA, Diabetes 41, 807-811.
23. Kusters-van Someren, M. A., Harmsen, J. A. M., Kester, H. C. M. & Visser, J. (1991) Structure of the Aspergillus niger pelA gene and its expression in Apergillus niger and Aspergillus nidulans, Curr. Genet. 20, 293-299.
24. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
25. Lobo, Z. & Maitra, P. K. (1977) Physiological role of glucose phosphorylating enzymes in Saccharomyces cerevisiae, Arch. Biochem. Biophys. 182, 639-645.
26. Magnuson, M. A. & Shelton, K. D. (1989) An alternate promoter in the glucokinase gene is active in the pancreatic beta cell, J. Biol. Chem. 264, 15936-15942.
27. Nishi, S., Seino, S. & Bell, G. I. (1988) Human hexokinase: Sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157, 937-943.
28. Olafsson, P., Matile, H. & Cesta, U. (1992) Molecular analysis of Plasmodium falciparum hexokinase, Mol. Biochem. Parasitol. 56, 89-102.
29. Panneman, H., Ruijter, G. J. G., Van den Broeck, H. C., Driever, E. T. M. & Visser, J. (1996) Cloning and biochemical characterisation of an Aspergillus niger glucokinase. Evidence for the presence of separate glucokinase and hexokinase enzymes, Eur. J. Biochem. 240, 518-525.
30. Petit, T., Blázquez, M. A. & Gancedo, C. (1996) Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases, FEBS Lett. 378, 185-189
31. Pontecorvo, G., Roper, J. A., Hemmons, L. J., MacDonald, K. J. & Bufton, A. W. J. (1953) The genetics of Aspergillus nidulans, Adv. Genet. 5, 141-238.
32. Prior, C., Mamessier, P., Fukuhara, H., Chen, X. J. & Wesolowski-Louvel, M. (1993) The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis, Mol. Cell. Biol. 13, 3882-3889.
33. Röhr, M., Stadler, P. J., Salzbrunn, W. O. J. & Kubicek, C. P. (1979) An improved method for characterization of citrate production by conidia of Aspergillus niger, Biotechnol. Lett. 1, 281-286.
34. Rose, M. (1995) Molecular and biochemical characterisation of the hexokinase from the starch-utilizing yeast Schwanniomvces occidentalis, Curr. Genet. 27, 330-338.
35. Ruijter, G. J. G., Panneman, H., Van den Broeck, H. C., Bennet, J. M. & Visser, J. (1996) Characterisation of the Aspergillus nidulans frA1 mutant: hexose phosphorylation and apparent lack of involvement of hexokinase in glucose repression, FEMS Microbiol. Lett 139, 223-228.
36. Ruijter, G. J. G. & Visser, J. (1996) Determination of intermediary metabolites in Aspergillus niger, J. Microbiol. Meth. 25, 295-302.
37. Ruijter, G. J. G., Panneman, H. & Visser, J. (1997) Overexpression of phosphofructokinase and pyruvate kinase in citric acid producing Aspergillus niger, Biochim. Biophys. Acta 1334, 317-326.
38. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edition, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
39. Schreferl-Kunar, G., Grotz, M., Röhr, M. & Kubicek, C. P. (1989) Increased citric acid production by mutants of Aspergillus niger with increased glycolytic capacity, FEMS Microbiol. Lett. 59, 297-300.
40. Schwab, D. A. & Wilson, J. E. (1989) Complete amino acid sequence of rat brain hexokinase. deduced from cloned cDNA, and proposed structure of a mammalian hexokinase, Proc. Natl Acad. Sci. USA 86, 2563-2567.
41. Schwab, D. A. & Wilson, J. E. (1991) Complete amino acid sequence of the type III isozyme of rat hexokinase, deducted from the cloned cDNA, Arch. Biochem. Biophys. 285, 365-370.
42. Shoemaker, C. B., Reynolds, S., Wei, G. & Harn, D. (1995) Schistosoma mansoni hexokinase: cDNA cloning and immunogenecity studies, Exp. Parasitai 80, 36-45.
43. Steinbock, F., Choojun, S., Held, I., Röhr, M. & Kubicek, C. P. (1994) Characterization and regulatory properties of a single hexokinase from the citric acid accumulating fungus Aspergillus niger, Biochim. Biophvs. Acta 1200, 215-223.
44. Tanizawa, Y., Koranyi, L. I., Welling, C. M. & Permutt, M. A. (1991) Human liver glucokinase gene: Cloning and sequence determination of two alternatively spliced cDNAs, Proc. Nutl Acad. Sci. USA 88, 7294-7297.
45. Thelen, A. P. & Wilson, J. E. (1991) Complete amino acid sequence of the type II isoenzyme of rat hexokinase, deduced from the cloned cDNA: Comparison with a hexokinase from Novikoff ascites tumor, Arch. Biochem. Biophys. 286, 645-651.
46. Thevelein, J. M. & Hohmann, S. (1995) Trehalose synthase: Guard to the gate of glycolysis in yeast? Trends Biochem. Sci. 20, 3-10.
47. Torres, N. V. (1994a) Modeling approach to control of carbohydrate metabolism during citric acid accumulation by Aspergillus niger: I Model definition and stability of the steady state, Biotechnol. Bioeng. 44, 104-111.
48. Torres, N. V. (1994b) Modeling approach to control of carbohydrate metabolism during citric acid accumulation by Aspergillus niger: II Sensitivity analysis, Biotechnol. Bioeng. 44, 112-118.
49. Unkles, S. E. (1992) Gene organisation in filamentous fungi, in Applied molecular genetics of filamentous fungi (Kinghorn. J. R. & Turner, G., eds) pp. 28-53, Blackie Academic and Professional, Glasgow.