Reconstitution of recombinant bovine A1 adenosine receptors in Sf9 cell membranes with recombinant G proteins of defined composition

Molecular Pharmacology

Volumn 50, Issue 6, 1996, Pages 1587-1595

  Figler, Robert A ^a   Graber, Stephen G ^b   Lindorfer, Margaret A ^a   Yasuda, Hiroshi ^a   Linden, Joel ^a   Garrison, James C ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

^b WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 (4 AMINO 3 IODOBENZYL) 8 CYCLOPENTYL 1 PROPYLXANTHINE; 6 N (4 AMINOBENZYL)ADENOSINE; ADENOSINE 5' (N ETHYLCARBOXAMIDE); ADENOSINE A1 RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; PHENYLISOPROPYLADENOSINE; PROTEIN SUBUNIT; RADIOLIGAND; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACULOVIRUS; BINDING AFFINITY; BINDING COMPETITION; BRAIN MEMBRANE; CATTLE; CELL LINE; CELL MEMBRANE; CONTROLLED STUDY; DRUG RECEPTOR BINDING; GENE EXPRESSION; INSECT; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS RECOMBINANT;

ANIMALS; CATTLE; CELL LINE; CLONING, MOLECULAR; GTP-BINDING PROTEINS; RADIOLIGAND ASSAY; RECEPTORS, PURINERGIC P1; RECOMBINANT PROTEINS; SPODOPTERA;

EID: 0030463177     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

1. 1-adenosine receptor. Mol. Pharmacol. 35: 450-457 (1989).
2. 1 adenosine receptors and guanine nucleotide-binding proteins from bovine brain. J. Biol. Chem. 264: 14853-14859 (1989).
3. 1-adenosine receptors with G proteins of human platelet membranes following reconstitution. Mol. Pharmacol. 38: 170-176 (1990).
4. 1-adenosine receptor with recombinant G protein α-subunits. Selectivity for rGi α-3. J. Biol. Chem. 266: 17778-17783 (1991).
5. 1 adenosine receptors of bovine brain couple to guanine nucleotide-binding proteins Gil, Gi2, and Go. J. Biol. Chem. 266: 22285-22289 (1991).
6. Birnbaumer, L. Receptor-to-effector signaling through G proteins: roles for beta gamma dimers as well as alpha subunits. Cell 71: 1069-1072 (1992).
7. Watson, A. J., A. Katz, and M. I. Simon. A fifth member of the mammalian G-protein β-subunit family. J. Biol. Chem. 269: 22150-22156 (1994).
8. Clapham, D. E. and E. J. Neer. New roles for G-protein beta gammadimers in transmembrane signalling. Nature (Lond.) 365:403-406 (1993).
9. Ray, K., C. Kunsch, L. M. Bonner, and J. D. Robishaw. Isolation of cDNA clones encoding eight different human G protein y subunits, including three novel forms designated the γ 4, γ 10, and γ 11 subunits. J. Biol. Chem. 270: 21765-21771 (1995).
10. Morishita, R., H. Nakayama, T. Isobe, T. Matsuda, Y. Hashimoto, T. Okano, Y. Fukada, K. Mizuno, S. Ohno, O. Kozawa, K. Kato, and T. Asano. Primary structure of a γ subunit of G protein, γ12, and its phosphorylation by protein kinase C. J. Biol. Chem. 270: 29469-29475 (1995).
11. αo increases its affinity for βγ. J. Biol. Chem. 266: 4654-4659 (1991).
12. 2-adrenergic receptor. Mutation of Cys-341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor. J. Biol. Chem. 264: 7564-7569 (1989).
13. Mumby, S. M., C. Kleuss, and A. G. Gilman. Receptor regulation of G-protein palmitoylation. Proc. Natl. Acad. Sci. USA 91: 2800-2804 (1994).
14. Muntz, K. H., P. C. Sternweis, A. G. Gilman, and S. M. Mumby. Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes. Mol. Biol. Cell 3: 49-61 (1992).
15. Wedegaertner, P. B., P. T. Wilson, and H. R. Bourne, Lipid modifications of trimeric G proteins. J. Biol. Chem. 270: 503-506 (1995).
16. Graber, S. G., R. A. Figler, and J. C. Garrison. Expression and purification of functional G protein α subunits using a baculovirus expression system. J. Biol. Chem. 267: 1271-1278 (1992).
17. Graber, S. G., R. A. Figler, V. K. Kalman-Maltese, J. D. Robishaw, and J. C. Garrison. Expression of functional G protein βγ dimers of defined subunit composition using a baculovirus expression system. J. Biol. Chem. 267: 13123-13126 (1992).
18. Parker, E. M., K. Kameyama, T. Higashijima, and E. M. Ross. Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J. Biol. Chem. 266: 519-527 (1991).
19. Kalman, V. K., R. A. Erdman, W. A. Maltese, and J. D. Robishaw. Regions outside of the CAAX motif influence the specificity of prenylation of G protein γ subunits. J. Biol. Chem. 270: 14835-14841 (1995).
20. 1 receptor cDNA. FEBS Lett. 297: 107-111 (1992).
21. Summers, M. D. and G. E. Smith. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin 1555, Texas A&M University, College Station, Texas (1988).
22. 125I]-azido-BW-A844U, a photoaffinity label. Mol. Pharmacol. 33: 585-591 (1988).
23. Boyer, J. L., S. G. Graber, G. L. Waldo, T. K Harden, and J. C. Garrison. Selective activation of phospholipase C by recombinant G-protein α- and βγ-subunits. J. Biol. Chem. 269: 2814-2819 (1994).
24. Tucker, A. L., A. S. Robeva, H. E. Taylor, D. Holeton, M. Bockner, K. R. Lynch, and J. Linden. A1 adenosine receptors. Two amino acids are responsible for species differences in ligand recognition. J. Biol. Chem. 269: 27900-27906 (1994).
25. Fung, B. K. Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits. J. Biol. Chem. 258: 10495-10502 (1983).
26. 1-adrenoceptor to adenylate cyclase by γ subunits in a reconstituted system. Eur. J. Biochem. 169: 431-439 (1987).
27. Florio, V. A. and P. C. Sternweis. Mechanisms of muscarinic receptor action on Go in reconstituted phospholipid vesicles. J. Biol. Chem. 264: 3909-3915 (1989).
28. Mulheron, J. G., S. J. Casanas, J. M. Arthur, M. N. Garnovskaya, T. W. Gettys, and J. R. Raymond. Human 5-HT1A receptor expressed in insect cells activates endogenous G(o)-like G protein(s). J. Biol. Chem. 269: 12954-12962 (1994).
29. Ueda, N., J. A. Iniguez-Lluhi, E. Lee, A. V. Smrcka, J. D. Robishaw, and A. G. Gilman. G protein βγ subunits. Simplified purification and properties of novel isoforms. J. Biol. Chem. 269: 4388-4395 (1994).
30. 2-adrenergic receptor in Sf9 cells. J. Biol. Chem. 267: 21733-21737 (1992).
31. Ng, G. Y., B. Mouillac, S. R. George, M. Caron, M. Dennis, M. Bouvier, and B. F. O'Dowd. Desensitization, phosphorylation and palmitoylation of the human dopamine D1 receptor. Eur. J. Pharmacol. 267: 7-19 (1994).
32. Parker, E. M., D. A. Grisel, L. G. Iben, H. P. Nowak, C. D. Mahle, F. D. Yocca, and G. T. Gaughan. Characterization of human 5-HT1 receptors expressed in Sf9 insect cells. Eur. J. Pharmacol. 268: 43-53 (1994).
33. 1-adenosine receptor. J. Biol. Chem. 269: 32077-32084 (1994).
34. Wilcox, M. D., K. L. Schey, J. Dingus, N. D. Mehta, B. S. Tatum, M. Halushka, J. W. Finch, and J. D. Hildebrandt. Analysis of G protein γ subunit heterogeneity using mass spectrometry. J. Biol. Chem. 269: 12508-12513 (1994).
35. Asano, T., R. Morishita, T. Matsuda, Y. Fukada, T. Yoshizawa, and K. Kato. Purification of four forms of the βγ subunit complex of G proteins containing different γ subunits. J. Biol. Chem. 268: 20512-20519 (1993).
36. Lambright, D. G., J. Sondek, A. Bohm, N. P. Skiba, H. E. Hamm, and P. B. Sigler. The 2.0 angstrom crystal structure of a heterotrimeric G protein. Nature (Lond.) 379: 311-319 (1996).
37. 1a receptor in Sf9 cells, reconstitution of a coupled phenotype by co-expression of mammalian G protein subunits. J. Biol. Chem. 270:18691-9: (1995).
38. Yamane, H. K., C. C. Farnsworth, H. Y. Xie, W. Howald, B. K. Fung, S. Clarke, M. H. Gelb, and J. A. Glomset. Brain G protein γ subunits contain an all-trans-geranylgeranylcysteine methyl ester at their carboxyl termini. Proc. Natl. Acad. Sci. USA 87: 5868-5872 (1990).
39. Mumby, S. M., P. J. Casey, A. G. Gilman, S. Gutowski, and P. C. Sternweis. G protein γ subunits contain a 20-carbon isoprenoid. Proc. Natl. Acad. Sci. USA 87: 5873-5877 (1990).
40. Fukada, Y., T. Takao, H. Ohguro, T. Yoshizawa, T. Akino, and Y. Shimonishi. Farnesylated γ-subunit of photoreceptor G protein indispensable for GTP-binding. Nature (Lond.) 346: 658-660 (1990).
41. 1 adenosine receptors. J. Biol. Chem. 271: 18588-18595 (1996).
42. Kisselev, O. and N. Gautam. Specific interaction with rhodopsin is dependent on the γ subunit type in a G protein. J. Biol. Chem. 268: 24519-24522 (1993).
43. Kleuas, C., J. Hescheler, C. Ewel, W. Rosenthal, G. Schultz, and B. Wittig. Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. Nature (Lond.) 363: 43-48 (1991).
44. Kleuss, C., H. Scherubl, J. Hescheler, G. Schultz, and B. Wittig. Different β-subunits determine G-protein interaction with transmembrane receptors. Nature (Lond.) 358: 424-426 (1992).
45. Kleuss, C., H. Scherubl, J. Hescheler, G. Schultz, and B. Wittig. Selectivity in signal transduction determined by γ subunits of heterotrimeric G proteina. Science (Washington D. C.) 259: 832-834 (1993).
46. Pronin, A. N. and N. Gautam. Interaction between G-protein β and γ subunit types is selective. Proc. Natl. Acad. Sci. USA 89: 6220-6224 (1992).
47. Schmidt, C. J., T. C. Thomas, M. A. Levine, and E. J. Neer. Specificity of G protein β and γ subunit interactions. J. Biol. Chem. 267: 13807-13810 (1992).
48. Spring, D. J. and E. J. Neer. A 14-amino acid region of the G-protein γ subunit if sufficient to confer selectivity of γ binding to the β subunit. J. Biol. Chem. 269: 22882-22886 (1994).
49. Gudermann, T., F. Kalkbrenner, and G. Schultz. Diversity and selectivity of receptor-G protein interaction. Annu. Rev. Pharmacol. Toxicol. 36: 429-459 (1996).
50. Neubig, R. R. Membrane organization in G-protein mechanisms. FASEB J. 8: 939-946 (1994).
51. Marquardt, D. M. An algorithm for least-squares estimation of nonlinear parameters. J. Soc. Ind. Appl. Math. 11: 431-441 (1963).
52. Motulsky, H. J. and L. A. Ransnas. Fitting curves to data using nonlinear regression: a practical and nonmathematical review. FASEB J. 1: 365-374 (1987).
53. Linden, J. Calculating the dissociation constant of an unlabeled compound from the concentration required to displace radiolabel binding by 50%. J. Cyclic Nucleotide Res. 8: 163-172 (1982).