GTP-binding protein γ12 subunit phosphorylation by protein kinase C - Identification of the phosphorylation site and factors involved in cultured cells and rat tissues in vivo

European Journal of Biochemistry

Volumn 251, Issue 1-2, 1998, Pages 314-319

  Asano, Tomiko ^a   Morishita, Rika ^a   Ueda, Hiroshi ^a   Asano, Masahisa ^b   Kato, Kanefusa ^a  

^a INSTITUTE FOR DEVELOPMENTAL RESEARCH   (Japan)

^b NAGOYA CITY UNIV MEDICAL SCHOOL   (Japan)

Author keywords

GTP binding protein; Lysophosphatidic acid; Protein kinase C; Rat tissue; Swiss 3T3 fibroblast

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; LYSOPHOSPHATIDIC ACID; PHORBOL 13 ACETATE 12 MYRISTATE; PLATELET DERIVED GROWTH FACTOR; PROTEIN KINASE C;

ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL STRAIN 3T3; CONTROLLED STUDY; ENZYME SUBSTRATE COMPLEX; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

3T3 CELLS; ANIMALS; ANTIBODIES; BINDING SITES; GTP-BINDING PROTEINS; LYSOPHOSPHOLIPIDS; MICE; OXAZOLES; PEPTIDE FRAGMENTS; PERTUSSIS TOXIN; PHOSPHORYLATION; PLATELET-DERIVED GROWTH FACTOR; PROTEIN KINASE C; RATS; RATS, WISTAR; SERINE; TETRADECANOYLPHORBOL ACETATE; VIRULENCE FACTORS, BORDETELLA;

EID: 0032518292     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2510314.x     Document Type: Article

References (34)

1. Neer, E. J. (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80, 249-257.
2. Iñiguez-Lluhi, J., Kleuss, C. & Gilman, A. G. (1993) The importance of G-protein βγ subunits, Trends Cell Biol. 3, 230-236.
3. Crespo, P., Xu, N., Simonds, W. F. & Gutkind, J. S. (1994) Ras-dependent activation of MAP kinase pathway mediated by G-protein βγ subunits, Nature 369, 418-420.
4. Faure, M., Voyno-Yasenetskaya, T. A. & Bourne, H. R. (1994) cAMP and βγ subunits of heterotrimeric G proteins stimulate the mitogen-activated protein kinase pathway in COS-7 cells, J. Biol. Chem. 269, 7851-7854.
5. Hurley, J. B., Fong, H. K. W., Teplow, D. B., Dreyer, W. J. & Simon, M. I. (1984) Isolation and characterization of a cDNA clone for the gamma subunit of bovine retinal transducin. Proc. Natl Acad. Sci. USA 81, 6948-6952.
6. Yatsunami, K., Pandya, B. V., Oprian, D. D. & Khorana, H. G. (1985) cDNA-derived amino acid sequence of the γ subunit of GTPase from bovine rod outer segments. Proc Natl Acad. Sci. USA 82, 1936-1940.
7. Gautam, N., Baetscher, M., Aebersold, R. & Simon, M. I. (1989) A G protein γ subunit shares homology with ras proteins, Science 244, 971-974.
8. Robishaw, J. D., Kalman, V. K., Moomaw, C. R. & Slaughter, C. A. (1989) Existence of two γ subunits of the G proteins in brain, J. Biol. Chem. 264, 15758-15761.
9. Gautam, N., Northup, J., Tamir, H. & Simon, M. I. (1990) G protein diversity is increased by associations with a variety of γ subunits, Poc. Natl Acad. Sci. USA 87, 7973-7977.
10. 5), Mol. Cell. Biol. 12, 1585-1591.
11. Cali, J. J., Balcueva, E. A., Rybalkin, I. & Robishaw, J. D. (1992) Selective tissue distribution of G protein γ subunits, including a new form of the γ subunits identified by cDNA cloning, J. Biol. Chem. 267, 24023-24027.
12. Ryba, N. J. P. & Tirindelli, R. (1995) A novel GTP-binding protein γ-subunit Gγ8, is expressed during neurogenesis in the olfactory and vomeronasal neuroepithelia, J. Biol. Chem. 270, 6757-6767.
13. Ong, O. C., Yamane, H. K., Phan, K. B., Fong, H. K. W., Bok, D., Lee, R. H. & Fung, B. K.-K. (1995) Molecular cloning and characterization of the G protein γ subunit of cone photoreceptors, J. Biol. Chem. 270, 8495-8500.
14. 11 subunits, J. Biol. Chem. 270, 21765-21771.
15. 12, and its phosphorylation by protein kinase C. J. Biol. Chem. 270, 29469-29475.
16. Iñiguez-Lluhi, J. A., Simon, M. I., Robishaw, J. D. & Gilman, A. G. (1992) G protein βγ subunits synthesized in Sf9 cells: functional characterization and the significance of prenylation of γ, J. Biol. Chem. 267, 23409-23417.
17. Asano, T., Morishita, R., Matsuda, T., Fukada, Y. Yoshizawa, T. & Kato, K. (1993) Purification of four forms of the βγ subunit complex of G proteins containing different γ subunits, J. Biol. Chem. 268, 20512-20519.
18. Kisselev, O. & Gautam, N. (1993) Specific interaction with rhodopsin is dependent on the γ subunit type in a G protein, J. Biol. Chem. 268, 24519-24522.
19. Ueda, N., Iñiguez-Lluhi, J. A., Lee, E., Smrcka, A. V., Robishaw, J. D. & Gilman, A. G. (1994) G protein βγ subunits: simplified purification and properties of novel isoforms, J. Biol. Chem. 269, 4388-4395.
20. 12 subunits of G proteins in the mammalian brain. J. Neurochem. 68, 820-827.
21. Asano, T., Morishita, R., Ohashi, K., Nagahama, M., Miyake, T. & Kato, K. (1995) Localization of various forms of the γ subunit of G protein in neural and nonneural tissues, J. Neurochem. 64, 1267-1273.
22. Berridge, M. J. & Irvine, R. F. (1989) Inositol phosphates and cell signalling, Nature 341, 197-205.
23. Nishizuka, Y. (1986) Studies and perspectives of protein kinase C, Science 233, 305-312.
24. Marglin, A. & Merrifield, R. B. (1970) Chemical synthesis of peptides and proteins, Annu. Rev. Biochem. 39, 841-866.
25. Schägger, H. & von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem. 166, 368-379.
26. 1 receptor and pertussis toxin-sensitive G protein on the ferret ventricular contraction, Naunyn-Schmiedeberg's Arch. Pharmacol. 348, 282-289.
27. Ridley, A. J. & Hall, A. (1992) The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors, Cell 70, 389-399.
28. Ha, K.-S. & Exton, J. H. (1993) Differential translocation of protein kinase C isozymes by thrombin and platelet-derived growth factor: a possible function for phosphatidylcholine-derived diacylglycerol, J. Biol. Chem. 268, 10534-10539.
29. Graff, J. M., Stumpo, D. J. & Blackshear, P. J. (1989) Characterization of the phosphorylation sites in the chicken and bovine myristoylated alanine-rich C kinase substrate protein, a prominent cellular substrate for protein kinase C, J. Biol. Chem. 264, 11912-11919.
30. Exton, J. H. (1997) Cell signalling through guanine-nucleolide-binding regulatory proteins (G proteins) and phospholipases, Eur. J. Biochem. 243, 10-20.
31. Moolenaar, W. H. (1995) Lysophosphatidic acid, a multifunctional phospholipid messenger, J. Biol. Chem. 270, 12949-12952.
32. 2+ mobilization in human A431 cells: structure-activity analysis, Biochem. J. 307, 609-616.
33. van Corven, E. J., Groenink, A., Jalink, K., Eichholtz, T. & Moolenaar, W. H. (1989) Lysophosphatidate-induced cell proliferation: identification and dissection of signaling pathways mediated by G proteins, Cell 59, 45-54.
34. Ui, M. (1984) Islet-activating protein, pertussis toxin: a probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase, Trends Pharmacol. Sci. 5, 277-279.